Fibronectin adsorption or/and covalent grafting on chemically modified PEEK film surfaces

Abstract

Poly(ether ether ketone) (PEEK) films were chemically modified, by surface wet chemistry, into PEEK-OH, PEEK-NH2, and PEEK-NCO. Fibronectin (FN) adsorption, in the presence or absence of two non-ionic surfactants, was compared onto PEEK, PEEK-OH, and PEEK-NH2 on which the protein can only be adsorbed, and onto PEEK-NCO on which FN could be covalently grafted. The amounts of FN present on the various supports were assayed by ELISA and LSC (with 125I-labeled FN). The remarkable effect of Pluronic F68 in preventing non-specific protein adhesion on the less hydrophilic surfaces was pointed out. Accordingly, a procedure could be proposed that allows minimal FN adhesion vs FN fixation on PEEK-NCO. The resulting PEEK-FN film, which immobilized 120-150 ng FN cm(-2), constitutes a new substratum for cell cultivation.

Effect of Local Sustainable Release of BMP2-VEGF from Nano-Cellulose Loaded in Sponge Biphasic Calcium Phosphate on Bone Regeneration

“…Cellulose fiber has a high density of reactive hydroxyl groups on its surface, which facilitates the immobilization of cell adhesive proteins such as fibronectin. 54 Addition of BMP2 or VEGF enhanced the proliferation, which was highest with the combination of BMP2 and VEGF. BMP2 affects the interactions between the scaffolds and cell surface receptors and activates cell proliferation via the MARK Erk pathway.…”

PEEK biomaterials in trauma, orthopedic, and spinal implants

“…Plasma deposition processing techniques are compatible with PEEK [96,108]. Surface modification of PEEK may also be employed by wet chemistry [109,110] and by plasma treatment [111,112] to improve biocompatibility.…”

Current Strategies to Improve the Bioactivity of PEEK

“…They are hydroxylated polymer (PEEK–OH) obtained by reduction, carboxylated polymer (PEEK–NCO) prepared by coupling a diisocyanate reagent to PEEK–OH, aminated polymer (PEEK–NH 2 ) acquired by hydrolysis of PEEK–NCO, and aminocarboxylated polymers (PEEK–GABA and PEEK–Lysine) resulting from the coupling of aminoacids to PEEK–NCO [36,37]. These chemical modifications promoted higher levels of fibronectin covalently fixed and/or adsorbed on various treated PEEK compared with untreated PEEK.…”