Fibronectin: A review of its structure and biological activity

Pearlstein, Edward; Gold, Leslie I.; García-Pardo, Ángeles

doi:10.1007/bf00220304

Cited by 353 publications

(140 citation statements)

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“…Its importance in the mediation of cell adhesion was early recognized 12. It is a dimer consisting of two subunits of ≈220 kDa, each containing three types of repeating units that mediate interactions with ECM proteins, several members of the integrin family, and other molecules.…”

Section: Introductionmentioning

confidence: 99%

Vitronectin as a Micromanager of Cell Response in Material‐Driven Fibronectin Nanonetworks

et al. 2017

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Surface functionalization strategies of synthetic materials for regenerative medicine applications comprise the development of microenvironments that recapitulate the physical and biochemical cues of physiological extracellular matrices. In this context, material‐driven fibronectin (FN) nanonetworks obtained from the adsorption of the protein on poly(ethyl acrylate) provide a robust system to control cell behavior, particularly to enhance differentiation. This study aims at augmenting the complexity of these fibrillar matrices by introducing vitronectin, a lower‐molecular‐weight multifunctional glycoprotein and main adhesive component of serum. A cooperative effect during co‐adsorption of the proteins is observed, as the addition of vitronectin leads to increased fibronectin adsorption, improved fibril formation, and enhanced vitronectin exposure. The mobility of the protein at the material interface increases, and this, in turn, facilitates the reorganization of the adsorbed FN by cells. Furthermore, the interplay between interface mobility and engagement of vitronectin receptors controls the level of cell fusion and the degree of cell differentiation. Ultimately, this work reveals that substrate‐induced protein interfaces resulting from the cooperative adsorption of fibronectin and vitronectin fine‐tune cell behavior, as vitronectin micromanages the local properties of the microenvironment and consequently short‐term cell response to the protein interface and higher order cellular functions such as differentiation.

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Section: Introductionmentioning

confidence: 99%

Vitronectin as a Micromanager of Cell Response in Material‐Driven Fibronectin Nanonetworks

et al. 2017

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“…Pearlstein et al 3 showed that the cells of the epithelial crypts in the small bowel of rats synthesized FN in vitro and found this protein in the basal membrane of the intestine, suggesting its synthesis in vivo.…”

Section: Discussionmentioning

confidence: 99%

Effect of topical application of fibronectin in duodenal wound healing in rats

Brito¹,

Rocha²,

Jatobá³

et al. 2003

Acta Cir. Bras.

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Brito TNS, Rocha LRM, Jatobá CAN, Sales MP, Medeiros AC. Effect of topical application of fibronectin in duodenal wound healing in rats. Acta Cir Bras [serial online] 2003 Mar-Apr;18(2). Available from URL: http://www.scielo.br/acb. ABSTRACT -Fibronectin (FN), a large family of plasma and extracellular matrix glycoproteins, plays an important role in wound healing. Purpose: To evaluate the effect of fibronectin on the healing of sutured duodenal wounds, correlating with the serum and tissue level of the substance. Methods: An experimental study was done in 30 adult Wistar rats divided into two group. In the control group (n=15) a duodenal suture was treated with saline solution 0,9% and in the test group the duodenal wounds were treated with 1% FN. The duodenal wound healing process was studied in the 5 th , 7 tn and 10 th postoperative days, by histological sections stained by hematoxylin-eosin, Masson trichromic and immunohistochemical reaction for FN. A digital histological grading system was used to obtain a score for each group and to observe the healing process. Results: the FN was present in the several layers of the duodenum and the cellular and plasmatic FN increased with the evolution of healing. In the test group the FN enhanced the wound healing within 5, 7 and 10 days after injury, when compared with the control group. Conclusion: The topical use of FN in duodenal sutured wounds in rats enhances healing by stimulating the appearence of fibroblasts into the wound site and development of granulation tissue. This acceleration of the repair process may have an important application in the healing of duodenal wounds.

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“…Fibronectin is a high-M r glycoprotein which is found in the blood plasma and other body fluids, in connective tissues and in basement membrane [1][2][3][4][5][6][7]. Fibronectin is involved in a variety of cell functionsadhesion on different surfaces, cell motility, phagocytosis [1][2][3][4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

“…Fibronectin is involved in a variety of cell functionsadhesion on different surfaces, cell motility, phagocytosis [1][2][3][4][5][6][7]. Fibronectin is a multidomain disulfidebonded dimer, the dimensions of the molecule are 15 X 8 nm;/3-form is the main element of secondary structure [8,9].…”

Section: Introductionmentioning

confidence: 99%

“…Fibronectin is a multidomain disulfidebonded dimer, the dimensions of the molecule are 15 X 8 nm;/3-form is the main element of secondary structure [8,9]. Fibronectin has an affinity to the cell surface, collagen, heparin, fibrinogen, DNA, actin and some other macromolecules [1][2][3][4][5][6][7]. Similar chemical properties and functions are characteristic for fibronectins isolated from different sources.…”

Section: Introductionmentioning

confidence: 99%

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