Fibronectin 1 inhibits the apoptosis of human trophoblasts by activating the PI3K/Akt signaling pathway

Abstract

The excessive apoptosis of human trophoblasts can cause pregnancy-related diseases. It has been reported that fibronectin 1 (FN1) is closely associated with the invasion of human trophoblasts. The aim of the present study was to examine the effects of FN1 on the apoptosis of human trophoblasts and to investigate the underlying molecular mechanisms. It was found that FN1, a differentially expressed gene (DEG) in the GSE127170 dataset, was identified as the hub gene in a protein-protein interaction (PPI) network generated using the cytoHubba plug-in of Cytoscape software. The Metascape website was used to perform GO enrichment analysis, and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database was used to perform KEGG pathway analysis. Experimental analyses revealed that FN1 expression was downregulated in the chorionic villus tissues of patients diagnosed with and mice subjected to spontaneous abortion (SA). CCK-8 and flow cytometric assays revealed that the knockdown of FN1 decreased the viability and promoted the apoptosis of JEG-3 and BeWo cells. In vivo experiments demonstrated that the knockdown of FN1 promoted the apoptosis of trophoblasts in the chorionic villus tissues obtained from mice subjected to SA, whereas FN1 overexpression increased cell viability and inhibited cell apoptosis. The protein levels of cleaved caspase-3 and Bax were increased by the silencing of FN1 and decreased by FN1 overexpression. The protein expression levels of Bcl-2, proliferating cell nuclear antigen (PCNA) and Ki67 were decreased by the silencing of FN1; however, the overexpression of FN1 increased these levels. The results of western blot analysis revealed that the knockdown of FN1 inhibited the PI3K/Akt signaling pathway, while the overexpression of FN1 activated the PI3K/Akt signaling pathway. Consistently, the apoptosis-inhibiting effect of FN1 overexpression was reversed by a PI3K/Akt signaling pathway inhibitor, and the apoptosis-promoting effect of FN1 silencing was reversed by a PI3K/Akt signaling pathway activator. On the whole, the findings of the present study demonstrate that the inhibition of FN1 induces the apoptosis of JEG-3 and BeWo cells, and the overexpression of FN1 inhibits cell apoptosis by activating the PI3K/Akt signaling pathway.

A genome-wide CRISPR-Cas9 knockout screen identifies essential and growth-restricting genes in human trophoblast stem cells

“…2n ). A number of key trophoblast regulators displayed significant chromatin accessibility changes consistent with reduced or increased expression in TEAD1 KO hTSCs, including ZFP57 58 , FN1 51 (downregulated), and CBR1 59 (upregulated) (Fig. 2o ).…”

“…There were 162 genes significantly downregulated in TEAD1 KO hTSCs ( p -adjust < 0.05) (Fig. 2l ), including many trophoblast regulators such as FOS 49 , 50 , FN1 51 , ITGB8 52 , MET 53 , and LOXL2 54 . Genes downregulated in TEAD1 KO hTSCs were enriched for GO terms such as integrin-mediated signaling pathway, regulation of TGF-β production, and regulation of cell motility (Fig.…”

LINC02381 aggravates breast cancer through the miR‐1271‐5p/FN1 axis to activate PI3K/AKT pathway

“…FN1 was found to regulate activation of PI3K/AKT pathway in many diseases 23–25 . Thus, we intended to verify whether FN1 could regulate this signaling in BC.…”

“…Additionally, the inhibitory action of LINC02381 depletion on cell process in BC was abrogated by FN1 overexpression. FN1 was a positive regulator of PI3K/AKT pathway in many diseases 23–25 . PI3K/Akt is a key intracellular pathway modulating many biological processes, including cell survival, apoptosis, and metastasis 38 .…”

GATA6‑induced FN1 activation promotes the proliferation, invasion and migration of oral squamous cell carcinoma cells

“…Quantitative proteomics analyses revealed FN1 as a candidate biomarker of sporadic medullary thyroid cancer ( 36 ) and FN1 is also considered as a potential biomarker in OSCC regarding the tongue, mouth floor and edentulous ridge ( 15 ). Ji et al ( 37 ) revealed that the protein expression levels of Ki67 and PCNA is decreased by the silencing of FN1 in human trophoblasts. FN1 knockdown represses cell proliferation, invasion and migration of nasopharyngeal carcinoma cells, coupled with downregulated expression of MMP2 and MMP9 ( 26 ).…”