Fibronectin 1 activates WNT/β-catenin signaling to induce osteogenic differentiation via integrin β1 interaction

Yang, Chengliang; Wang, Chong; Zhou, Jing; Liang, Qingyang; He, Feng; Li, Feng; Li, Ye; Chen, Jinzhong; Zhang, Fan; Han, Chao; Liu, Jia; Li, Kai; Tang, Yue

doi:10.1038/s41374-020-0451-2

Cited by 38 publications

(28 citation statements)

References 37 publications

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“…OSX is expressed in all types of developing bone cells and inhibits differentiation of progenitor cells to chondrocytes [41]. FN is a large glycoprotein that binds to cells and engages in cellular processes including wound healing, cell migration, and cytoskeleton organization [42], which also contributes to osteoblast survival and differentiation. OC, as a type of bone non-collagenous protein, is synthesized at the late stage of osteoblast differentiation [43].…”

Section: Discussionmentioning

confidence: 99%

Probiotic Propionibacterium freudenreichii MJ2 Enhances Osteoblast Differentiation and Mineralization by Increasing the OPG/RANKL Ratio

Yeom

Lim

2021

Microorganisms

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Osteoblast differentiation is important for the development of bone and the maintenance of bone density. Propionibacterium freudenreichii is a probiotic with an anti-inflammatory property. The aim of this study was to investigate the enhancement effect of P. freudenreichii MJ2 (MJ2) isolated from raw milk on osteoblast differentiation, mineralization, and its signaling pathway. For in vitro and in vivo experiments, human fetal osteoblastic cell line hFOB 1.19 and an ovariectomized rat model were used, respectively. Expression levels of genes and proteins related to osteoblast differentiation and mineralization were measured by real-time polymerase chain reaction (qPCR) and Western blotting, respectively. Alizarin red S staining was performed to measure osteoblast mineralization. Heat-killed MJ2 (hkMJ2)-treated cells showed significantly increased osteoblast differentiation via an increase in the osteoprotegerin (OPG)/receptor activator of nuclear factor-κB ligand (RANKL) ratio and significantly increased osteoblast mineralization by stimulating the expression of bone morphogenetic protein 2 and runt-related transcription factor 2. Additionally, oral administration of live or heat-killed MJ2 to ovariectomized rats inhibited osteoporosis-induced bone loss. Specifically, surface proteins isolated from MJ2 promoted osteoblast differentiation and mineralization. In conclusion, MJ2 enhanced osteoblast differentiation and mineralization through the OPG/RANKL signaling pathway and the effective component of MJ2 might be its surface proteins.

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Section: Discussionmentioning

confidence: 99%

Probiotic Propionibacterium freudenreichii MJ2 Enhances Osteoblast Differentiation and Mineralization by Increasing the OPG/RANKL Ratio

Yeom

Lim

2021

Microorganisms

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“…To detect how the D/PCL-2 scaffold influences the process of endothelial remodeling, HUVECs were seeded on a D/PCL-2 scaffold to detect relative protein expression levels using RT-qPCR. Fibronectin-1 (FN-1) and vinculin are two well-known proteins that promote cell adhesion ( Bays and DeMali, 2017 ; Sun et al, 2020 ; Yang et al, 2020 ). FN-1 and vinculin were upregulated in the D/PCL-2 scaffold ( Figure 8H ), which suggests that the D/PCL-2 scaffold promoted cell adhesion and proliferation by enhancing the two proteins.…”

Section: Resultsmentioning

confidence: 99%

Use of Electrospun Phenylalanine/Poly-ε-Caprolactone Chiral Hybrid Scaffolds to Promote Endothelial Remodeling

Sun

Hou

Sun

et al. 2021

Front. Bioeng. Biotechnol.

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The fabrication of tissue-engineered vascular grafts to replace damaged vessels is a promising therapy for cardiovascular diseases. Endothelial remodeling in the lumen of TEVGs is critical for successful revascularization. However, the construction of well-functioning TEVGs remains a fundamental challenge. Herein, chiral hybrid scaffolds were prepared by electrospinning using D/L-phenylalanine based gelators [D(L)PHEG] and poly-ε-caprolactone (PCL). The chirality of scaffolds significantly affected the endothelial remodeling progress of TEVGs. Compared with L-phenylalanine based gelators/poly-ε-caprolactone (L/PCL) and PCL, D-phenylalanine based gelators/poly-ε-caprolactone (D/PCL) scaffolds enhanced cell adhesion, and proliferation and upregulated the expression of fibronectin-1, and vinculin. These results suggests that chiral hybrid scaffolds can promote endothelial remodeling of TEVGs by upregulating adhesion-associated protein levels. This study offers an innovative strategy for endothelial remodeling of TEVGs by fabricating chiral hybrid scaffolds, and provides new insight for the treatment of cardiovascular diseases.

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“…Fibronectin is an important and ubiquitous component of the ECM with structural and functional roles in homeostasis and regeneration (To and Midwood, 2011). So far, fibronectin has also been found in the growth plate of bone and during new bone formation, which may promote osteoblast differentiation and the mineralization process (Yang et al, 2020). Although higher levels of fibronectin were found in osteoporotic Conversely, the stretched fibronectin conformation shows a higher binding affinity of IL-7.…”

Section: Discussionmentioning

confidence: 99%

“…patients, the downregulation of a fibronectin receptor (β 1integerin) on the cell surface reduced fibronectin mediated signaling in osteoblast and might explain the loss of bone despite higher fibronectin concentrations (Yang et al, 2020). Whether these higher fibronectin levels contribute to the osteoporotic pathology via immunomodulatory mechanisms, has not yet been fully elucidated.…”

Section: Discussionmentioning

confidence: 99%

Mechanobiological Principles Influence the Immune Response in Regeneration: Implications for Bone Healing

Knecht

Bucher

Linthout

et al. 2021

Front. Bioeng. Biotechnol.

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A misdirected or imbalanced local immune composition is often one of the reasons for unsuccessful regeneration resulting in scarring or fibrosis. Successful healing requires a balanced initiation and a timely down-regulation of the inflammation for the re-establishment of a biologically and mechanically homeostasis. While biomaterial-based approaches to control local immune responses are emerging as potential new treatment options, the extent to which biophysical material properties themselves play a role in modulating a local immune niche response has so far been considered only occasionally. The communication loop between extracellular matrix, non-hematopoietic cells, and immune cells seems to be specifically sensitive to mechanical cues and appears to play a role in the initiation and promotion of a local inflammatory setting. In this review, we focus on the crosstalk between ECM and its mechanical triggers and how they impact immune cells and non-hematopoietic cells and their crosstalk during tissue regeneration. We realized that especially mechanosensitive receptors such as TRPV4 and PIEZO1 and the mechanosensitive transcription factor YAP/TAZ are essential to regeneration in various organ settings. This indicates novel opportunities for therapeutic approaches to improve tissue regeneration, based on the immune-mechanical principles found in bone but also lung, heart, and skin.

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Fibronectin 1 activates WNT/β-catenin signaling to induce osteogenic differentiation via integrin β1 interaction

Cited by 38 publications

References 37 publications

Probiotic Propionibacterium freudenreichii MJ2 Enhances Osteoblast Differentiation and Mineralization by Increasing the OPG/RANKL Ratio

Probiotic Propionibacterium freudenreichii MJ2 Enhances Osteoblast Differentiation and Mineralization by Increasing the OPG/RANKL Ratio

Use of Electrospun Phenylalanine/Poly-ε-Caprolactone Chiral Hybrid Scaffolds to Promote Endothelial Remodeling

Mechanobiological Principles Influence the Immune Response in Regeneration: Implications for Bone Healing

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