Fibroblasts: The grey eminence of mammary gland development

Sumbal, Jakub; Belisova, Denisa; Koledová, Zuzana

doi:10.1016/j.semcdb.2020.10.012

Cited by 15 publications

(18 citation statements)

References 77 publications

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“…The mammary gland is comprised of two major components: mammary epithelium, which forms mammary ductal and lobular structures, and mammary stroma, which includes fibroblasts, adipocytes, preadipocytes, endothelial cells, and/or immune cells. Although the epithelium has been recognized to be an essential player in the mammary gland, the involvement of fibroblasts in mammary gland organization has also been extensively discussed ( Wiseman and Werb, 2002 ; Sumbal et al, 2021 ). For instance, fibroblasts are important for the production and remodeling of extracellular matrix (ECM).…”

Section: Introductionmentioning

confidence: 99%

“…While the population-specific roles of mammary gland fibroblasts in human breast tissues are just beginning to be unraveled ( Morsing et al, 2016 ; Morsing et al, 2020 ), the characterization of mouse mammary gland fibroblasts would help to deepen the understanding of the mammary gland in both humans and mice. The functional roles of mammary gland fibroblasts have been accumulated using mouse models ( Wiseman and Werb, 2002 ; Sumbal et al, 2021 ) and then extrapolated to human breast studies. Although the fibroblast populations in the mouse mammary gland have been shown to be heterogeneous ( Sumbal et al, 2021 ), their detailed properties and contributions to mammary gland organization remains inadequately defined.…”

Section: Introductionmentioning

confidence: 99%

“…The functional roles of mammary gland fibroblasts have been accumulated using mouse models ( Wiseman and Werb, 2002 ; Sumbal et al, 2021 ) and then extrapolated to human breast studies. Although the fibroblast populations in the mouse mammary gland have been shown to be heterogeneous ( Sumbal et al, 2021 ), their detailed properties and contributions to mammary gland organization remains inadequately defined.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Single-Cell Transcriptomics Identifies Heterogeneity of Mouse Mammary Gland Fibroblasts With Distinct Functions, Estrogen Responses, Differentiation Processes, and Crosstalks With Epithelium

Yoshitake

Chang

Saeki

et al. 2022

Front. Cell Dev. Biol.

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Fibroblasts have been shown to be one of the essential players for mammary gland organization. Here, we identify two major types of mouse mammary gland fibroblasts through single-cell RNA sequencing analysis: Dpp4+ fibroblasts and Dpp4- fibroblasts. Each population exhibits unique functional characteristics as well as discrete localization in normal mouse mammary glands. Remarkably, estrogen, a crucial mediator of mammary gland organization, alters the gene expression profiles of fibroblasts in a population-specific manner, without distinct activation of estrogen receptor signaling. Further integrative analysis with the inclusion of five other publicly available datasets reveals a directional differentiation among the mammary gland fibroblast populations. Moreover, the combination with the mouse mammary epithelium atlas allows us to infer multiple potential interactions between epithelial cells and fibroblasts in mammary glands. This study provides a comprehensive view of mouse mammary gland fibroblasts at the single-cell level.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Single-Cell Transcriptomics Identifies Heterogeneity of Mouse Mammary Gland Fibroblasts With Distinct Functions, Estrogen Responses, Differentiation Processes, and Crosstalks With Epithelium

Yoshitake

Chang

Saeki

et al. 2022

Front. Cell Dev. Biol.

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“…Zuzana Koledova from Masaryk University in Czech Republic closed this session with a short talk on her current work on the role of fibroblasts on mammary gland morphogenesis. Fibroblasts are known to interact with the mammary gland epithelium through paracrine signaling as well as through extracellular matrix production and remodeling [25]. Zuzana Koledova team currently investigates the potential involvement of mechanical forces exerted by fibroblasts in mammary epithelial branching using mouse models, co-cultures of fibroblast and mammary gland epithelium [26] and time lapse visualization of organoid dynamics and branching.…”

Section: Meeting Reportmentioning

confidence: 99%

Twelfth Annual ENBDC Workshop: Methods in Mammary Gland Biology and Breast Cancer

Charifou

Traustadóttir

Bentires‐Alj

et al. 2021

J Mammary Gland Biol Neoplasia

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The twelfth annual workshop of the European Network for Breast Development and Cancer focused on methods in mammary gland biology and breast cancer, was scheduled to take place on March 26–28, 2020, in Weggis, Switzerland. Due to the COVID-19 pandemic, the meeting was rescheduled twice and eventually happened as a virtual meeting on April 22 and 23, 2021. The main topics of the meeting were branching and development of the mammary gland, tumor microenvironment, circulating tumor cells, tumor dormancy and breast cancer metastasis. Novel and unpublished findings related to these topics were presented, with a particular focus on the methods used to obtain them. Virtual poster sessions were a success, with many constructive and fruitful interactions between researchers and covered many areas of mammary gland biology and breast cancer.

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“…They are recruited to radiation-damaged sites, activated to myofibroblasts, and lay down new extracellular matrix (ECM) to promote healing 23 . Fibroblasts are found within the murine mammary gland and, at even higher amounts, in human breast tissue 24,25 . Therefore, fibroblasts within the mammary tissue will be exposed to off-target effects of RT and may interact with residual or recruited CTCs to influence recurrence.…”

Section: Introductionmentioning

confidence: 99%

Radiation-Induced Metabolic Reprogramming of Fibroblasts Regulates the Breast Cancer Microenvironment

Corn

Britto

Ivanova

et al. 2022

Preprint

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Triple negative breast cancer (TNBC) patients continue to have high recurrence rates despite current treatment modalities, including radiation therapy (RT). Radiation-resistant TNBC cells and circulating tumor cells thought to be involved in recurrence survive in part due to changes in their metabolic profiles. These tumor cells interact with radiation-damaged stromal cells such as fibroblasts following treatment. How fibroblasts metabolically respond to RT and metabolically crosstalk with TNBC cells is poorly understood. In this study, we identified that radiation-damaged fibroblasts accumulate lipids up to 7-days following treatment due to increased autophagic flux. This lipid accumulation and autophagy allows fibroblasts to maintain increased fatty acid oxidation, overall mitochondrial respiration, and aerobic glycolysis rates. TNBC cells responded by decreasing autophagy and increasing ATP-linked respiration and migration rates. Our work highlights how metabolic crosstalk between radiation-damaged fibroblasts and TNBC cells leads to a microenvironment conducive to recurrence.

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Fibroblasts: The grey eminence of mammary gland development

Cited by 15 publications

References 77 publications

Single-Cell Transcriptomics Identifies Heterogeneity of Mouse Mammary Gland Fibroblasts With Distinct Functions, Estrogen Responses, Differentiation Processes, and Crosstalks With Epithelium

Single-Cell Transcriptomics Identifies Heterogeneity of Mouse Mammary Gland Fibroblasts With Distinct Functions, Estrogen Responses, Differentiation Processes, and Crosstalks With Epithelium

Twelfth Annual ENBDC Workshop: Methods in Mammary Gland Biology and Breast Cancer

Radiation-Induced Metabolic Reprogramming of Fibroblasts Regulates the Breast Cancer Microenvironment

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