Fibroblast populated dense collagen matrices: cell migration, cell density and metalloproteinases expression

Concentrated collagen hydrogels as dermal substitutes

“…The concentration process was carried out by controlled evaporation of the solvent in sterile conditions [11]. The collagen concentration was then estimated by hydroxyproline titration [26].…”

“…The contacts between Integra structure and cells are in two dimensions and can be considered as non physiological [10]. More recently, another acellular scaffold, a dense collagen matrix concentrated at 40 mg/ml has been developed with interesting mechanical properties [11][12][13]. In vitro, human dermal fibroblasts migrate into the dense collagen network to reach a distance of about 350 mm after 28 days.…”

Silica–collagen bionanocomposites as three-dimensional scaffolds for fibroblast immobilization

“…Gelatin zymography was carried out using the Miniprotean III system (Bio-Rad), using a previously reported procedure [26]. Polyacrylamide migration gel was performed at 10% (ratio acrylamide/ bis-acrylamide 37:1) in 0.375 M Tris-HCl, pH 8.8, containing 1 mg ml -1 gelatin and 0.1% sodium dodecyl sulfate (SDS).…”

Collagen matrix as a tool in studying fibroblastic cell behavior

“…They reach a density similar to that found in human dermis. 36,37 The differences between cell growth on hard plastic surface and on soft collagen gel suggest that the rigidity of the substrate plays a crucial role. Cells can be placed between 2 sheets of collagen-coated polyacrylamide gel.…”