Fibroblast Growth Factor Receptor (FGFR)-1 and -2 in the Ovine Corpus Luteum throughout the Estrous Cycle

Doraiswamy, Vina Yak; Knutson, Darlene L.; Grazul-Bilska, Anna T.; Redmer, Dale A.; Reynolds, Lawrence P.

doi:10.3109/08977199809002123

Cited by 25 publications

(12 citation statements)

References 49 publications

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“…Detection of factor VIII, VEGF, VEGFR1, FGF2, and FGFR protein was performed as previously described [49][50][51]. Briefly, sections of placentomes fixed in formalin or Carnoy's solution were rinsed several times in PBS containing Triton-X100 (0.3%, v/v) and then were treated for 20 min with blocking buffer [PBS containing normal goat serum (1-2%, v/v)] followed by incubation with specific primary antibody overnight at 4°C.…”

Section: Immunohistochemistrymentioning

confidence: 99%

Placental vascularity and growth factor expression in singleton, twin, and triplet pregnancies in the sheep

Vonnahme

Evoniuk

Johnson

et al. 2008

Endocr

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For singleton, twin, and triplet pregnancies, uteri were collected on day 140 of pregnancy. For each ewe (n = 18), placentomes were fixed by arterial perfusion supplying the fetal (cotyledon) and maternal placenta (caruncle). Tissue sections were stained for determination of vascularity by image analysis. Further, protein expression for factor VIII, vascular endothelial growth factor (VEGF) and its receptor, VEGFR1, as well as basic fibroblast growth factor (FGF2) and its receptor, FGFR, in tissue sections was determined by immunohistochemistry and image analyses. Cotyledonary and caruncular samples were analyzed for expression of mRNA for Vegf and its two receptors, Vegfr1 and Vegfr2, as well as Fgf2 and Fgfr. Fetal number did not affect placental capillary density or factor VIII expression, whereas increased fetal number reduced total cotyledon and caruncle capillary volume. While expression of Vegf, Vegfr1, Vegfr2, and Fgfr mRNA in cotyledonary but not caruncular tissue was greater in twin pregnancies compared to singleton and triplet pregnancies, protein expression of VEGF in the placentome decreased with increasing numbers of fetuses, VEGFR1 did not change, and FGFR was greater in twin versus singleton and triplet pregnancies. Fetal number did not affect the expression of Fgf2 mRNA in placental tissues, whereas FGF2 protein expression was less in triplet compared to singleton and twin pregnancies. Reduced fetal and placental weights in twins and/or triplet pregnancies are associated with an overall decrease in total placental vascularity, VEGF and FGF2 and/or FGFR protein expression, but not in angiogenic factor mRNA expression or VEGFR1 protein expression in sheep.

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Section: Immunohistochemistrymentioning

confidence: 99%

Placental vascularity and growth factor expression in singleton, twin, and triplet pregnancies in the sheep

Vonnahme

Evoniuk

Johnson

et al. 2008

Endocr

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“…Immunohistochemistry and topical autoradiography demonstrate that both parenchymal and nonparenchymal (endothelial, fibroblastic) cells express FGF-1 and FGF-2. The distribution suggest that FGF is involved in the regulation of luteal parenchymal and vascular function [54]. Another member of FGF family, keratinocyte growth factor (FGF-7) mRNA expression was recently demonstrated in bovine CL and the protein is primarily localized in small luteal cells.…”

Section: Fgf In Bovine Corpus Luteummentioning

confidence: 99%

Angiogenic Factors (VEGF, FGF and IGF) in the Bovine Corpus Luteum.

Schams

Berisha

2002

Journal of Reproduction and Development

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Abstract. Angiogenesis, the development of the new capillaries by endothelial cell proliferation and outgrowth from pre-existing vessels, is one of the prominent features of early corpus luteum (CL). The process of angiogenesis is an important component of normal development and function of CL. Of the numerous promoters of angiogenesis and maintenance of new established capillaries that have been identified, the most important factors appear to be vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (FGF). The biological effects of VEGF and FGF factor families are mediated by signaling through specific tyrosine kinase receptors. The VEGF, FGF and IGF family members in bovine CL are discussed with the literature available for large domestic animals. The highest expression for VEGF, VEGFR-2, FGF-2, FGFR, IGF-1, IGF-2 and IGFR1 were found during the early luteal phase (Ld 1-4) followed by a significant decrease afterwards. The VEGF and IGF-1 protein was localized predominantly in luteal cells. FGF-1 mRNA expression peaked (P<0.05) during mid-luteal stage and FGF-1 protein was localized in cytoplasm of luteal cells, but endothelial cells are always negative. FGF-2 protein during angiogenesis (d 1-5) was found in cytoplasm of endothelial cells and changed thereafter exclusively to the cytoplasm of luteal cells. In contrast, the IGF-2 protein is exclusively localized in pericytes and few endothelial cells. The results obtained (mainly in ruminant) suggest an important role of these growth factors for angiogenesis and furthermore for maintenance and function of the bovine corpus luteum.

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“…Approximately 50% of the cells of the mature CL are endothelial cells [14,15]. The vascular development of the CL is regulated by angiogenic factors, including VEGF [14][15][16][17][18], angiopoetins (ANGP1, ANGP2) [14,[18][19][20], and FGF2 [21][22][23][24][25]. FGF2 mRNA synthesis is stimulated by LH in bovine CL and in cultured luteal cells [22].…”

Section: Corpus Luteummentioning

confidence: 97%

“…FGF2 mRNA synthesis is stimulated by LH in bovine CL and in cultured luteal cells [22]. During human CL formation, FGF2 is localized in granulosa, and theca lutein cells [23], FGF receptors (FGFR1 and 2), were demonstrated in the parenchyma as well as the vasculature of the CL [24]. During luteolysis, the regressing CL is characterized over a few days by a rapid phase of tissue disintegration and over several weeks by a slow phase of tissue remodeling, macrophage infiltration, and fibroblastic growth.…”

Section: Corpus Luteummentioning

confidence: 98%

“…Fractions (0.2 ml) were eluted with PBS and their radioactivity counted in a ␤-scintillation counter. Degradation fragments of HS side chains are eluted at 0.5 Ͻ K av Ͻ 0.8 (fractions [12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30]. Nearly intact HSPGs are eluted just after the Vo (K av Ͻ 0.2, fractions 2-10).…”

Section: Heparanase Activity Assaymentioning

confidence: 99%

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Spatiotemporal Expression of Heparanase During Human and Rodent Ovarian Folliculogenesis1

Haimov‐Kochman

Prus

Zcharia

et al. 2005

Biology of Reproduction

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Heparanase (HPSE) is an endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs), major components of the basement membrane (BM) and extracellular matrix (ECM). Heparanase activity results in release of HSPG-bound molecules, including basic fibroblast growth factor (FGF2). Structural and functional development of the corpus luteum (CL) involves tissue remodeling, active angiogenesis, and steroid production. Heparanase-induced ECM and BM breakdown as well as FGF2-stimulated endothelial proliferation may have an important role in the regulation of luteal function. Heparanase mRNA was detected by reverse-transcription-polymerase chain reaction in granulosa cells recovered from follicular fluid of in vitro fertilization patients. Using sulfate-labeled ECM, heparanase enzymatic activity was determined in human luteinized granulosa cells. Employing immunohistochemistry, heparanase protein was localized predominantly in the theca interna cell layer of the mature antral follicle, whereas in human corpora lutea, both luteinized granulosa and theca cells were immunostained for heparanase. During luteolysis, heparanase was identified in macrophages surrounding the forming corpus albicans. In serially sectioned ovaries from unstimulated rats as well as from eCG-treated rats, expression of heparanase was noted exclusively in the ovarian steroid-producing interstitial tissue. Following an ovulatory dose of hCG, heparanase was immunostained also in lutein cells of the forming corpora lutea. Temporal expression of heparanase in granulosa cells during the luteal phase and in macrophages during luteal regression supports the hypothesis that heparanase plays a role in human ovarian ECM remodeling and may potentiate cellular migration and growth factor bioavailability.

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Fibroblast Growth Factor Receptor (FGFR)-1 and -2 in the Ovine Corpus Luteum throughout the Estrous Cycle

Cited by 25 publications

References 49 publications

Placental vascularity and growth factor expression in singleton, twin, and triplet pregnancies in the sheep

Placental vascularity and growth factor expression in singleton, twin, and triplet pregnancies in the sheep

Angiogenic Factors (VEGF, FGF and IGF) in the Bovine Corpus Luteum.

Spatiotemporal Expression of Heparanase During Human and Rodent Ovarian Folliculogenesis1

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