Fibroblast growth factor (FGF) 2 and FGF9 mediate mesenchymal–epithelial interactions of peritubular and Sertoli cells in the rat testis

Ramy, Rosy El; Verot, Adélie; Mazaud, Séverine; Odet, Fanny; Magre, Solange; Magueresse-Battistoni, Brigitte Le

doi:10.1677/joe.1.06146

Cited by 41 publications

(20 citation statements)

References 61 publications

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“…In rats, the FGF2 receptor has been traced to several somatic cell types (Sertoli cells, Leydig cells, and myoid peritubular cells; Le Magueresse-Battistoni et al 1994, El Ramy et al 2005. Indeed, FGF2 has been associated with promoting both the proliferation and survival of somatic cells in vitro (Han et al 1993, Van Dissel-Emiliani et al 1996, Steger et al 1998.…”

Section: Discussionmentioning

confidence: 99%

“…The somatic cell component of the cell suspensions will require further investigation as these cells possess receptors for the growth factors used (Le Magueresse-Battistoni et al 1994, Wahab-Wahlgren et al 2003, Dorval-Coiffec et al 2005, El Ramy et al 2005, Kassab et al 2007). It will be interesting to study the prevalence of the different somatic cell types under the action of the different growth factors and the effect of specific somatic cell types on the homing process of bovine SSCs in the mouse seminiferous tubule environment.…”

Section: Discussionmentioning

confidence: 99%

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Propagation of bovine spermatogonial stem cells in vitro

Aponte¹,

Soda²,

Teerds³

et al. 2008

Reproduction

138

113

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The access to sufficient numbers of spermatogonial stem cells (SSCs) is a prerequisite for the study of their regulation and further biomanipulation. A specialized medium and several growth factors were tested to study the in vitro behavior of bovine type A spermatogonia, a cell population that includes the SSCs and can be specifically stained for the lectin Dolichos biflorus agglutinin. During short-term culture (2 weeks), colonies appeared, the morphology of which varied with the specific growth factor(s) added. Whenever the stem cell medium was used, round structures reminiscent of sectioned seminiferous tubules appeared in the core of the colonies. Remarkably, these round structures always contained type A spermatogonia. When leukemia inhibitory factor (LIF), epidermal growth factor (EGF), or fibroblast growth factor 2 (FGF2) were added, specific effects on the numbers and arrangement of somatic cells were observed. However, the number of type A spermatogonia was significantly higher in cultures to which glial cell line-derived neurotrophic factor (GDNF) was added and highest when GDNF, LIF, EGF, and FGF2 were all present. The latter suggests that a proper stimulation of the somatic cells is necessary for optimal stimulation of the germ cells in culture. Somatic cells present in the colonies included Sertoli cells, peritubular myoid cells, and a few Leydig cells. A transplantation experiment, using nude mice, showed the presence of SSCs among the cultured cells and in addition strongly suggested a more than 10 000-fold increase in the number of SSCs after 30 days of culture. These results demonstrate that bovine SSC self-renew in our specialized bovine culture system and that this system can be used for the propagation of these cells.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Propagation of bovine spermatogonial stem cells in vitro

Aponte¹,

Soda²,

Teerds³

et al. 2008

Reproduction

138

113

View full text Add to dashboard Cite

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“…In other systems, FGF-2 is able to induce gelatinase expression (Kohn et al, 1995;El Ramy et al, 2005;Wang et al, 2005), suggestive of a positive feedback loop between growth factor release and the expression/activation of MMPs to support lens epithelial cell survival and maintain proliferation. Our data have also demonstrated how important the physical contact with the lens capsule is to enhance the effects of the released growth factors (Figure 4).…”

Section: The Lens Capsule Generates a Microenvironment Conducive To Ementioning

confidence: 99%

FGF-2 Release from the Lens Capsule by MMP-2 Maintains Lens Epithelial Cell Viability

Tholozan

Gribbon

et al. 2007

MBoC

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The lens is an avascular tissue, separated from the aqueous and vitreous humors by its own extracellular matrix, the lens capsule. Here we demonstrate that the lens capsule is a source of essential survival factors for lens epithelial cells. Primary and immortalized lens epithelial cells survive in low levels of serum and are resistant to staurosporine-induced apoptosis when they remain in contact with the lens capsule. Physical contact with the capsule is required for maximal resistance to stress. The lens capsule is also a source of soluble factors including fibroblast growth factor 2 (FGF-2) and perlecan, an extracellular matrix component that enhances FGF-2 activity. Matrix metalloproteinase 2 (MMP-2) inhibition as well as MMP-2 pretreatment of lens capsules greatly reduced the protective effect of the lens capsule, although this could be largely reversed by the addition of either conditioned medium or recombinant FGF-2. These data suggest that FGF-2 release from the lens capsule by MMP-2 is essential to lens epithelial cell viability and survival. INTRODUCTIONCell survival requires the continued exposure to specific survival factors. In a classic study (Ishizaki et al., 1993), lens epithelial cells were shown to survive in culture, in the absence of cell-cell contact, as long as the right growth factor cues were available. These factors could be provided exogenously either in the form of conditioned media or in serum supplement (Ishizaki et al., 1993), leading to the widely accepted general hypothesis that cells are programmed to die unless the appropriate signals are received.The recent proposal of the extracellular matrix (ECM) reservoir hypothesis suggests that the ECM itself can act as a reservoir for growth and survival factors (Bergers et al., 2000;Mott and Werb, 2004) that are released via the action of various matrix metalloproteinases (MMPs; reviewed in McCawley and Matrisian, 2001;Mott and Werb, 2004). MMPs have been shown to release fibroblast growth factor (FGF)-2 (MMP-1, -3; Whitelock et al., 1996) and fibroblast growth factor receptor (FGFR)-1 (MMP-2; Levi et al., 1996), activate transforming growth factor (TGF)-␤1/2 (Yu and Stamenkovic, 2000), and to both activate and release insulin-like growth factor (IGF)-1 (MMP-1, -2, -3, and -9;Fowlkes et al., 1994; and MMP-2, -3, and -7;Imai et al., 1997). MMPs are therefore an essential component in this proposed role for the ECM in cell survival and cell proliferation (Ishizuya-Oka et al., 2000;Wiseman et al., 2003). From such data, we can see that the ECM reservoir hypothesis requires three essential components: an ECM, growth factors sequestered by the ECM, and the presence of MMPs to release the growth factors from the ECM. This hypothesis changes our perception of the ECM as it can no longer be considered to solely provide a physical support or appropriate cues for integrin signaling (Hynes, 1992;Adams and Watt, 1993), but is also a source of cell proliferation and survival factors (Klagsbrun, 1990;Taipale and Keski-Oja, 1997;Bergers et al., 2000;...

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“…[5][6][7][8][9][10][11] Furthermore, new data suggest that chemokines and MMPs may be working together in the regulation of stem cell recruitment and migration. 12,13 Thus, it appears that in Sertoli cells, ERM is responsible for maintaining the production of factors common to other organs and tissues, which may help to retain stem cells inside their niches and balance their self-renewal and differentiation pathways. With ERM inactivation, spermatogonial stem cells are not maintained and differentiation is facilitated ( Fig.…”

Section: Erm Is Essential For Maintenance Of the Adult Spermatogonialmentioning

confidence: 99%

Mechanistic Insights into the Regulation of the Spermatogonial Stem Cell Niche

Hess¹,

Cooke²,

Hofmann³

et al. 2006

Cell Cycle

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Potential therapeutic use of stem cells in the treatment of human diseases depends on our ability to control the balance of their differentiation and self-renewal in vitro and in vivo. The stem cell "niche," or specialized microenvironment, is now recognized as one of the major contributors to this regulation in many species. Our recent study, which was reported in Nature, was the first to demonstrate that expression of a vertebrate animal transcription factor is essential for the maintenance of a stem cell niche. In that letter, targeted disruption of ERM (Ets-related molecule), which was localized only in the somatic support cell of the testis, the Sertoli cell, resulted in failure of self-renewal by spermatogonial stem cells, following the first wave of spermatogenesis. One of the more important conclusions drawn was the realization that regulation of the stem cell niche during the perinatal period, a phase characterized by rapid mitosis of both spermatogonial stem cells and Sertoli cells, differed from that in the adult. It appears that the ERM-regulated pathways are coincident with the termination of Sertoli cell proliferation and commencement of the cycle of spermatogenesis, which is sustained by the same cell that regulates the stem cell niche. Several likely targets for ERM regulation are discussed, as well as their potential implications for increasing our understanding of spermatogonial stem cell activity and the uniqueness of the Sertoli cell's immune privilege and possible utility for the protection of transplanted adult stem cells.

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Fibroblast growth factor (FGF) 2 and FGF9 mediate mesenchymal–epithelial interactions of peritubular and Sertoli cells in the rat testis

Cited by 41 publications

References 61 publications

Propagation of bovine spermatogonial stem cells in vitro

Propagation of bovine spermatogonial stem cells in vitro

FGF-2 Release from the Lens Capsule by MMP-2 Maintains Lens Epithelial Cell Viability

Mechanistic Insights into the Regulation of the Spermatogonial Stem Cell Niche

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