Fibroblast growth factor and vascular endothelial growth factor play a critical role in endotheliogenesis from human adipose-derived stem cells

Khan, Samina; Villalobos, Miguel; Choron, Rachel L.; Chang, Shaohua; Brown, Spencer A.; Carpenter, Jeffrey P.; Tulenko, Thomas N.; Zhang, Ping

doi:10.1016/j.jvs.2016.04.034

Cited by 59 publications

(54 citation statements)

References 39 publications

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“…Our research emphasis was that the underlying role of VEGF‐Ang‐1‐PLA nano‐sustained release microspheres on ADSC proliferation and differentiation toward ECs. It was reported that pre‐culture in endothelial growth medium enhanced the angiogenic properties of ADSCs (Souza et al, ), and Sophia Khan found that the two vital angiogenesis factors FGF and VEGF played critical roles in endotheliogenesis from human ADSCs (Khan et al, ). The present study VEGF‐Ang‐1‐PLA microspheres facilitated HGF secretion to promote ADSC proliferation and enhance CD31 + cells, but scarcely influenced the expression levels of VEGF and Ang‐1.…”

Section: Discussionmentioning

confidence: 99%

Effects of VEGF‐ANG‐1‐PLA nano‐sustained release microspheres on proliferation and differentiation of ADSCs

Chen

et al. 2018

Cell Biology International

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The improvement of fat graft viability might depend on the presence of multipotent resident adipose derived stem cells (ADSCs) which is the important component of stromal vascular fraction (SVF). Vascular endothelial growth factor (VEGF) and angiogenin-1 (Ang-1) are responsible for neovascularization. However, their half-life is too short to produce a biological effect. We thus investigated whether VEGF-ANG-1-polylactic acid (PLA) microspheres could enhance the angiogenic properties of ADSCs. PLA microspheres containing VEGF and ANG-1 were prepared by in vitro ultrasonic emulsification and characterized according to their encapsulation efficiency (EE), drug-loading rate (DL), particle size, and drug release. The systemic toxicity of empty loaded nanospheres (NPs) and the ability of these microspheres to promote the proliferation and differentiation of ADSCs were evaluated. The EE and DL were above 86 and 2.8%, respectively. The drug release was completed after 20 days. Systemic toxicity was verified in ADSCs that received the unloaded NPs. It was observed that ADSCs treated with VEGF-ANG-1-PLA microspheres had an increase in the proliferation and the number of CD31 positive cells. ADSCs proliferation and differentiation toward endothelial cells (ECs) could be enhanced by the addition of VEGF-ANG-1-PLA nano-sustained release microspheres.

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Section: Discussionmentioning

confidence: 99%

Effects of VEGF‐ANG‐1‐PLA nano‐sustained release microspheres on proliferation and differentiation of ADSCs

Chen

et al. 2018

Cell Biology International

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“…The endothelial differentiation of ASCs was induced using EGM2, as commonly performed . This media is supplemented with factors implicated in the differentiation and survival of ECs, including vascular endothelial growth factor and basic fibroblast growth factor .…”

Section: Resultsmentioning

confidence: 99%

“…The failure of previous investigations to detect the limited endothelial plasticity of ASCs may be largely attributed to their use of qualitative assays and their omission of representative primary cell controls in evaluating an endothelial phenotype. The expression of endothelial genes by ASCs has often been evaluated by end‐point PCR , and that of proteins by Western blots , immunofluorescence microscopy , and flow cytometry . Similarly, AcLDL uptake has predominantly been assessed by immunofluorescence microscopy .…”

Section: Discussionmentioning

confidence: 99%

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Limited Endothelial Plasticity of Mesenchymal Stem Cells Revealed by Quantitative Phenotypic Comparisons to Representative Endothelial Cell Controls

Antonyshyn

McFadden

Gramolini

et al. 2018

Stem Cells Translational Medicine

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Considerable effort has been directed toward deriving endothelial cells (ECs) from adipose‐derived mesenchymal stem cells (ASCs) since 2004, when it was first suggested that ECs and adipocytes share a common progenitor. While the capacity of ASCs to express endothelial markers has been repeatedly demonstrated, none constitute conclusive evidence of an endothelial phenotype as all reported markers have been detected in other, non‐endothelial cell types. In this study, quantitative phenotypic comparisons to representative EC controls were used to determine the extent of endothelial differentiation being achieved with ASCs. ASCs were harvested from human subcutaneous abdominal white adipose tissue, and their endothelial differentiation was induced using well‐established biochemical stimuli. Reverse transcription quantitative real‐time polymerase chain reaction and parallel reaction monitoring mass spectrometry were used to quantify their expression of endothelial genes and corresponding proteins, respectively. Flow cytometry was used to quantitatively assess their uptake of acetylated low‐density lipoprotein (AcLDL). Human umbilical vein, coronary artery, and dermal microvascular ECs were used as positive controls to reflect the phenotypic heterogeneity between ECs derived from different vascular beds. Biochemically conditioned ASCs were found to upregulate their expression of endothelial genes and proteins, as well as AcLDL uptake, but their abundance remained orders of magnitude lower than that observed in the EC controls despite their global proteomic heterogeneity. The findings of this investigation demonstrate the strikingly limited extent of endothelial differentiation being achieved with ASCs using well‐established biochemical stimuli, and underscore the importance of quantitative phenotypic comparisons to representative primary cell controls in studies of differentiation. Stem Cells Translational Medicine 2019;8:35–45

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“…* Table includes data from the following references [2,18,26,27,28,29,30,31,32,33,34,35,36]. Abbreviations: CXCL12, C-X-C motif chemokine ligand 12; EGF, endothelial growth factor; FGF, fibroblast growth factor; IL-10, interleukin-10; M-CSF, monocyte colony-stimulating factor; NO, nitric oxide; PDGF, platelet derived growth factor; TNF-α, tumor necrosis factor-α; VEGF, vascular endothelial growth factor.…”

Section: Tablementioning

confidence: 99%

Minimizing Skin Scarring through Biomaterial Design

Moore

Longaker

2017

JFB

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Wound healing continues to be a major burden to patients, though research in the field has expanded significantly. Due to an aging population and increasing comorbid conditions, the cost of chronic wounds is expected to increase for patients and the U.S. healthcare system alike. With this knowledge, the number of engineered products to facilitate wound healing has also increased dramatically, with some already in clinical use. In this review, the major biomaterials used to facilitate skin wound healing will be examined, with particular attention allocated to the science behind their development. Experimental therapies will also be evaluated.

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Fibroblast growth factor and vascular endothelial growth factor play a critical role in endotheliogenesis from human adipose-derived stem cells

Cited by 59 publications

References 39 publications

Effects of VEGF‐ANG‐1‐PLA nano‐sustained release microspheres on proliferation and differentiation of ADSCs

Effects of VEGF‐ANG‐1‐PLA nano‐sustained release microspheres on proliferation and differentiation of ADSCs

Limited Endothelial Plasticity of Mesenchymal Stem Cells Revealed by Quantitative Phenotypic Comparisons to Representative Endothelial Cell Controls

Minimizing Skin Scarring through Biomaterial Design

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