Fibroblast Growth Factor–2 Regulates the Cell Function of Human Dental Pulp Cells

Shimabukuro, Yoshio; Ueda, Masaru; Ozasa, Masao; Anzai, Jun; Takedachi, Masahide; Yanagita, Manabu; Ito, Masako; Hashikawa, Tomoko; Yamada, Satoru; Murakami, Shinya

doi:10.1016/j.joen.2009.08.010

Cited by 67 publications

(65 citation statements)

References 47 publications

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“…These findings suggest that FGF-2 derived from injured dentin can play a pivotal role in wound healing and dentin/pulp complex regeneration. However, the effects of FGF-2 were reported in MSCs, DPSCs, and HDPCs (6,7,(9)(10)(11)(12)(13)15); the precise mechanism of FGF-2's effects on HDPCs are not completely understood.…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, FGF-2 has been associated with tooth morphogenesis by contributing to the mineralization of the dentin matrix (8). FGF-2 can promote growth of dental pulp stem cells (DPSCs) and activate growth and migration of HDPCs, whereas it did not induce odontoblastic differentiation in human DPSCs (9) and HDPCs (10). In contrast, FGF-2 up-regulates ALP activity, the formation of mineralized nodules, the expression of dentin sialoprotein (DSP) and DMP-1 in human DPSC (11), DSP expression in adult rat incisor dental pulp cells (12), and hyaluronan in HDPCs (13).…”

mentioning

confidence: 99%

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Effects of Fibroblast Growth Factor-2 on the Expression and Regulation of Chemokines in Human Dental Pulp Cells

Kim

Min

Jeong

et al. 2010

Journal of Endodontics

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Effects of Fibroblast Growth Factor-2 on the Expression and Regulation of Chemokines in Human Dental Pulp Cells

Kim

Min

Jeong

et al. 2010

Journal of Endodontics

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“…Human PDL (HPDL) cells, dental pulp (HDP) cells, gingival fibroblasts (HGFs), and gingival epithelial cells (HGEs) were cultured according to previously described methods (Murakami et al, 2001(Murakami et al, , 2002Shimabukuro et al, 2005Shimabukuro et al, , 2009). We previously established a mouse PDL cell line, MPDL22, and used this as described in our earlier publication (Yamada et al, 2007).…”

Section: Cell Culture and Induction Of Pdl Cell Cytodifferentiationmentioning

confidence: 99%

Characterization of a Novel Periodontal Ligament-specific Periostin Isoform

et al. 2014

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Periostin is a mesenchymal cell marker predominantly expressed in collagen-rich fibrous connective tissues, including heart valves, tendons, perichondrium, periosteum, and periodontal ligament (PDL). Knockdown of periostin expression in mice results in early-onset periodontitis and failure of cardiac healing after acute myocardial infarction, suggesting that periostin is essential for connective tissue homeostasis and regeneration. However, its role(s) in periodontal tissues has not yet been fully defined. In this study, we describe a novel human isoform of periostin (PDL-POSTN). Isoform-specific analysis by reversetranscription polymerase chain-reaction (RT-PCR) revealed that PDL-POSTN was predominantly expressed in the PDL, with much lower expression in other tissues and organs. A PDL cell line transfected with PDL-POSTN showed enhanced alkaline phosphatase (ALPase) activity and calcified nodule formation, compared with cells transfected with the full-length periostin isoform. A neutralizing antibody against integrin-αv inhibited both ALPase activity and calcified nodule formation in cells transfected with PDL-POSTN. Furthermore, co-immunoprecipitation assays revealed that PDL-POSTN bound to integrin αvβ3 more strongly than the common isoform of periostin, resulting in strong activation of the integrin αvβ3-focal adhesion kinase (FAK) signaling pathway. These results suggest that PDL-POSTN positively regulates cytodifferentiation and mineralization in PDL cells through integrin αvβ3.

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“…In this study the inflammation observed after pulp capping was compatible with normal tissue repair processes and dentin tissue formation was evident. Collagen synthesis by fibroblasts during the inflammatory process is a key event for human pulp repair [27]. Frequently, the importance of inflammation in pulp healing has been underestimated, considered to be an undesirable effect leading in most cases to pulp necrosis.…”

Section: Discussionmentioning

confidence: 99%

In vitro and in vivo evaluation of the biocompatibility of a calcium phosphate/poly(lactic-co-glycolic acid) composite

Gala-García

Carneiro

Silva

et al. 2012

J Mater Sci: Mater Med

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This study assess the effects of bioceramic and poly(lactic-co-glycolic acid) composite (BCP/PLGA) on the viability of cultured macrophages and human dental pulp fibroblasts, and we sought to elucidate the temporal profile of the reaction of pulp capping with a composite of bioceramic of calcium phosphate and biodegradable polymer in the progression of delayed dentine bridge after (30 and 60 days) in vivo. Histological evaluation of inflammatory infiltrate and dentin bridge formation were performed after 30 and 60 days. There was similar progressive fibroblast growth in all groups and the macrophages showed viability. The in vivo study showed that of the three experimental groups: BCP/PLGA composite, BCP and calcium hydroxide (Ca(OH)(2)) dentin bridging was the most prevalent (90 %) in the BCP/PLGA composite after 30 days, mild to moderate inflammatory response was present throughout the pulp after 30 days. After 60 days was observed dentine bridging in 60 % and necrosis in 40 %, in both groups. The results indicate that understanding BCP/PLGA composite is biocompatible and by the best tissue response as compared to calcium hydroxide in direct pulp capping may be important in the mechanism of delayed dentine bridge after 30 and 60 days.

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Fibroblast Growth Factor–2 Regulates the Cell Function of Human Dental Pulp Cells

Cited by 67 publications

References 47 publications

Effects of Fibroblast Growth Factor-2 on the Expression and Regulation of Chemokines in Human Dental Pulp Cells

Effects of Fibroblast Growth Factor-2 on the Expression and Regulation of Chemokines in Human Dental Pulp Cells

Characterization of a Novel Periodontal Ligament-specific Periostin Isoform

In vitro and in vivo evaluation of the biocompatibility of a calcium phosphate/poly(lactic-co-glycolic acid) composite

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