Fibroblast growth factor 2 regulates cumulus differentiation under the control of the oocyte

Barros, Rodrigo Garcia; Lima, P. F.; Soares, Ana Caroline Silva; Sanches, L.; Price, Christopher A.; Buratini, J.

doi:10.1007/s10815-019-01436-7

Cited by 21 publications

(8 citation statements)

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“…These results agree with previous literature indicating that FGF2 added to regular IVM medium (carrying hormones) improves the degree of cumulus expansion and nuclear maturation [ 48 , 49 ]. Moreover, Barros et al [ 50 ] observed that FGF2 enhanced meiotic resumption, decreased cumulus cell dissociation, and inhibited the apoptosis on cumulus cells. However, Kobayashi et al [ 35 ] reported deficient cleavage rate after supplementation of the MM with FGF2 when the medium was lacking hormones.…”

Section: Discussionmentioning

confidence: 99%

Simple and Efficient Chemically Defined In Vitro Maturation and Embryo Culture System for Bovine Embryos

Arias

Vargas

Gallardo

et al. 2022

Animals

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Supplementation of the culture media for in vitro production (IVP) of bovine embryos with fetal bovine serum (FBS) is associated with inconsistent outcomes. The present study sought to replace FBS and BSA by insulin-like growth factor 1 (IGF1), fibroblast growth factor 2 (FGF2) and epidermal growth factor (EGF). In Experiment 1, absence of FBS from maturation medium (MM) did not affect the rate of in vitro maturation, as assessed by the extrusion of the first polar body. However, when gonadotropins and FBS were removed from the MM, the maturation rate was significantly reduced even in the presence of growth factors. Therefore, gonadotropin-supplemented MM medium was established as the base medium for the defined maturation condition. In Experiment 2, the addition of growth factors to gonadotropin-supplemented MM medium supported similar maturation (~90%) compared to the undefined condition (FBS-carrying). In Experiment 3, the addition of growth factors to embryo culture medium showed similar in vitro competence compared to the undefined (FBS) control. In Experiment 4, completely defined conditions (absence of FBS and BSA during in vitro maturation and embryo culture) were tested. A higher cleavage was observed with FGF2 (86%) compared to EGF (77%) and the FBS control (77%), but similar blastocyst rates were observed for FGF2 (24%), EGF (19%) and the FBS control (25%). Embryo quality was similar among groups. Finally, post-thawing survival was higher for FGF2 (94%) compared to the FBS control (77%). Thus, we report a simple defined IVP system for bovine species that generates developmental outcomes and embryos of similar quality than those produced under conditions containing FBS.

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Section: Discussionmentioning

confidence: 99%

Simple and Efficient Chemically Defined In Vitro Maturation and Embryo Culture System for Bovine Embryos

Arias

Vargas

Gallardo

et al. 2022

Animals

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“…Importantly, FGF17 belongs to the FGF8 subfamily and likely activates the same receptor as FGF8 (Zhang et al, 2006). Moreover, follicle stimulating hormone and oocytes stimulate the expression of FGF2 in bovine cumulus cells, whereas supplementation with recombinant FGF2 promotes the meiotic resumption of oocytes and suppresses PTGS2 and TNFAIP6 expression during maturation cultures (Barros et al, 2019). This suggests that FGF2 exerts suppressive effects on cumulus expansion in cattle.…”

Section: Discussionmentioning

confidence: 99%

Effect of fibroblast growth factor signaling on cumulus expansion in mice in vitro

Kanke

Fujii

Naito

et al. 2022

Molecular Reproduction Devel

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The expansion of cumulus cells associated with oocytes is an essential phenomenon in normal mammalian ovulation. Indeed, attenuated expression of cumulus expansion‐related genes, including Has2, Ptgs2, Ptx3, and Tnfaip6, results in ovulation failure, leading to female subfertility or infertility. Moreover, emerging evidence suggests that proteins of the fibroblast growth factor (FGF) family, produced within ovarian follicles, regulate the development and function of cumulus cells; however, the effects of FGF signaling on cumulus expansion have not been investigated extensively. Herein, we investigate the effects of FGF signaling, particularly those of FGF8 secreted by oocytes, on epidermal growth factor‐induced cumulus expansion in mice. The phosphorylation level of MAPK3/1, an intracellular mediator of FGF signaling, was significantly decreased in cumulus‐oocyte complexes (COCs) following treatment with NVP‐BGJ398, an FGF receptor inhibitor. Moreover, even though NVP‐BGJ398 treatment did not affect cumulus cell expansion, it significantly upregulated the expression of Ptgs2 and Ptx3. In contrast, treatment with recombinant FGF8 did not affect the degree of cumulus expansion or the expression of expansion‐related genes in COCs or oocytectomized cumulus cell complexes. Collectively, these results suggest that FGFs, other than FGF8, exert suppressive effects on the cumulus expansion process in mice.

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“…These data suggest that the expansion of TE in early development depends on FGF in mice, and that this expansion, which is dependent on FGF, occurs more in late stages in humans. The actions of FGF family members begin in the initial steps of reproduction mechanisms, therefore, FGF-10 expression has been detected in theca cells and ovarian stromal cells in humans [ 88 ], and FGF-2 and FGF-10 have been shown to increase the survival and proliferation of cumulus cells, increasing blastocyst development rates in bovine, sheep, and yak, in in vitro cultures [ 89 , 90 , 91 , 92 ]. Another family member, FGF-18, seems to regulate steroidogenesis in the fetal ovary [ 93 ].…”

Section: Growth Factors and Early Developmentmentioning

confidence: 99%

Pluripotency and Growth Factors in Early Embryonic Development of Mammals: A Comparative Approach

Llobat

2021

Veterinary Sciences

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The regulation of early events in mammalian embryonic development is a complex process. In the early stages, pluripotency, cellular differentiation, and growth should occur at specific times and these events are regulated by different genes that are expressed at specific times and locations. The genes related to pluripotency and cellular differentiation, and growth factors that determine successful embryonic development are different (or differentially expressed) among mammalian species. Some genes are fundamental for controlling pluripotency in some species but less fundamental in others, for example, Oct4 is particularly relevant in bovine early embryonic development, whereas Oct4 inhibition does not affect ovine early embryonic development. In addition, some mechanisms that regulate cellular differentiation do not seem to be clear or evolutionarily conserved. After cellular differentiation, growth factors are relevant in early development, and their effects also differ among species, for example, insulin-like growth factor improves the blastocyst development rate in some species but does not have the same effect in mice. Some growth factors influence genes related to pluripotency, and therefore, their role in early embryo development is not limited to cell growth but could also involve the earliest stages of development. In this review, we summarize the differences among mammalian species regarding the regulation of pluripotency, cellular differentiation, and growth factors in the early stages of embryonic development.

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Fibroblast growth factor 2 regulates cumulus differentiation under the control of the oocyte

Cited by 21 publications

References 50 publications

Simple and Efficient Chemically Defined In Vitro Maturation and Embryo Culture System for Bovine Embryos

Simple and Efficient Chemically Defined In Vitro Maturation and Embryo Culture System for Bovine Embryos

Effect of fibroblast growth factor signaling on cumulus expansion in mice in vitro

Pluripotency and Growth Factors in Early Embryonic Development of Mammals: A Comparative Approach

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