Fibroblast growth factor-2 increases the expression of neurogenic genes and promotes the migration and differentiation of neurons derived from transplanted neural stem/progenitor cells

Vergaño-Vera, Eva; Méndez-Gómez, Héctor; Hurtado-Chong, Anahí; Cigudosa, Juan C.; Vicario‐Abejón, Carlos

doi:10.1016/j.neuroscience.2009.03.033

Cited by 41 publications

(87 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Indeed, the number of BrdU + cells in the SVZ of + /Sey Dey mice was significantly reduced by 30% ( Fig. 1H; [9]. The neurospheres formed in aOBSC cultures prepared from P75 to P90 + /Sey Dey and + / + mice were analyzed after a 1 h BrdU pulse, and the PI and BrdU + cells were quantified in confocal planes (Fig.…”

Section: Resultsmentioning

confidence: 98%

“…Adult olfactory bulb stem cells (aOBSCs) were prepared from OBs dissected out of P75 + /Sey Dey and + / + littermate mice as previously described [5,9]. The OBs were digested with papain and disaggregated, and the cell suspension was plated in uncoated six-well plates in Dulbecco's modified Eagle's medium (DMEM) with F12 and N2 supplements (Invitrogen), plus E13.5 OBSC conditioned medium (1:1), and in the presence of fibroblast growth factor-2 (FGF-2, 20 ng/mL; PeproTech) and epidermal growth factor (EGF, 20 ng/mL; PeproTech).…”

Section: Nsc Culturementioning

confidence: 99%

“…To determine the capacity of aOBSCs to differentiate into neurons, astrocytes, and oligodendrocytes, cells from control and mutant mice were disaggregated and seeded at the same density in the absence of mitogens before they were fixed with 4% PFA after 1, 2, and 4 DIV. To analyze the neuronal subtypes, aOBSCs were plated and cultured for 7 days in a medium composed of DMEM/F12/ N2/B27 with 2% fetal bovine serum (FBS) and a single dose of FGF-2 (20 ng/mL) [9]. Clonal analysis of aOBSC neurospheres was performed as previously described [52].…”

Section: Nsc Culturementioning

confidence: 99%

“…Once in the OB, these neuroblasts differentiate into several neurochemical interneuron subtypes of granule and juxtaglomerular neurons [1][2][3]. Additional sources of interneurons may include the elbow of the rostral migratory stream (RMS) and the OB itself [4][5][6][7][8][9][10][11]. Adult neurogenesis is tightly regulated by both cell extrinsic and intrinsic mechanisms, among which transcription factors (TFs) play a major role, participating in several aspects of NSC maintenance, fate choice, and neuronal differentiation [12].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Pax6 Is Essential for the Maintenance and Multi-Lineage Differentiation of Neural Stem Cells, and for Neuronal Incorporation into the Adult Olfactory Bulb

Curto

Nieto‐Estévez

Hurtado-Chong

et al. 2014

Stem Cells and Development

View full text Add to dashboard Cite

The paired type homeobox 6 (Pax6) transcription factor (TF) regulates multiple aspects of neural stem cell (NSC) and neuron development in the embryonic central nervous system. However, less is known about the role of Pax6 in the maintenance and differentiation of adult NSCs and in adult neurogenesis. Using the + /Sey Dey mouse, we have analyzed how Pax6 heterozygosis influences the self-renewal and proliferation of adult olfactory bulb stem cells (aOBSCs). In addition, we assessed its influence on neural differentiation, neuronal incorporation, and cell death in the adult OB, both in vivo and in vitro. Our results indicate that the Pax6 mutation alters Nestin + -cell proliferation in vivo, as well as self-renewal, proliferation, and survival of aOBSCs in vitro although a subpopulation of + /Sey Dey progenitors is able to expand partially similar to wild-type progenitors. This mutation also impairs aOBSC differentiation into neurons and oligodendrocytes, whereas it increases cell death while preserving astrocyte survival and differentiation. Furthermore, Pax6 heterozygosis causes a reduction in the variety of neurochemical interneuron subtypes generated from aOBSCs in vitro and in the incorporation of newly generated neurons into the OB in vivo. Our findings support an important role of Pax6 in the maintenance of aOBSCs by regulating cell death, self-renewal, and cell fate, as well as in neuronal incorporation into the adult OB. They also suggest that deregulation of the cell cycle machinery and TF expression in aOBSCs which are deficient in Pax6 may be at the origin of the phenotypes observed in this adult NSC population.

show abstract

Section: Resultsmentioning

confidence: 98%

Section: Nsc Culturementioning

confidence: 99%

Section: Nsc Culturementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Pax6 Is Essential for the Maintenance and Multi-Lineage Differentiation of Neural Stem Cells, and for Neuronal Incorporation into the Adult Olfactory Bulb

Curto

Nieto‐Estévez

Hurtado-Chong

et al. 2014

Stem Cells and Development

View full text Add to dashboard Cite

show abstract

“…Several signaling molecules have been linked with this activity, and several of the aforementioned factors utilize MAP kinases (MAPKs), phosphoinositide 3-kinase (PI3K), Rho-kinase, or a combination of these pathways to control migration rates (Hayashi et al 2007. The potential involvement of FGFs in trophoblast cell migration has not been examined in ruminant species, but specific FGFs regulate cell migration in other systems (Bottcher & Niehrs 2005, Tao et al 2005, Natanson-Yaron et al 2007, Kuriyama & Mayor 2009, Vergano-Vera et al 2009). Also, an embryonic lethal phenotype exists in mice lacking FGF receptor 1 because of failures in extraembryonic and mesoderm cell migration during gastrulation .…”

Section: Introductionmentioning

confidence: 99%

Fibroblast growth factors activate mitogen-activated protein kinase pathways to promote migration in ovine trophoblast cells

Yang

Giassetti²,

Ealy

2011

Reproduction

View full text Add to dashboard Cite

Fibroblast growth factors (FGFs) 2 and FGF10 are uterine-and conceptus-derived factors that mediate trophoblast activities in cattle and sheep. To extend our understanding of how FGFs may control peri-implantation development in ruminants, we determined whether FGF2 and FGF10 impact trophoblast cell migration. Transwell inserts containing 8 mm pores were used to examine whether FGF2 or FGF10 supplementation increased oTr1 cell migration. Supplementation with 0.5 ng/ml FGF2 or FGF10 did not affect oTr1 cell migration number, but exposure to 5 or 50 ng/ml FGF2 or FGF10 increased (P!0.05) oTr1 cell migration when compared with controls. The involvement of specific MAP kinase (MAPK) cascades in mediating this FGF response was examined by using pharmacological inhibitors of specific MAPKs. Western blot analysis indicated that FGF2 and FGF10 increased phosphorylation status of MAPKs 1, 3, 8, 9, and 14. Exposure to specific inhibitors blocked FGF induction of each MAPK. Exposure to inhibitors before supplementation with FGF2 or FGF10 prevented FGF induction of cell migration, indicating that each of these signaling molecules was required for FGF effects. A final series of studies examined whether FGF2 and FGF10 also mediated the migration of a bovine trophoblast line (CT1 cell). Increases in migration were detected in each cell line by supplementing 5 or 50 ng/ml FGF2 or FGF10 (P!0.05). In summary, FGF2 and FGF10 regulate migratory activity of ovine trophoblast cells through MAPK-dependent pathways. These outcomes provide further evidence that FGFs function as mediators of peri-implantation conceptus development in cattle and sheep.

show abstract

Transcriptional Regulation of Olfactory Bulb Neurogenesis

Díaz-Guerra

Pignatelli

Nieto‐Estévez

et al. 2013

The Anatomical Record

View full text Add to dashboard Cite

The neurons in the olfactory bulb originate from molecularly defined and spatially distinct proliferative regions. Glutamatergic projection neurons are generated during the embryonic period in the local ventricular zone of the olfactory bulb, a territory in the dorsal telencephalon in which the transcription factor Pax6 is expressed. Some cells in this zone also express Tbr1, a marker of glutamatergic neurons. By contrast, embryonic olfactory bulb interneurons are derived from Gsx2 expressing cells in the dorsal lateral ganglionic eminence of the ventral telencephalon, and from progenitors outside the dorsal lateral ganglionic eminence, including the olfactory bulb neuroepithelium. Postnatally, interneurons arise from the subventricular zone of the lateral ventricle, although the rostral migratory stream and the olfactory bulb also appear to serve as a source of neurons. Transcription factors are crucial to generate all classes of neurons and glia in the olfactory bulb, both during development and adulthood. In this article, we discuss and propose models on how the spatial and temporal regulation of transcription factor expression controls the self-renewal, proliferation and cell fate of neural stem cells and progenitors, which finally leads to the generation of distinct functional subtypes of neurons in the developing and adult olfactory bulb. Anat Rec, 296:1364Rec, 296: -1382Rec, 296: , 2013. V C 2013 Wiley Periodicals, Inc.Key words: olfactory bulb; neurogenesis; transcription factor; neural stem cells; proliferation; differentiation

show abstract

Fibroblast growth factor-2 increases the expression of neurogenic genes and promotes the migration and differentiation of neurons derived from transplanted neural stem/progenitor cells

Cited by 41 publications

References 60 publications

Pax6 Is Essential for the Maintenance and Multi-Lineage Differentiation of Neural Stem Cells, and for Neuronal Incorporation into the Adult Olfactory Bulb

Pax6 Is Essential for the Maintenance and Multi-Lineage Differentiation of Neural Stem Cells, and for Neuronal Incorporation into the Adult Olfactory Bulb

Fibroblast growth factors activate mitogen-activated protein kinase pathways to promote migration in ovine trophoblast cells

Transcriptional Regulation of Olfactory Bulb Neurogenesis

Contact Info

Product

Resources

About