Fibroblast Growth Factor-2 (FGF-2) Expression in Pterygia Using Cell Spot Arrays

Abstract

Fibroblast growth factor (FGF) is a main regulator of cell differentiation, cell migration and angiogenesis in normal and abnormal conjunctiva epithelia, but specific mechanisms of its aberrant expression are yet to be investigated. In the present study, we investigated FGF-2 protein expression within several pterygia. Using a liquid-based cytology assay, we obtained cell specimens from pterygia and healthy tissues directly from patients. A combination of immunocytochemistry followed by digital image analysis showed significant overexpression of FGF-2 in all the examined pterygia. In 30/60 (50%) cases there were high levels of staining intensity, whereas in the remaining 30/60 (50%) cases there were moderate levels of expression. FGF-2 levels of the control group were significantly lower in comparison with the pterygia group. There was no significant correlation between FGF-2 levels and either sex or location of the pterygium. FGF-2 levels had a significant correlation with morphological characteristics of the pterygia. More specifically, FGF-2 levels were significantly higher in the pterygia with a fleshy morphology. Interestingly, recurrent lesions demonstrated high expression levels. An overexpression of FGF-2 has been observed frequently in pterygia, where it may play a crucial role in determining the lesion’s progression. FGF-2 upregulation correlates with the morphology of pterygia and its tendency to recur. Cell spot analysis based on liquid-based cytology is a simple, yet effective, method for detecting a broad spectrum of protein markers and could be useful in analyzing potential pterygia patient samples.

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“…The balance of pro-and anti-angiogenic factors determines the ability of these cancer cells. Vascular Endothelial Growth Factor (VEGF) [64], Platelet-derived Growth Factor (PDGF) [65], acidic and basic fibroblast growth factors (FGF 1 and 2) [66], and IL-8 are pro-angiogenic factors [67]. Interferons, proteolytic fragments such as angiostatin and endostatin, and thrombospondin-1 are all negative regulators of angiogenesis [1,64,65,66,68].…”

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“…The balance of pro-and anti-angiogenic factors determines the ability of these cancer cells. Vascular Endothelial Growth Factor (VEGF) [64], Platelet-derived Growth Factor (PDGF) [65], acidic and basic fibroblast growth factors (FGF 1 and 2) [66], and IL-8 are pro-angiogenic factors [67]. Interferons, proteolytic fragments such as angiostatin and endostatin, and thrombospondin-1 are all negative regulators of angiogenesis [1,64,65,66,68].…”