Fibroblast growth factor-1 improves the survival and regeneration of rat vagal preganglionic neurones following axon injury

Jacques, Thomas S.; Skepper, Jeremy N.; Navaratnam, V.

doi:10.1016/s0304-3940(99)00832-0

Cited by 19 publications

(17 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, after perinatal cervical vagotomies, implants of embryonic GI tract tissue into the neck near the stump of the transected vagus are able to increase preganglionic survival (Rinaman and Levitt, 1991). Furthermore, both levocartinine (Fernandez et al, 1992) and fibroblast growth factor (Jacques et al, 1999) treatments rescue some DMNX neurons. Whether these latter effects are limited to the cell bodies or whether the treatments can potentiate regeneration into the target tissues is unclear, although fibroblast growth factor has been shown to increase at least the survival of axons in the vagal trunk.…”

Section: Vagal Efferents In Contrast To Afferents Fail To Regeneratmentioning

confidence: 99%

“…Experiments designed to maximize the recovery of vagal efferent function after axotomy may need to use nerve suture (e.g., Nanobashvili et al, 1994 ), exogenous neurotrophin therapy (e.g., Jacques et al, 1999), or another technique to promote efferent regeneration. In contrast, experiments designed to minimize efferent recovery need to divide the nerve with a procedure that leaves a gap between the proximal and distal stumps.…”

Section: Implications For Structure-function Analyses Using Vagotomymentioning

confidence: 99%

See 1 more Smart Citation

Long‐term regeneration of abdominal vagus: Efferents fail while afferents succeed

Phillips

Baronowsky

Powley

2002

J of Comparative Neurology

View full text Add to dashboard Cite

Vagal afferents regenerate, by 18 weeks after subdiaphragmatic transection, to reinnervate the gut and to differentiate into the two types of terminals normally found in the smooth muscle wall of the gastrointestinal (GI) tract (Phillips et al. [2000] J Comp Neurol. 421:325-346). Regeneration, however, is neither complete nor entirely accurate by 18 weeks. Moreover, the capacity of the vagal efferents to reinnervate the GI tract under comparable conditions has not been evaluated. Therefore, to determine whether a more extended postaxotomy survival interval would (1). result in more extensive reinnervation of smooth muscle, (2). facilitate correction of the inaccuracies of the regenerated axons and terminals, and (3). yield motor as well as sensory reinnervation of GI targets, Sprague-Dawley rats received either complete subdiaphragmatic vagotomies (n = 18) or sham surgeries (n = 12). Physiological endpoints that might normalize as vagal elements regenerated, including body weight, daily food intake, size of first daily meal, and metabolic efficiency, were monitored. At 45 weeks after the vagotomies, the animals were randomly assigned to afferent (wheat germ agglutinin-horseradish peroxidase) or efferent (cholera toxin subunit B-horseradish peroxidase) mapping conditions, and labeled axons and terminals in the stomach and first 8 cm of the small intestine were inventoried in whole-mounts. Afferent regeneration was more extensive at 45 weeks than previously observed at 18 weeks after surgery; however, the amount of GI innervation was still not comparable to the intact pattern of the sham rats. Furthermore, abnormal patterns of sensory organization occurred throughout the reinnervated field, with small bundles of axons forming complex tangles and some individual axons terminating in ectopic locations. The presence of growth cone profiles suggested that vagal reorganization was ongoing even 45 weeks after surgery. In contrast to this relatively extensive, albeit incomplete, sensory reinnervation of the gut, motor fibers had failed to reinnervate the GI tract. Thus, dramatic differences exist in the regenerative capacities of the sensory and motor arms of the vagus under the same surgical and maintenance conditions. Furthermore, the functional measures of disordered energy regulation did not normalize over the 45 weeks during which afferent but not efferent innervation was restored.

show abstract

Section: Vagal Efferents In Contrast To Afferents Fail To Regeneratmentioning

confidence: 99%

Section: Implications For Structure-function Analyses Using Vagotomymentioning

confidence: 99%

Long‐term regeneration of abdominal vagus: Efferents fail while afferents succeed

Phillips

Baronowsky

Powley

2002

J of Comparative Neurology

View full text Add to dashboard Cite

show abstract

“…It takes part in multiple biological processes, including embryonic development, cell growth, morphogenesis, angiogenesis and tissue repair. Acidic FGF has been examined as therapeutic protein in animal and clinical trials for skin wound and burn healing (44), wound healing of diabetic patients (62), angiogenesis (22,49), neurotrophic activity (30,64), strong anti-apoptotic function (14,68), gut epithelium protection (20), spinal cord injury repair (65). New lymph vessels growth as well as recovery of injured ischemic blood vessels is increased when using therapy with aFGF (29).…”

Section: Introductionmentioning

confidence: 99%

Expression of The Human Acidic Fibroblast Growth Factor in Transgenic Tomato and Safety Assessment of Transgenic Lines

Stoykova

Radkova

Stoeva-Popova

et al. 2011

Biotechnology & Biotechnological Equipment

View full text Add to dashboard Cite

“…FGF1, a member of the FGF family, is a strong mitogen for glial cells and exerts potent trophic effects on neurons [4, 10, 12, 18]. Jacques et al demonstrated that exogenous FGF1 increased the number of axons regenerating the injured vagal nerve and the number of neurons surviving in the DMNV at nine weeks after injury [10].…”

Section: Introductionmentioning

confidence: 99%

“…Jacques et al demonstrated that exogenous FGF1 increased the number of axons regenerating the injured vagal nerve and the number of neurons surviving in the DMNV at nine weeks after injury [10]. Since FGF1 lacks the signal peptide, FGF1 is thought to be released upon cellular injury and to have a trophic effect on damaged neurons [3, 9].…”

Section: Introductionmentioning

confidence: 99%

Comparison of FGF1 (aFGF) Expression between the Dorsal Motor Nucleus of Vagus and the Hypoglossal Nucleus of Rat

Toyoda

Okano

Bamba

et al. 2006

Acta Histochem. Cytochem

View full text Add to dashboard Cite

Neurons in the dorsal motor nucleus of the vagus (DMNV) are more severely affected by axonal injury than most other nerves, such as those of the hypoglossal nucleus. However, the mechanism underlying such a response remains unclear. In this study, we compared the expression of fibroblast growth factor 1 (FGF1), a neurotrophic factor, between the DMNV and the hypoglossal nucleus by RT-PCR and immunohistochemical analyses. RT-PCR showed that the level of FGF1 mRNA expression in the DMNV was lower than that in the hypoglossal nucleus (P0.01). Immunohistochemistry revealed that FGF1 was localized to neurons. FGF1-positive neurons in large numbers were evenly distributed in the hypoglossal nucleus, whereas FGF1-positive neurons were located in the lateral part of the DMNV. Double immunostaining for FGF1 and choline acetyltransferase demonstrated that 22.7% and 78% of cholinergic neurons were positive for FGF1 in the DMNV and hypoglossal nucleus, respectively. A tracing study with cholera toxin B subunit (CTb) demonstrated that cholinergic neurons sending their axons from the DMNV to the superior laryngeal nerve were FGF1-negative. The results suggest that the low expression of FGF1 in the DMNV is due to severe damage of neurons in the DMNV.

show abstract

Fibroblast growth factor-1 improves the survival and regeneration of rat vagal preganglionic neurones following axon injury

Cited by 19 publications

References 14 publications

Long‐term regeneration of abdominal vagus: Efferents fail while afferents succeed

Long‐term regeneration of abdominal vagus: Efferents fail while afferents succeed

Expression of The Human Acidic Fibroblast Growth Factor in Transgenic Tomato and Safety Assessment of Transgenic Lines

Comparison of FGF1 (aFGF) Expression between the Dorsal Motor Nucleus of Vagus and the Hypoglossal Nucleus of Rat

Contact Info

Product

Resources

About