Fibroblast cell attachment and growth on nanoengineered sculptured thin films

Demirel, Melik C.; So, Eric; Ritty, Timothy M.; Naidu, Sanjiv H.; Lakhtakia, Akhlesh

doi:10.1002/jbm.b.30656

Cited by 50 publications

(47 citation statements)

References 22 publications

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“…Depending on the application of an implant, various surface modification techniques of Parylene have been proposed to improve the biocompatibility of an encapsulated implant. (8,(11)(12)(13) The surface roughness is also an important factor that affects the biocompatibility of a Parylene-encapsulated implant and was thus investigated as a function of dimer sublimation rate in this work.…”

Section: Introductionmentioning

confidence: 99%

Effect of Thermal and Deposition Processes on Surface Morphology, Crystallinity, and Adhesion of Parylene-C

Hsu

Rieth

Kammer

et al. 2008

Sensors and Materials

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Neural interface devices have been developed for neural science and neuroprosthetics applications to record and stimulate neural signals. Chemical-vapor-deposited Parylene-C films were studied as an encapsulation material for such an implantable device. The surface morphology of an implant affects its biocompatibility; thus, the Parylene-C surface morphology was investigated as a function of the precursor sublimation rate by atomic force microscopy. We found that high precursor sublimation rates resulted in slightly higher root-mean-square surface roughnesses (from 5.78 to 9.53 nm for deposition rates from 0.015 to 0.08 g/min). The crystallinity affects the physical properties of semicrystalline polymers, and various heat treatments were found to modify the crystallinity of Parylene-C films, as assessed by X-ray diffraction (XRD). The XRD peak at 2θ = ~14.5° increased in intensity and decreased in full width at half maximum with increasing annealing temperature, indicating an increase in film crystallinity. Poor adhesion would compromise the protection offered by Parylene-C coatings. The adhesion between Parylene-C and silicon, amorphous silicon carbide, and boron silicate glass substrates were evaluated using the standard tape adhesion test from the American Society for Testing and Materials (ASTM) in an attempt to minimize the occurrence of delamination failures. The tape adhesion tests indicated strong adhesion for all the as-deposited Parylene films with the application of an adhesion promoter (Silquest A-174 ® silane). However, annealing the deposited films at temperatures from 85 to 150°C in air for 20 min reduced film adhesion, and also the adhesion testing procedure used significantly affects the results obtained. Supporting evidence suggested that the thermal stress generated in the films weakened the adhesive force. We concluded that 88 Sensors and Materials, Vol. 20, No. 2 (2008) the Parylene-C film properties (surface morphology, crystallinity, and adhesion) changed during deposition and thermal annealing, suggesting that the Parylene-C film properties can be tailored and that, with care, failure due to film delamination can be avoided.

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Section: Introductionmentioning

confidence: 99%

Effect of Thermal and Deposition Processes on Surface Morphology, Crystallinity, and Adhesion of Parylene-C

Hsu

Rieth

Kammer

et al. 2008

Sensors and Materials

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“…In general, polymer chains comprising amorphous film surface regions can provide accessible pyridine adsorption sites through formation of noncovalent ligand-polymer interactions. [17,18] Enhanced surface disorder expected for higher surface energy (i.e., high curvature, approximately ∼150 nm diameter) NTF polymer filaments [14,15] provides a potential mechanism for more rapid adsorption of larger quantities of ligand relative to a planar PPX-C film (Fig. 2, Figure 2.…”

mentioning

confidence: 99%

“…1). [14,15] Figure 2 is a schematic representation of our approach. In general, polymer chains comprising amorphous film surface regions can provide accessible pyridine adsorption sites through formation of noncovalent ligand-polymer interactions.…”

mentioning

confidence: 99%

“…Although Ni morphology, topology, and surface chemistry are critically important for these applications, their control is limited by: 1) Ni deposition conditions and treatments, [7][8][9] and 2) properties and available architectures of sacrificial metallization templates, [1,6,[10][11][12][13] which are usually removed by (thermo)chemical treatments after plating. In this regard, we have been exploring poly(p-xylylene) nanostructured thin films (NTFs) formed through vaporphase polymerization and directed deposition of [2.2]paracyclophane derivatives [14,15] (Fig. 1A) as new metallization templates.…”

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confidence: 99%

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Noncovalent Deposition of Nanoporous Ni Membranes on Spatially Organized Poly(p‐xylylene) Film Templates

et al. 2007

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Nickel metal is widely used in catalytic, [1,2] energy storage, [3,4] and optical applications [5,6] due to its favorable physicochemical properties. Although Ni morphology, topology, and surface chemistry are critically important for these applications, their control is limited by: 1) Ni deposition conditions and treatments, [7][8][9] and 2) properties and available architectures of sacrificial metallization templates, [1,6,[10][11][12][13] (Fig. 1B-C). Here, we present our initial results relating to the fabrication and characterization of nanoporous Ni membranes templated by conformal electroless (EL) metallization of poly(chloro-p-xylylene) (PPX-C) NTFs. EL metallization of polymer films is typically a multistep process [16] involving: 1) chemical or mechanical surface microroughening to promote metal adhesion; 2) adsorption of Pd/Sn core/shell colloids to the surface; 3) selective dissolution of the Sn II/IV b-hydroxy shell segment not anchoring the colloid to the surface to expose the catalytic Pd 0 core; and finally 4) solution deposition of EL metal. For NTFs, however, the need to minimize potential damage to film nanoarchitectures ( Fig. 1), eliminate environmentally hazardous Sn salts, and reduce process steps and costs necessitates consideration of an alternate EL plating procedure. In one such process, [17][18][19][20][21] solvent-templated sites tailored to adsorb catalyst-binding pyridine ligands are first created at a polymer surface during film formation. Partitioning of pyridine from aqueous solution into these sites, driven by maximization of hydrophobic van der Waals and p-p interactions with the polymer aromatic functional groups that define the sites, noncovalently binds pyridine ligands at the polymer surface. Because the hydrophilic N site of the adsorbed pyridine remains accessible to aqueous solution, covalent binding of Pd II EL COMMUNICATION

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“…Изучение роста клеток in vitro на полимерных плёнках. Для оценки биосовместимости пленок были использованы фибробласты зелёной мартышки линии COS-1 ("Биолот", Россия) [28,29]. Клетки культивировались в среде DMEM (Dubecco's Modified Eagle Medium, "Invitrogen") с повышенным содержанием глюкозы (4,5 г/л), содержащей 10% эмбриональной телячьей сыворотки (ЭТС), 100 МЕ/мл пенициллина и 100 мкг/мл стрептомицина ("Invitrogen").…”

Section: методикаunclassified

The effect of poly(3-hydroxybutyrate) modification by poly(ethylene glycol) on the viability of cells grown on the polymer films

Zharkova

et al. 2012

BIOMED KHIM

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Биоразлагаемый полимер бактериального происхождения, поли-3-оксибутират (ПОБ), активно исследуется как биоматериал для тканевой инженерии. Однако факторы, определяющие биосовместимость этого полимерного материала, в настоящее время остаются не до конца ясны. С целью исследования влияния свойств поли-3-оксибутирата на жизнеспособность культивируемых на нем клеток была проведена модификация полимерного материала с использованием гидрофильного полимера полиэтиленгликоля 300 (ПЭГ). Были получены композиты ПОБ/ПЭГ с различным содержанием ПЭГ (10, 20, 30 и 50%), после чего 95% ПЭГ было удалено из композитов в ходе инкубации в воде. Были исследованы шероховатость и гидрофильность поверхности пленок методами атомно-силовой микроскопии и измерения контактного угла смачивания, соответственно. Была исследована биосовместимость плёнок in vitro на культуре фибробластов линии COS-1. Показано, что как шероховатость полимерной поверхности, так и ее гидрофобность прямо пропорциональны исходному содержанию ПЭГ в композитах ПОБ/ПЭГ. Показано, что интенсивность роста фибробластов линии COS-1 на полимерных пленках определяет сочетание двух основных физико-химических свойств полимерной поверхности: шероховатости и гидрофильности. Оптимальной шероховатостью для роста клеток линии COS-1 является средняя шероховатость более 25 нм, предельных значений контактного угла смачивания, ответственных за относительно высокую жизнеспособность клеток, найдено не было. Это свидетельствует о том, что наибольшее влияние на рост клеток оказывает шероховатость поверхности пленок, а уровень гидрофильности полимерной поверхности вызывает дополнительный положительный эффект на жизнеспособность прикрепившихся на плёнках клеток через посредство формирования на ней слоя адсорбированного белка. Таким образом, модификация полимерного материала ПОБ с использованием ПЭГ привело к улучшению жизнеспособности клеток, культивируемых на полимерных пленках in vitro. Полученные данные можно использовать для разработки таких медицинских изделий, как хирургические заплаты и пародонтологические мембраны.Ключевые слова: поли-3-оксибутират (ПОБ), полиэтиленгликоль (ПЭГ), шероховатость поверхности, гидрофильность поверхности, биосовместимость, COS-1. 579* -адресат для переписки

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Fibroblast cell attachment and growth on nanoengineered sculptured thin films

Cited by 50 publications

References 22 publications

Effect of Thermal and Deposition Processes on Surface Morphology, Crystallinity, and Adhesion of Parylene-C

Effect of Thermal and Deposition Processes on Surface Morphology, Crystallinity, and Adhesion of Parylene-C

Noncovalent Deposition of Nanoporous Ni Membranes on Spatially Organized Poly(p‐xylylene) Film Templates

The effect of poly(3-hydroxybutyrate) modification by poly(ethylene glycol) on the viability of cells grown on the polymer films

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