Fibroblast and osteoblast adhesion and morphology on calcium phosphate surfaces

Abstract

Implant loosening in bone fixation is an unresolved complication associated with internal fixation. It is generally accepted that this problem can be overcome by modifying the implant/bone interface for improved osseointegration. This is achieved, in part, by hydroxyapatite (HA) or tricalcium phosphate coatings. Unfortunately, the benefits of these coatings are constrained by not only the generally low strength of their adhesion to the implant surface but also the limited cohesion within their layers. Anodic Plasmachemical treatment (APC) has been developed to incorporate electrolytes and produce coatings with various microtopographies and strong adhesion to implants. In this in vitro study fibroblast and osteoblast morphologies and adhesion to various substrates were evaluated using qualitative and quantitative methods. The substrates were Thermanox plastic and commercially pure titanium. The latter were surface-treated using several different methods: conventional anodisation, plasma spraying of HA and anodic plasma-chemical (APC) treatment in an electrolyte solution containing either calcium and phosphate (APCCaP) or phosphoric acid (APC-P). Both osteoblasts and fibroblasts showed extensive cell spreading, total cell area and greatest amount of adhesion, with defined adhesion patterns on the Thermanox plastic, anodised titanium, and the two APC-CaP substrates. With fibroblasts, almost no cell spreading and very low adhesion, was observed in cells cultured on the APC-P and HA surfaces. The extent of cell spreading correlated with the area of focal adhesions as assessed by the amount of vinculin labelling. The Thermanox plastic, anodised titanium, and the two APCCaP substrates were the most cytocompatible substrates with regard to this in vitro evaluation.

Evaluation of Multifunctional Polysaccharide Hydrogels with Varying Stiffness for Bone Tissue Engineering

“…3C, D) increased with increasing stiffness gels by 7.8 and 6.4 times, respectively. It has been shown that osteoblasts and fibroblasts prefer stiffer surfaces for proliferation [38][39][40] and according to the proliferation data obtained via the cell titer blue kit, we saw that the 0.5% (w/v) genipin crosslinked hydrogel group showed significantly higher rates of cellular proliferation ( p < 0.05, n = 3) over 24 h for osteoblasts and fibroblasts.…”

Amorphous Ca2+ polyphosphate nanoparticles regulate the ATP level in bone-like SaOS-2 cells

“…3A,B,D,E). The thin sections revealed that the lobulated osteoblast-like SaOS-2 cells (Baxter et al, 2002) from untreated cells contained only a low number of mitochondria (Fig. 3A,B), whereas those cells that had been exposed to aCa-polyP-NP contained dense clusters of mitochondria ( Fig.…”

Quantitative Analysis of Growth of Cells on Physicochemically Modified Surfaces

“…All of the adherent cells over the modified substrate respond to the cell viability, growth rate, and proliferation. [18][19][20] In this study, we describe the correlation between the cell's response and the surface properties. We used different model surfaces with different degrees of wettability and microstructures to examine their influence on the adhesion and proliferation of cells.…”