Fibrinolytic enzyme(s) in western diamondback rattlesnake (Crotalus atrox) venom

Bajwa, S.S.; Markland, Francis S.; Russell, Findlay E.

doi:10.1016/0041-0101(80)90007-0

Cited by 75 publications

(22 citation statements)

References 11 publications

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“…Fibrinolytic activity assays were measured with the fibrin-plate clearance method (12). Positive and negative controls were plasmin and 0.9% NaCl respectively.…”

Section: Enzyme Activity Assaysmentioning

confidence: 99%

Acutolysin C, a weak hemorrhagic toxin from the venom of Agkistrodon acutus with leucoagglutination activity

Wei¹,

Chen²,

Jh³

2011

J. Venom. Anim. Toxins incl. Trop. Dis

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Abstract:The properties and agglutination activity of acutolysin C, a hemorrhagic metalloproteinase obtained from Agkistrodon acutus venom, were studied herein. Acutolysin C is a basic glycoprotein consisting of a single polypeptide chain with a molecular weight of 23.1 kDa and pI 8.7, containing one Zn 2+ and one Ca 2 + per molecule. It possesses caseinolytic, weak lethal (LD 50 = 7.6 mg/kg) and weak hemorrhagic (MHD = 12.0 μg) activities, but does not present fibrinolytic, fibrinogenolytic, arginine esterase and phospholipase A 2 actions. In addition, it revealed agglutination activity on some animal lymphocytes, including five species of mammals, six of birds, three of reptiles and one of amphibians, but had no effect on lymphocytes from two species of reptiles, one amphibian and nine species of fish. It had no effects on the erythrocytes and platelets of all 26 animal species tested. Both leucoagglutination and caseinolytic activities were inhibited by EDTA; while cysteine, 2-mercaptoethanol, 1,4-dithiothreitol, glutathione, serum against acutolysin C and serum against homologous snake venom as well as glucose, sucrose, mannose, lactose and galactose had no effects on inhibition. The lowest concentration of acutolysin C that induced mouse lymphocyte agglutination was 2.5 μg/mL. Acutolysin C is an interesting substance since it is the first member of the hemorrhagin family to be shown to have leucoagglutination activity.

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“…Fibrinolytic activity assays were measured with the fibrin-plate clearance method (12). Positive and negative controls were plasmin and 0.9% NaCl respectively.…”

Section: Enzyme Activity Assaysmentioning

confidence: 99%

Acutolysin C, a weak hemorrhagic toxin from the venom of Agkistrodon acutus with leucoagglutination activity

Wei¹,

Chen²,

Jh³

2011

J. Venom. Anim. Toxins incl. Trop. Dis

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“…Thrombin-like enzymes act as procoa gulants by converting fibrinogen to fibrin [1][2][3][4], These venoms also contain anticoagulant proteases with fibrinogenolytic or fibrino lytic activity [5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Biological and Thrombolytic Properties of Fibrolase -A New Fibrinolytic Protease from Snake Venom

Ahmed¹,

Gaddis²,

Tennant³

et al. 1990

Pathophysiol Haemos Thromb

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Fibrolase, a direct-acting fibrinolytic enzyme has been shown to cleave primarily the Aα and Bβ chains of human fibrin. We have previously reported that fibrolase also exhibits fibrinogenolytic activity and acts mainly as an α-chain fibrinogenase. In contrast to the action of streptokinase (plasminogen activator), fibrolase does not activate plasminogen. In vitro thrombolytic efficacy of fibrolase was determined by monitoring the release of radiolabel from iodinated fibrin and human blood clots. Fibrolase effectively digested the clots in a dose-dependent manner. The in vivo efficacy of fibrolase was evaluated in an animal model of arterial thrombosis. Fibrolase was found to be efficacious at dissolving femoral arterial clots following a single intravenous bolus administration. Time to reperfusion was dose dependent and similar to that observed with streptokinase. No adverse effects on blood pressure and heart rate were observed.

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“…1, Table 1). Minimal fibrinolytic dose (MFD) of the venom was tested by a method of Bajwa et al (1980) resulting with a MFD of 13 μg (Fig. 1A).…”

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confidence: 99%

Neutralization of lethality and proteolytic activities of Malayan pit viper (Calloselasma rhodostoma) venom with North American Virginia opossum (Didelphis virginiana) serum

Pornmanee

Sánchez

López

et al. 2008

Toxicon

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Malayan pit viper (Calloselasma rhodostoma) envenomation is a major health problem in South East Asia. During envenomation, venom components mainly affect the hemostatic system. The sera from the North American Virginia opossums (Didelphis virginiana) were able to neutralize the venom of the Malayan pit viper. These natural inhibitors could be explored as potential therapeutics against envenomations of a variety of venomous snake species in different geographical habitats.

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Fibrinolytic enzyme(s) in western diamondback rattlesnake (Crotalus atrox) venom

Cited by 75 publications

References 11 publications

Acutolysin C, a weak hemorrhagic toxin from the venom of Agkistrodon acutus with leucoagglutination activity

Acutolysin C, a weak hemorrhagic toxin from the venom of Agkistrodon acutus with leucoagglutination activity

Biological and Thrombolytic Properties of Fibrolase -A New Fibrinolytic Protease from Snake Venom

Neutralization of lethality and proteolytic activities of Malayan pit viper (Calloselasma rhodostoma) venom with North American Virginia opossum (Didelphis virginiana) serum

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