Fibrinogenolytic enzymes of Trimeresurus mucrosquamatus venom

Ouyang, Chaoho; Teng, Che-Ming

doi:10.1016/0005-2795(76)90321-4

Cited by 218 publications

(56 citation statements)

References 22 publications

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“…Extracts E1 and E2 were added separately to a solution of 0.1% human fibrinogen mixed with 50 mM of Tris-HCl buffer (at pH 7.5) (a volume of 1 mL) and a venom solution concentration of (20 µg) we used as reference the method of Ouyang and Teng (1976). The mixture was incubated in the presence at first and then in absence of extracts (1 mg) at 37°C.…”

Section: In Vitro Determination Of Essential Oil Effect On Fibrinogenmentioning

confidence: 99%

Anti-hemolytic and Anti-cytotoxic Effect of Two Artemisia Species (A. campestris and A. herba-alba) Essential Oil against Snake Venom

Jaouadi

Abdelkafi-Koubaa

Riabi-Ayari

et al. 2016

IJAB

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To cite this paper: Jaouadi, I., Z. Abdelkafi-Koubaa, S. Riabi-Ayari, I. Hassen, M.T. Yakoubi, M. El-Ayeb, M. El-Gazzah and N. Marrakchi, 2016. Antihemolytic and anti-cytotoxic effect of two Artemisia species (A. campestris and A. herba-alba) essential oil against snake venom. AbstractIn this study, we verify the possible activity of Artemisia herba-alba and A. campestris (Asteraceae) essential oil against the snake venoms. The leaves of A. campestris has been used in the folk medicine against snake moisture. The aerial part extracts of both plants have been tested for their action against snake venom. Interaction of A. campestris (E1) and A. herba alba (E2) essential oils with proteins extracted from snake venom has been tested. Lethality in mice, inflammation and cytotoxicity induced by Cerastes cerastes venoms were significantly inhibited by the mixture of different amounts of E1 and E2 and the venom. However, both extracts showed neutralization of the venoms hemolytic activity which is more important with E2. Chemical characterization of E1 and E2 revealed there is an important amount of polyphenols and tannins which are known by their bio-active effect. The result could lead as to think that these components have an action by inhibiting the toxicity of snake venoms in vitro. We can conclude therefore that the use of the essential oil from the two Artemisia species have a curative effect on the snake venom. These results confirmed the traditional use of A. campestris and we discovered a similar effect of A. herba-alba. No data have been reported till now about the essential oil curative effect of both species.

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Section: In Vitro Determination Of Essential Oil Effect On Fibrinogenmentioning

confidence: 99%

Anti-hemolytic and Anti-cytotoxic Effect of Two Artemisia Species (A. campestris and A. herba-alba) Essential Oil against Snake Venom

Jaouadi

Abdelkafi-Koubaa

Riabi-Ayari

et al. 2016

IJAB

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“…20 AqSEJ (0-20 μg) was incubated with the human plasma fibrinogen (50 μg) in a total volume 40 μL of 10 mM Tris-HCl buffer pH 7.4 for 4 hours at 37°C. After an incubation period, reaction was terminated by adding 20 μL denaturing buffer containing 1 M urea, 4 % SDS and 4 % β-mercaptoethanol.…”

Section: Fibrinogenolytic Activitymentioning

confidence: 99%

Jackfruit (Artocarpus heterophyllus) seed extract exhibits fibrino(geno)lytic activity

Gangaraju¹,

Manjappa²,

Subbaiah³

et al. 2015

Phcog J

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Objective: The current study assesses the fibrinogen and fibrin clot hydrolyzing activities of aqueous seed extract of Jackfruit (AqSEJ). Methods: The protein banding pattern of AqSEJ (100 μg) was analyzed on SDS-PAGE. The proteolytic activity of AqSEJ was confirmed by spectrophotometer and zymography experiments. Fibrinogen, fibrin and plasma protein hydrolyzing activities of AqSEJ were analyzed on SDS-PAGE under reduced conditions. Plasminogen activation and indirect hemolytic activities was analyzed using spectrophotometer. The non-toxic property of AqSEJ was tested by edema, hemorrhage in experimental mice. Results: AqSEJ exhibited proteolytic activity and the specific activity was found to be 1.04 units/mg/min. Furthermore, AqSEJ non-specifically hydrolyzed Aα, followed by Bβ and γ chains of human fibrinogen and specifically hydrolyzed α polymer and α chain of partially cross linked human fibrin clot without affecting β chain and γ-γ dimer even up to the tested dose of 30 μg for the incubation period of 8 hours. Importantly, AqSEJ did not hydrolyze other plasma proteins and devoid of plasminogen activation property. The proteolytic activity of AqSEJ was completely neutralized by PMSF and IAA, while EDTA, EGTA, 1,10-Phenanthroline did not, suggesting the presence of serine and cysteine family proteases. Moreover, AqSEJ did not cause edema and hemorrhage in experimental mice up to the tested dose of 200 μg and non-toxic to RBC cells. Conclusion: AqSEJ hydrolyzes fibrinogen and fibrin clot and non-toxic in nature. Hence, this work showcases the potential applications of Jack fruit seed proteases in the treatment of thrombotic disorders.

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“…Fibrinogenolytic activity was assayed according to the method of Ouyang and Teng (1976). Human fibrinogen (50 μg) was incubated with Malabarase in 10 mM Tris-HCl buffer pH 7.4 for different time intervals at 37°C.…”

Section: Fibrinogenolytic Activity Of Malabarasementioning

confidence: 99%

Malabarase, a serine protease with anticoagulant activity from Trimeresurus malabaricus venom

Kumar

Manjunath

Joshi

et al. 2013

Comparative Biochemistry and Physiology Part B: Biochemistry an

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Fibrinogenolytic enzymes of Trimeresurus mucrosquamatus venom

Cited by 218 publications

References 22 publications

Anti-hemolytic and Anti-cytotoxic Effect of Two Artemisia Species (A. campestris and A. herba-alba) Essential Oil against Snake Venom

Anti-hemolytic and Anti-cytotoxic Effect of Two Artemisia Species (A. campestris and A. herba-alba) Essential Oil against Snake Venom

Jackfruit (Artocarpus heterophyllus) seed extract exhibits fibrino(geno)lytic activity

Malabarase, a serine protease with anticoagulant activity from Trimeresurus malabaricus venom

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