Fibrinogen Increases Resveratrol Solubility and Prevents it from Oxidation

Abstract

The French paradox describes a lower incidence of cardiovascular problems despite a high intake of saturated fats. This phenomenon was associated with higher consumption of red wine, as it was later discovered that the presence of antioxidants, including resveratrol, have beneficial effects. We hypothesized that resveratrol may have a more direct role in protection from harmful oxidation, presumably through binding to important proteins of the blood coagulation process. Spectrofluorimetry demonstrated that resveratrol is capable of binding to fibrinogen, the main protein in the coagulation process, which is also important as a food additive. Various spectroscopic methods determined that binding does not cause fibrinogen unfolding or destabilization since protein melting temperature remains unchanged. A mutually protective effect against the free radical-induced oxidation of polyphenol and fibrinogen was found. The presence of fibrinogen caused only a negligible masking effect of the antioxidative abilities of resveratrol, measured by a reduction of hexacyanoferrate (III), while greatly increasing its solubility in an aqueous environment, thus increasing its potential bioavailability. Due to its interaction with fibrinogen, resveratrol may serve as an antioxidant at the site of injury. The antioxidative effect of resveratrol may also protect and thus keep the desired characteristics of fibrinogen during the application of this protein as a food additive.

Unraveling the Antioxidant, Binding and Health-Protecting Properties of Phenolic Compounds of Beers with Main Human Serum Proteins: In Vitro and In Silico Approaches

“…These results are in agreement with the data reported in [34], where the efficiency of flavonoids as free radical scavengers was proved. The obtained results (Table 3) on quenching of fibrinogen with investigated samples (12.5-18.1%) are in line with other reports, where the absorption peak at about 351 nm was measured at the interaction of resveratrol, and the fluorescence intensity exhibited a decrease [35][36][37]. The comparison of the obtained results of quenching of HSA with the investigated samples showed about 1.7 times higher quenching than with fibrinogen, especially with flavonoids, and this is in agreement with a recent report [37].…”

“…The obtained results (Table 3) on quenching of fibrinogen with investigated samples (12.5-18.1%) are in line with other reports, where the absorption peak at about 351 nm was measured at the interaction of resveratrol, and the fluorescence intensity exhibited a decrease [35][36][37]. The comparison of the obtained results of quenching of HSA with the investigated samples showed about 1.7 times higher quenching than with fibrinogen, especially with flavonoids, and this is in agreement with a recent report [37]. As it was shown in Tables 1-3, there is a correlation between polyphenols, antioxidant activities and binding properties of the investigated beer samples.…”

Role of Resveratrol in Prevention and Control of Cardiovascular Disorders and Cardiovascular Complications Related to COVID-19 Disease: Mode of Action and Approaches Explored to Increase Its Bioavailability

“…Human fibrinogen is also shown to bind and protect resveratrol [ 69 ] and mutual oxidative protection of both partners was observed in the complex of resveratrol bound to fibrinogen. Therefore, fibrinogen may prolong the activity of resveratrol and release it unaltered from the complex at the site of injury thus enabling it to exert a positive effect on a healing process.…”

“…Fibrinogen is the most susceptible plasma protein to oxidation [ 136 ] and it is generally agreed that oxidation of fibrinogen has significant impact on its function [ 137 , 138 , 139 ]. By binding to small bioactive molecules that have antioxidative properties, fibrinogen can be protected from harmful oxidation, and therefore maintain its normal function [ 69 , 140 ]. The prevention mechanism of thrombosis by resveratrol includes its inhibitory activity on blood platelet activation, as increased activation of platelets accompanies inflammation and oxidative stress [ 141 ].…”

Role of Resveratrol in Prevention and Control of Cardiovascular Disorders and Cardiovascular Complications Related to Covid-19 Disease: Mode of Action and Approaches Explored to Increase Its Bioavailability

“…Fibrinogen is the most susceptible plasma protein to oxidation [130] and it is generally agreed that oxidation of fibrinogen has significant impact on its function [131][132][133]. By binding to small bioactive molecules that have antioxidative properties, fibrinogen can be protected from harmful oxidation, and therefore maintain its normal function [69,134]. The prevention mechanism of thrombosis by resveratrol includes its inhibitory activity on blood platelets activation, as increased activation of platelets accompanies inflammation and oxidative stress [135].…”