Fibrinogen Fucosylation as a Prognostic Marker of End-Stage Renal Disease in Patients on Peritoneal Dialysis

Abstract

Glycosylation may strongly affect protein structure and functions. A high risk of cardiovascular complications seen in patients with end-stage renal disease (ESRD) is, at least partly associated with delayed clot formation, increased clot strength, and delayed cloth lysis. Taking into consideration that fibrinogen mediates these processes, we isolated fibrinogen from the plasma from patients with ESRD on peritoneal dialysis (ESRD-PD), and examined glycosylation of native fibrinogen and its subunits by lectin-based microarray and lectin blotting. Compared to healthy controls, fibrinogen from patients had increased levels of A2BG2 and decreased levels of FA2 glycan. The distribution of glycans on individual chains was also affected, with the γ chain, responsible for physiological functions of fibrinogen (such as coagulation and platelet aggregation), being most prone to these alterations. Increased levels of multi-antennary N-glycans in ESRD-PD patients were also associated with the type of dialysis solutions, whereas an increase in the fucosylation levels was strongly related to the peritoneal membrane damage. Consequently, investigation of fibrinogen glycans can offer better insight into fibrinogen-related complications observed in ESRD-PD patients and, additionally, contribute to prognosis, choice of personalised therapy, determination of peritoneal membrane damage, and the length of utilization of peritoneum for dialysis.

Nanoparticle Biomolecular Corona-Based Enrichment of Plasma Glycoproteins for N-Glycan Profiling and Application in Biomarker Discovery

“…Protein precipitation and affinity methods have been used for fibrinogen isolation from human plasma to investigate its N-glycosylation in association with diseases. , The former exploits the low solubility of fibrinogen (among the major plasma proteins) and depends on the dehydration of proteins, which likely results in the coprecipitation of fibrinogen with different proteins, particularly albumin . Both protein precipitation and corona enrichment are not as selective as the affinity method; however, the major advantage of the corona enrichment method for glycan profiling compared to the precipitation method is that it could be utilized in different contexts, for different corona protein enrichment by changing the surface chemistry or plasma protein/NP ratio.…”

Higher D‐dimer and activated partial thromboplastin time are poor prognostic factors in patients with peritoneal dialysis‐related peritonitis

“…DD was higher in patients with CAPD than normal volunteers [16]. PD patients have higher fibrinogen concentrations than HD patients because glucose‐based dialysate produces glycation end products [6, 17]. DD is especially higher in patients with PD [18].…”

Community acquired pneumonia: CAP

“…Više od 90% svih smrtnih ishoda se dešava u poznim godinama (gerijatrijska populacija), a češća je kod imunokompromitovanih osoba (primarne ili sekundarne imunodeficijencije) ili kod transplantiranih bolesnika zbog primene imunosupresivne terapija, kao i kod bolesnika u stanjima hronične inflamacije, kao što su bolesnici lečeni različitim hemioterapeuticima ili različitim metodama zamene bubrežne funkcije eng. Renal Replacement Therapy [6,7].…”