Fibrinogen

Marbet, G A; Duckert, F

doi:10.1007/978-94-011-2992-3_6

Cited by 8 publications

(15 citation statements)

References 32 publications

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“…chain by thrombin is about 7mmol/ L, while the normal plasma concentration is approximately 9 mmol/L; therefore the velocity of clot formation is signi¢cantly a¡ected by di¡erent functional ¢brinogen concentrations even within the physiological range. 21 The plasma dilution reduces the in£u-ence of endogenous and exogenous inhibitors (e.g. antithrombins, heparinoids) but not the retarding e¡ects of FDP (see above).…”

Section: Methodology Principlesmentioning

confidence: 99%

“…21,46 Principle of measurement. Thrombin is added to citrated plasma and during prolonged incubation (in the absence of Ca 2+ ions) converts all ¢brinogen to a ¢brin clot; epsilon aminocaproic acid (EACA) is added to inhibit plasmin degradation of the ¢brin clot (see Fig.…”

Section: Methodology Principlesmentioning

confidence: 99%

“…The ¢brinogen concentration in g/L can be measured either by tyrosine determination in the well washed clot or more easily by measuring light absorbance at 282 nm. Materials and assay procedures are given in reference 21. Clotting rate assay 20,21 Principle of measurement. A standardized, high concentration (*100 U/mL) thrombin solution is added to bu¡er-diluted plasma and the clotting time recorded.…”

Section: Methodology Principlesmentioning

confidence: 99%

“…Second, clottable ¢brinogen is believed to be the most clinically relevant measure, given the key function of ¢brinogen in achieving secure secondary haemostasis. For routine investigation, monitoring and treatment of bleeding risk, automated clotting rate assays 20,21 which measure the clotting time of plasma dilutions are currently preferred for quantitation of low functional (clottable) ¢brinogen assays. 13 Following the development of automated coagulometers which employ optical density or light scatter to detect ¢brin formation as the endpoint in clotting time assays, many haematology laboratories have estimated ¢brinogen concentrations from clotting times, notably the prothrombin time (PT; PT-derived ¢brinogen assay).…”

Section: Bleeding Riskmentioning

confidence: 99%

“…The automated clotting rate assay 20,21 for assessment of bleeding risk associated with low clottable plasma ¢brinogen, and for assessment of risk for arterial disease events; . An immunoassay (ELISA or nephelometric) to distinguish hypo-from dys-¢brinogenaemias; .…”

Section: ^48mentioning

confidence: 99%

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Plasma fibrinogen

2004

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Fibrinogen is the major plasma protein coagulation factor. Low plasma fibrinogen concentrations are therefore associated with an increased risk of bleeding due to impaired primary and secondary haemostasis. Fibrinogen is a classical positive acute-phase reactant protein and is an independent predictor of coronary heart disease events. This review considers available methods for measurement of fibrinogen and makes recommendations as to their appropriate use.Total clottable fibrinogen assays are the definitive and reference method for plasma fibrinogen measurement. However, they are time-consuming and are rarely required in clinical practice. Clotting rate assays remain the routine method of choice for investigation, monitoring and treatment of bleeding disorders associated with low plasma fibrinogen concentrations. They are appropriately sited in haematology or haemostasis laboratories, with facilities for further relevant investigations, and expert advice from consultant haematologists on appropriate management. They have also been used in the majority of studies investigating increasing fibrinogen concentrations as a cardiovascular risk factor. Prothrombin-time derived assays are widely used, because they are less expensive and come at no extra cost with prothrombin time assays. However, their results vary widely with analysers and reagents, show discrepancies with clotting rate assays for both low and normal plasma fibrinogen samples, and at present they are not recommended by routine clinical use. Immunoassays (radial immunodiffusion, enzyme-linked immunosorbent assay or nephelometric) are useful in (a) differentiating hypofibrinogenaemia from dysfibrinogenaemia, and (b) assessing cardiovascular risk and acute-phase reactions. Unlike clottable fibrinogen assays, immunoassays can be performed in dipotassium edetate anticoagulated samples.

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Section: Methodology Principlesmentioning

confidence: 99%

Section: Methodology Principlesmentioning

confidence: 99%

Section: Methodology Principlesmentioning

confidence: 99%

Section: Bleeding Riskmentioning

confidence: 99%

Section: ^48mentioning

confidence: 99%

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Plasma fibrinogen

2004

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Fibrinolytic risk factor clustering and insulin resistance in healthy male relatives of men with intermittent claudication

Parry

Grant

Scott

2006

British Journal of Surgery

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Soluble vascular cell adhesion molecule-1 and E-selectin levels in relation to vascular risk factors and to E-selectin genotype in the first degree relatives of NIDDM patients and in NIDDM patients

1998

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The first degree relatives of non-insulin-dependent diabetes mellitus (NIDDM) patients are at increased risk of coronary artery disease and also have a lifetime risk of NIDDM of up to 40 % [1]. The development of these conditions appears to be predicted by the presence of features of insulin resistance and associated clustering of atherosclerotic risk in these subjects [2].Adhesion molecules are expressed on the cellular surface of endothelium in response to inflammatory stimuli [3] and can also be detected in soluble form in the circulation. The cellular adhesion molecules E-selectin and vascular cell adhesion molecule-1 (VCAM-1) are thought to play a role in the early stages of vascular disease by facilitating the attachment of leucocytes to, and the transmigration of leucocytes through, the endothelium [4]. It is possible that circulating forms of these adhesion molecules may also have functional importance [5]. The source Diabetologia (1998) Summary To investigate the metabolic and genetic associations of levels of soluble adhesion molecules, plasma levels of soluble E-selectin and vascular cell adhesion molecule-1 were measured in 60 non-insulin-dependent diabetes mellitus (NIDDM) patients, 60 first-degree relatives of NIDDM patients and 60 control subjects, none of whom displayed clinical features of vascular disease. In addition, E-selectin A561C genotype, coding for a serine to arginine change, was determined. E-selectin levels were elevated in the patient group; 57 [52±63] (mean [95 % confidence intervals]) ng/ml, compared with both relatives; 44 [39±50] ng/ml p = 0.001 and controls 39.5 [36±43] ng/ml p = 0.0001. E-selectin levels correlated with triglycerides, tissue-plasminogen activator and plasminogen activator inhibitor-1 activity in all groups. Levels of E-selectin were related to E-selectin genotype, being higher in subjects possessing the arginine allele (51.4 vs 44.5 ng/ml p < 0.05). E-selectin levels were higher in males than females in controls (

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Fibrinogen

Cited by 8 publications

References 32 publications

Plasma fibrinogen

Plasma fibrinogen

Fibrinolytic risk factor clustering and insulin resistance in healthy male relatives of men with intermittent claudication

Soluble vascular cell adhesion molecule-1 and E-selectin levels in relation to vascular risk factors and to E-selectin genotype in the first degree relatives of NIDDM patients and in NIDDM patients

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