Fibrin Substrate Supports Chondrogenesis of Adipose-Derived Stem Cells Supplemented by Human Platelet-Rich Plasma and L-Ascorbic Acid 2-Phosphate

Rosadi, Imam; Moegni, K. Fernandi; Ad, Sulaeha; Sobariah, Siti; Rosliana, Iis; Wahyuningsih, Komang Ardi; Widyastuti, Tias; Afini, Irsyah; Subroto, Wismo Reja

doi:10.1016/j.jcyt.2020.03.380

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“…The optimal concentration of ascorbic acid needs to be evaluated as a result. Fibrin substrate is a natural material substrate that is collected from human blood 16,17 . Its potency to support cell attachment plays a role in the proliferation and differentiation of cells 18 .…”

Section: Introductionmentioning

confidence: 99%

L-ascorbic acid-2-phosphate and Fibrin Substrate Enhances the Chondrogenic Differentiation of Human Adipose-derived Stem Cells

et al. 2021

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Background: Adipose-derived stem cells (ASCs) have been developed as a substitute for chondrocytes in cartilage tissue engineering. This study aimed to evaluate the potency of ascorbic acid to induce the chondrogenic differentiation of human ASCs cultured on fibrin substrate. Methods: Human ASCs were isolated by an enzymatic process and characterized. The fibrin scaffold was fabricated from human blood as a natural scaffold. The ASCs were cultured in 10% fetal bovine serum (FBS) supplemented by ascorbic acid in various concentrations (1, 3, 6 µg/ml) and a control without an ascorbic acid supplementation. The chondrogenic differentiation of the ASCs was evaluated involving glycosaminoglycan analysis, and the mRNA expression of type 2 collagen, aggrecan and type 1 collagen. Results: Chondrogenic differentiation occurred when the ASCs were supplemented by 3 µg/ml of ascorbic acid and cultured on a fibrin scaffold confirmed by glycosaminoglycan (GAG) analysis, and the mRNA expression of type 2 collagen and aggrecan. The expression of type 1 collagen, which is an osteogenic marker, was low. Conclusions: ASCs with a supplementation of 3 µg/ml of ascorbic acid cultured on a fibrin substrate promotes chondrogenic differentiation.

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Section: Introductionmentioning

confidence: 99%