Fibrin seal in wound healing: Effect of thrombin and [Ca2+] on human skin fibroblast growth and collagen production

Bioactivity and stability of endogenous fibrogenic factors in platelet‐rich fibrin

“…Moreover, this composition of the FS increased the synthesis of type I and type III collagens compared with medium alone. 38 PRF s strongly stimulated the synthesis of type I collagen in the dermal fibroblasts as observed previously for a platelet lysate formula. 24 This was unlikely attributed to endogenous PDGF-AB, the major PDGF isoform of platelets.…”

“…This indicates that the bioactivity of PRF s was primarily attributed to the platelets in our two-dimensional in vitro assay, although there are some structural differences in the fibrin in PRF s and Tisseel s . 22 In another study, Michel et al 38 observed that by lowering thrombin from 250 to 50 U/mL and the final calcium concentration from 20 to 2 mmol/L this resulted in a three-fold increase in fibroblast Table 1. Mean AE SEM (n53).…”

In vitroengineering of complete autologous oral mucosa equivalents: characterization of a novel scaffold

“…The method is fast, cheap and simple, compared with synthetic fibrin (26). Fibrin is the ideal matrix because it can stimulate fibroblast proliferation (27) while preserving stratification and differentiation of keratinocytes in vivo (12). The CAOME may be removed manually, but doing so demands the use of vaseline gauze and Histoacryl ® (9).…”

Zelltransplantationen zum Liquorfistelverschluss

“…Be reits Haut fi bro blasten kul ti vie run gen in An we sen heit von Fibrinkle ber ha ben die Be fun de ei ner hohen At trak ti vi tät die ser Mat rix für Fi broblas ten ein her ge hend mit ei ner ge stei gerten Kol la gen syn the se ge zeigt und zu dem Schluss ge führt, dass Fi brinkle ber einen güns ti gen Ein fluss auf Wund hei lungs prozes se aus übt, in de nen Fi bro blas ten be deutend sind [18].…”