Fibrin promotes development and function of macaque primary follicles during encapsulated three-dimensional culture

Xu, Jing; Lawson, Maralee S.; Yeoman, Richard R.; Molskness, Theodore A.; Ting, Alison Y.; Stouffer, Richard L.; Zelinski, Mary B.

doi:10.1093/humrep/det093

Cited by 103 publications

(130 citation statements)

References 39 publications

(80 reference statements)

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“…Notably, in vitro-developed macaque follicles produced AMH with expression patterns similar to those observed in women by immunohistochemistry [11]. Media concentrations of AMH protein were detectable during the preantral stage and peaked after antrum formation [10]. The data also indicated that, although the diameters were similar at onset of culture, macaque preantral follicles differ in their ability to produce AMH.…”

Section: Introductionsupporting

confidence: 57%

“…The data also indicated that, although the diameters were similar at onset of culture, macaque preantral follicles differ in their ability to produce AMH. Media AMH concentrations correlate positively with follicle growth rates during monkey follicle culture [10]. Thus, macaque follicles grown in vitro may mimic those developed in vivo in terms of AMH production.…”

Section: Introductionmentioning

confidence: 85%

“…Animals were treated according to the National Institutes of Health's Guide for the Care and Use of Laboratory Animals. Protocols were approved by the ONPRC Institutional Animal Care and Use Committee [10].…”

Section: Animal Use and Ovary Collectionmentioning

confidence: 99%

“…An encapsulated three-dimensional culture system was developed to support rhesus macaque preantral (primary and secondary) follicle growth to the small antral stage with function in steroidogenesis and oocyte maturation [10]. Notably, in vitro-developed macaque follicles produced AMH with expression patterns similar to those observed in women by immunohistochemistry [11].…”

Section: Introductionmentioning

confidence: 99%

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Anti-Müllerian hormone is produced heterogeneously in primate preantral follicles and is a potential biomarker for follicle growth and oocyte maturation in vitro

Letaw

et al. 2016

J Assist Reprod Genet

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Purpose The main goals of this study were to investigate the expression of anti-Müllerian hormone (AMH) and its receptor (AMHR2) during follicular development in primates, and to evaluate the potential of AMH as a biomarker for follicle growth and oocyte maturation in vitro. Methods The mRNA and protein expression of AMH and AMHR2 were determined using isolated follicles and ovarian sections from rhesus macaques (n = 4) by real-time PCR and immunohistochemistry, respectively. Isolated secondary follicles were cultured individually. Follicle growth and media AMH concentrations were assessed by ELISA. The mRNA expression profiles, obtained from RNA sequencing, of in vitro-and in vivo-developed antral follicles were compared. Secondary follicles from additional animals (n = 35) were cultured. Follicle growth, oocyte maturation, and media AMH concentrations were evaluated for forecasting follicular development in vitro by AMH levels. Results AMH immunostaining was heterogeneous in the population of preantral follicles that were also stained for AMHR2. The mRNA expression profiles were comparable between in vivo-and in vitro-developed follicles. AMH levels produced by growing follicles were higher than those of nongrowing follicles in culture. With a cutoff value of 1.40 ng/ml, 85 % of nongrowing follicles could be identified while eliminating only 5 % of growing follicles. Growing follicles that generated metaphase II-stage oocytes secreted greater amounts of AMH than did those yielding immature germinal vesicle-stage oocytes. Conclusions AMH, co-expressed with AMHR2, was produced heterogeneously by preantral follicles in macaques with levels correlated positively with follicle growth and oocyte maturation. AMH may serve as a biomarker for primate follicular development in vitro.

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Section: Introductionsupporting

confidence: 57%

Section: Introductionmentioning

confidence: 85%

Section: Animal Use and Ovary Collectionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Anti-Müllerian hormone is produced heterogeneously in primate preantral follicles and is a potential biomarker for follicle growth and oocyte maturation in vitro

Letaw

et al. 2016

J Assist Reprod Genet

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“…Follicle culture at different stages of development has been used to study diverse biological processes (folliculogenesis, steroidogenesis, and oocyte maturation) in various species, such as goat [9,10], mouse [11][12][13], rat [14], sheep [15,16], dog [17][18][19], and monkey [20][21][22][23]. Regarding the domestic cat, efforts to date have focused on oocytes [7,[24][25][26][27][28][29][30] or preantral follicle cultures [19,31].…”

Section: Introductionmentioning

confidence: 99%

Felis catus ovary as a model to study follicle biology in vitro

Rojo

Linari

Musse

et al. 2015

J Assist Reprod Genet

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Purpose The current study was designed to evaluate the response of individual intact antral follicles from adult female domestic cats to a luteinizing hormone (LH) stimulus in vitro by assessing cumulus-oocyte expansion (C-OE) and steroid production. Methods C-OE and steroid levels (estradiol [E2] and progesterone [P4]) obtained from individual antral feline follicles (n=366 follicles; n=56 cats) were analyzed after 12 or 24 h of culture in the presence or absence of LH (low [3.4 ng/ml] or high [100 ng/ml]). Results At the end of the culture, the highest percentage of expanded cumulus-oocyte complexes (COCs) was observed in the LH groups at 12 or 24 h in comparison to their controls (p<0.001). There was a significant increase in expanded COCs when comparing LH concentrations (high vs. low) at 12 or 24 h. Higher levels of both E2 and P4 were observed in the media from antral follicles after 12 and 24 h of culture in the presence of LH (both concentration, p<0.05). There was no association between hormone levels and follicle diameter; high variability was observed in the steroid levels produced by antral follicles within all treatment groups.Conclusions These data indicate, for the first time, that feline antral follicles (0.5-2 mm) from different stages of the natural estrous cycle can be cultured and will respond to an LH stimulus, based on an increase in steroid levels as well as C-OE after 12 or 24 h in culture.

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Supplemented αMEM/F12‐based medium enables the survival and growth of primary ovarian follicles encapsulated in alginate hydrogels

Tagler

Makanji

Anderson

et al. 2013

Biotech & Bioengineering

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Hydrogel-encapsulating culture systems for ovarian follicles support the in vitro growth of secondary follicles from various species including mouse, non-primate human, and human; however, the growth of early stage follicles (primary and primordial) has been limited. While encapsulation maintains the structure of early stage follicles, feeder cell populations, such as mouse embryonic fibroblasts (MEFs), are required to stimulate growth and development. Hence, in this report, we investigated feeder-free culture environments for early stage follicle development. Mouse ovarian follicles were encapsulated within alginate hydrogels and cultured in various growth medium formulations. Initial studies employed embryonic stem cell medium formulations as a tool to identify factors that influence the survival, growth, and meiotic competence of early stage follicles. The medium formulation that maximized survival and growth was identified as αMEM/F12 supplemented with fetuin, insulin, transferrin, selenium, and follicle stimulating hormone (FSH). This medium stimulated the growth of late primary (average initial diameter of 80 µm) and early secondary (average initial diameter of 90 µm) follicles, which developed antral cavities and increased to terminal diameters exceeding 300 µm in 14 days. Survival ranged from 18% for 80 µm follicles to 36% for 90 µm follicles. Furthermore, 80% of the oocytes from surviving follicles with an initial diameter of 90–100 µm underwent germinal vesicle breakdown (GVBD), and the percentage of metaphase II (MII) eggs was 50%. Follicle/oocyte growth and GVBD/MII rates were not significantly different from MEF co-culture. Survival was reduced relative to MEF co-culture, yet substantially increased relative to the control medium that had been previously used for secondary follicles. Continued development of culture medium could enable mechanistic studies of early stage folliculogenesis and emerging strategies for fertility preservation.

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Fibrin promotes development and function of macaque primary follicles during encapsulated three-dimensional culture

Cited by 103 publications

References 39 publications

Anti-Müllerian hormone is produced heterogeneously in primate preantral follicles and is a potential biomarker for follicle growth and oocyte maturation in vitro

Anti-Müllerian hormone is produced heterogeneously in primate preantral follicles and is a potential biomarker for follicle growth and oocyte maturation in vitro

Felis catus ovary as a model to study follicle biology in vitro

Supplemented αMEM/F12‐based medium enables the survival and growth of primary ovarian follicles encapsulated in alginate hydrogels

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