Fibrin Encapsulation and Vascular Endothelial Growth Factor Delivery Promotes Ovarian Graft Survival in Mice

Shikanov, Ariella; Zhang, Zheng; Xu, Min; Smith, Rachel M.; Rajan, Aniruddha; Woodruff, Teresa K.; Shea, Lonnie D.

doi:10.1089/ten.tea.2011.0204

Cited by 119 publications

(105 citation statements)

References 46 publications

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“…Although VEGF and bFGF augment the neovascularization effect especially under hypoxia, we still have to face the problem of the short half-life of these factors. With the development of tissue engineering [27,40], the beneficial effect of angiogenic factors on further follicular development or fertility restoration should be confirmed.…”

Section: Discussionmentioning

confidence: 99%

VEGF and bFGF increase survival of xenografted human ovarian tissue in an experimental rabbit model

Wang

Ying

OuYang

et al. 2013

J Assist Reprod Genet

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Section: Discussionmentioning

confidence: 99%

VEGF and bFGF increase survival of xenografted human ovarian tissue in an experimental rabbit model

Wang

Ying

OuYang

et al. 2013

J Assist Reprod Genet

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“…In a) one primordial and one primary follicle not affected after treatment (experimental group R.T./2 h); b) primary follicles not affected by treatment (experimental 37°C/2 h). In c) one primary follicle damaged (red arrow) and one not affected (blue arrow) after treatment (experimental group 37°C/2 h) embedded with vascular growth factor [18] or the addition of different vascular endothelial growth factor isoforms to the grafts to improve angiogenesis of ovarian tissue xenotransplantation, the well preserved follicles shall have enough blood supply to survive and support natural conception [9,14]. Further work is underway to test this possibility in an improved version of the metal container for vitrification of bovine ovarian tissue.…”

Section: Discussionmentioning

confidence: 99%

The use of a metal container for vitrification of mouse ovaries, as a clinical grade model for human ovarian tissue cryopreservation, after different times and temperatures of transport

Bös-Mikich

Marques

Rodrigues

et al. 2012

J Assist Reprod Genet

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Purpose Cryopreservation of ovarian tissue is paramount for fertility preservation, with important clinical applications, especially for women suffering from an oncological condition. Several cryopreservation methodologies have been tried in search of better outcomes, especially in terms of primor-dial and primary follicles integrity post-cryopreservation. Vitrification has successfully been applied to ovarian tissue using different carriers for tissue exposure to the liquid nitrogen (LN2). Methods We developed an enclosed metal vessel, which has the advantage of a faster heat transfer, when in contact with LN2 avoiding at the same time, the direct contact with tissue. Additionally, we assessed the effect of different times and temperatures of transport between the collection of mouse ovaries and the beginning of cryopreservation, on follicular morphology after vitrification.Results Our results suggest that 37°C and R.T. help to maintain normal primordial and primary follicle morphology for up to 4 hrs after collection and beginning of vitrification in a metal container. Conclusion These data show that the metal container is an appropriate carrier for mouse ovary vitrification. The rate of morphologically normal primordial follicles up to 4 hrs.

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“…Also, delivery of VEGF on the fibrin-encapsulated follicle promotes twice as many surviving primordial follicles and an increased number of blood vessels than that of the nontreated. 24 In addition, estrogen was found to be upregulated in both VEGF expression and early follicle growth in the rodent ovary. 25 Endothelial progenitor cells (EPCs), which also promote angiogenesis, play a major role in the revascularization of injured tissue and the process of tissue repair.…”

Section: Introductionmentioning

confidence: 98%

Effect of Human Endothelial Progenitor Cell (EPC)- or Mouse Vascular Endothelial Growth Factor-Derived Vessel Formation on the Survival of Vitrified/Warmed Mouse Ovarian Grafts

et al. 2014

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The aim of this study was to evaluate the effectiveness of improving angiogenesis at graft sites on the survival of follicles in transplanted ovarian tissue. Matrigel containing 5 Â 10 5 of cord blood-derived endothelial progenitor cells (EPCs) or 200 ng of mouse vascular endothelial growth factor (VEGF) was injected subcutaneously into BALB/c-Nu mice. After 1 week, vitrified/ warmed ovaries from female B6D2F1 mice were subcutaneously transplanted into the injection sites. After 1, 2, and 4 weeks posttransplantation, the ovaries were recovered and subjected to histological analysis. Oocytes were collected from the transplanted ovaries, and their fertilization, embryonic development, and delivery were also observed. Vitrified/warmed ovaries transplanted into EPC-or VEGF-treated sites developed more blood vessels and showed better follicle survival than those transplanted into sham-injected sites. Normal embryonic development and consequent live births were obtained using oocytes recovered from cryopreserved/transplanted ovaries. Treatment with EPCs or VEGF could prevent the ischemic damage during the early revascularization stage of ovarian transplantation.

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Fibrin Encapsulation and Vascular Endothelial Growth Factor Delivery Promotes Ovarian Graft Survival in Mice

Cited by 119 publications

References 46 publications

VEGF and bFGF increase survival of xenografted human ovarian tissue in an experimental rabbit model

VEGF and bFGF increase survival of xenografted human ovarian tissue in an experimental rabbit model

The use of a metal container for vitrification of mouse ovaries, as a clinical grade model for human ovarian tissue cryopreservation, after different times and temperatures of transport

Effect of Human Endothelial Progenitor Cell (EPC)- or Mouse Vascular Endothelial Growth Factor-Derived Vessel Formation on the Survival of Vitrified/Warmed Mouse Ovarian Grafts

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