Fibrin as a cell carrier in cardiovascular tissue engineering applications

Mol, Anita; Lieshout, Marjolein I. van; Veen, Christa G. Dam-de; Neuenschwander, Stefan; Hoerstrup, Simon P.; Baaijens, F.P.T.; Bouten, Cvc Carlijn

doi:10.1016/j.biomaterials.2004.08.007

Cited by 242 publications

(193 citation statements)

References 32 publications

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“…In the presence of mammalian cells, however, fibrin can degrade rapidly due to the localised secretion of proteolytic enzymes (Ye et al 2000). To overcome this problem fibrin degradation inhibitors and fibrin stabilisers, such as aprotinin, factor XIII and e-amino-n-caproic acid, have been added to the gels to maintain the structure over longer time periods (Mol et al 2005;Park et al 2005;Ye et al 2000). Furthermore, the importance of sufficient thrombin and calcium to prevent excessive degradation has been identified (Eyrich et al 2007).…”

Section: Fibrinmentioning

confidence: 99%

Cell encapsulation using biopolymer gels for regenerative medicine

2010

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To cite this version:Nicola C. Hunt, Liam M. Grover. Cell encapsulation using biopolymer gels for regenerative medicine. Biotechnology Letters, Springer Verlag, 2010, 32 (6), pp.733-742. <10.1007/s10529-010-0221-0>. Abstract There has been a consistent increase in the mean life expectancy of the population of the developed world over the past century. Healthy life expectancy, however, has not increased concurrently. As a result we are living a larger proportion of our lives in poor health and there is a growing demand for the replacement of diseased and damaged tissues. While traditionally tissue grafts have functioned well for this purpose, the demand for tissue grafts now exceeds the supply. For this reason, research in regenerative medicine is rapidly expanding to cope with this new demand. There is now a trend towards supplying cells with a material in order to expedite the tissue healing process. Hydrogel encapsulation provides cells with a three dimensional environment similar to that experienced in vivo and therefore may allow the maintenance of normal cellular function in order to produce tissues similar to those found in the body. In this review we discuss biopolymeric gels that have been used for the encapsulation of mammalian cells for tissue engineering applications as well as a brief overview of cell encapsulation for therapeutic protein production. This review focuses on agarose, alginate, collagen, fibrin, hyaluronic acid and gelatin since they are widely used for cell encapsulation. The literature on the regeneration of cartilage, bone, ligament, tendon, skin, blood vessels and neural tissues using these materials has been summarised.

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Section: Fibrinmentioning

confidence: 99%

Cell encapsulation using biopolymer gels for regenerative medicine

2010

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“…The linear dependency of the partial scaffold volume fraction, φ s (γ ), indicates that the contact guidance by the scaffold fibers plays a major role in the distribution of the developing collagen fibers. φ max is the maximum collagen volume fraction, which is set 0.15 [estimated as possible collagen volume fraction after 2 weeks of tissue culture from the work of Van Geemen (2012) and Mol (2005)], and φ c is the current total collagen volume fraction. The exponential of β s indicates that the scaffold anisotropy influences the amount of collagen that is produced (Lee 2005;Teh et al 2013).…”

Section: Microscale Growth and Degradation Modelmentioning

confidence: 99%

“…Myofibroblasts were seeded into the scaffolds using fibrin, to prevent cells to fall through the scaffold. Briefly, bovine fibrinogen (Sigma, St. Louis, MO, USA) and bovine thrombin (Sigma) were combined to produce final construct concentrations of 10 mg/ml fibrinogen, 10 IU/ml thrombin and 15 × 106 cells/ml, based on our methods for cardiovascular tissue engineering (TE) (Mol et al 2005). Suspensions were incubated for 30 min at 37 • C in a humidified 95/5 % air/CO2 incubator to allow gelation before culture medium was added.…”

Section: Engineered Constructsmentioning

confidence: 99%

Modeling the impact of scaffold architecture and mechanical loading on collagen turnover in engineered cardiovascular tissues

Argento

Jonge

Söntjens

et al. 2014

Biomech Model Mechanobiol

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“…Sterilization was achieved by 70% ethanol incubation for 30 min. Cells were seeded at passage 7 with a seeding density of 15 million cells per cm 3 using fibrin as a cell carrier (Mol et al 2004). The TE constructs were cultured for 4 weeks.…”

Section: Tissue Culturementioning

confidence: 99%

Passive and active contributions to generated force and retraction in heart valve tissue engineering

Vlimmeren

Driessen-Mol

Oomens

et al. 2012

Biomech Model Mechanobiol

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In tissue engineered heart valves, cell-mediated stress development during culture results in leaflet retraction at time of implantation. This tissue retraction is partly active due to traction forces exerted by the cells and partly passive due to release of residual stress in the extracellular matrix and the cells. Within this study, we unraveled the passive and active contributions of cells and matrix to generated force and retraction in engineered heart valve tissues. Tissue engineered rectangular strips, fabricated from PGA/P4HB scaffolds and seeded with human myofibroblasts, were cultured for 4 weeks, after which the cellular contribution was changed at different levels. Elimination of the active cellular traction forces was achieved with Cytochalasin D and inhibition of the Rho-associated kinase pathway. Both active and passive cellular contributions were eliminated by lysation and/or decellularization of the tissue. Maximum cell activity was reached by increasing the fetal bovine serum concentration to 50%. The generated force decreased ∼20% after elimination of the active cellular component, ∼25% when the passive cellular component was removed as well and remained unaffected by increased serum concentrations. Passive retraction accounted for ∼60% of total retraction, of which ∼15% was residual stress in the matrix and ∼45% was passive cell retraction. Cell traction forces accounted for the remainder ∼40% of the retraction. Full activation of the cells increased retraction by ∼45%. These results illustrate the importance of the cells in the process of tissue retraction, not only actively retracting the tissue, but also in a passive manner to a large extent.

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Fibrin as a cell carrier in cardiovascular tissue engineering applications

Cited by 242 publications

References 32 publications

Cell encapsulation using biopolymer gels for regenerative medicine

Cell encapsulation using biopolymer gels for regenerative medicine

Modeling the impact of scaffold architecture and mechanical loading on collagen turnover in engineered cardiovascular tissues

Passive and active contributions to generated force and retraction in heart valve tissue engineering

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