Fibrin: A Versatile Scaffold for Tissue Engineering Applications

Ahmed, Tamer A.; Dare, Emma V.; Hincke, Max

doi:10.1089/teb.2007.0435

Cited by 159 publications

(216 citation statements)

References 183 publications

Supporting

Mentioning

212

Contrasting

Unclassified

Order By: Relevance

“…The suitability of fibrin as a scaffold for a variety of regenerative therapeutic applications has been reviewed (5). As early as 1993, Tisseel fibrin sealant was used to promote the engraftment of cultured keratinocytes in a nude mouse model (6).…”

Section: Discussionmentioning

confidence: 99%

Human adipose stromal vascular cell delivery in a fibrin spray

Zimmerlin¹,

Rubin²,

Pfeifer³

et al. 2013

Cytotherapy

View full text Add to dashboard Cite

Background Adipose tissue represents a practical source of autologous mesenchymal stromal cells (MSC) and vascular-endothelial progenitor cells, available for regenerative therapy without in vitro expansion. One of the problems confronting the therapeutic application of such cells is how to immobilize them at the wound site. Here, we evaluated in vitro the growth and differentiation of human adipose stromal vascular fraction (SVF) cells after delivery using a fibrin spray system. Methods SVF cells were harvested from four human adult patients undergoing elective abdominoplasty using the LipiVage™ system. After collagenase digestion, mesenchymal and endothelial progenitor cells (pericytes, supra-adventitial stromal cells, endothelial progenitors) were quantified by flow cytometry before culture. SVF cells were applied to culture vessels using the Tisseel™ fibrin spray system. SVF cell growth and differentiation was documented by immunofluorescence staining and photomicrography. Results SVF cells remained viable following application and were expanded up to three weeks, when they reached confluence and adipogenic differentiation. Under angiogenic conditions, SVF cells formed endothelial (vWF+, CD31+ and CD34+) tubules surrounded by CD146+ and α-SMA+ perivascular/stromal cells. Discussion Human adipose tissue is a rich source of autologous stem cells, which are readily available for regenerative applications such as wound healing, without in vitro expansion. Our results indicate that mesenchymal and endothelial progenitor cells, prepared in a closed system from unpassaged lipoaspirate samples, retain their growth and differentiation capacity when applied and immobilized on a substrate using a clinically approved fibrin sealant spray system.

show abstract

Section: Discussionmentioning

confidence: 99%

Human adipose stromal vascular cell delivery in a fibrin spray

Zimmerlin¹,

Rubin²,

Pfeifer³

et al. 2013

Cytotherapy

View full text Add to dashboard Cite

show abstract

“…Fibrin hydrogels have a high water content, support cell adhesion, can be used as a vehicle for cell transplantation, and possess mechanical properties similar to that of native soft tissues [1–3]. Fibrin is formed via thrombin mediated cleavage of the Aα and Bβ chains of fibrinogen followed by conformational changes and exposure of polymerization sites [1]. A 3D fibrin network, known as the fibrin clot, occurs when factor XIIIa introduces covalent bonds between lysines and glutamines between the γ-chains of the fibrin polymer [1].…”

Section: Introductionmentioning

confidence: 99%

Fibrin hydrogels for lentiviral gene delivery in vitro and in vivo

Kidd

Shin

Shea

2012

Journal of Controlled Release

View full text Add to dashboard Cite

Gene delivery from hydrogels represents a versatile approach for localized expression of tissue inductive factors than can promote cellular processes that lead to regeneration. Lentiviral gene therapy vectors were entrapped within fibrin hydrogels, either alone or complexes with hydroxylapatite (HA) nanoparticles. The inclusion of HA into the hydrogel led to the formation of small aggregates distributed throughout the hydrogel, with no obvious alteration of the pore structure outside the aggregates. The presence of HA slowed hydrogel degradation by collagenase and plasmin relative to fibrin alone, and also decreased the rate of cell migration. Lentivirus had similar release from the fibrin hydrogels formed with or without HA. The altered hydrogel properties suggest an interaction between the nanoparticle and fibrin, which may displace the virus from the particle leading to similar release profiles. Transgene expression by cells migrating into the hydrogel in vitro was reduced in the presence of HA, consistent with the role of cell migration on transgene expression. In vivo, lentivirus loaded fibrin hydrogels promoted localized transgene expression that increased through day 9 and decreased through day 14. For the fibrin only hydrogels, expression continued to decline after day 14. However, hydrogels with HA maintained this transgene expression level for an additional two weeks before declining. Immunostaining identified transgene primarily outside the fibrin-HA gel at day 9; however, at day 21, transgene expression was observed primarily within the fibrin-HA gel. The localized delivery of lentivirus provides an opportunity to enhance the bioactivity of fibrin hydrogels for a wide range of applications in regenerative medicine.

show abstract

“…Hydrogels are useful in a wide range of tissue engineering applications due to their high water content, biocompatibility, ease of cell seeding, and mechanical properties that mimic soft tissue (Lavik and Langer, 2004; Ahmed et al , 2008). Delivery of a hydrogel is also straightforward as it can be injected in liquid form and sets in situ over the course of several minutes.…”

Section: Introductionmentioning

confidence: 99%

“…The components of fibrin, fibrinogen and thrombin, can be purified from human plasma, and fibrin glue has a long history of clinical use in Food and Drug Administration (FDA) approved products such as Tisseel®, Evicel™, and Crosseal™. Additionally, fibrin has shown to be an excellent scaffold for cell delivery and tissue ingrowth in a number of tissue engineering applications (for review, see Ahmed et al ., 2008). Of specific relevance to connective tissues such as the AF, fibrin has been used to engineer muscle (Hecker et al , 2005; Huang et al , 2005; Nieponice et al , 2007; Rowe et al , 2007), skin (Hojo et al , 2003; Balestrini and Billiar, 2006;), cartilage (Passaretti et al , 2001; Connelly et al , 2004; Johnson et al , 2004; Mesa et al , 2006; Peretti et al , 2006; Eyrich et al , 2007), and connective tissue (Chong et al , 2007; Hankemeier et al , 2007).…”

Section: Introductionmentioning

confidence: 99%

Genipin-crosslinked fibrin hydrogels as a potential adhesive to augment intervertebral disc annulus repair

Schek

Michalek²,

Iatridis³

2011

eCM

118

104

View full text Add to dashboard Cite

Treatment of damaged intervertebral discs is a signifi cant clinical problem and, despite advances in the repair and replacement of the nucleus pulposus, there are few effective strategies to restore defects in the annulus fi brosus. An annular repair material should meet three specifi cations: have a modulus similar to the native annulus tissue, support the growth of disc cells, and maintain adhesion to tissue under physiological strain levels. We hypothesized that a genipin crosslinked fi brin gel could meet these requirements. Our mechanical results showed that genipin crosslinked fi brin gels could be created with a modulus in the range of native annular tissue. We also demonstrated that this material is compatible with the in vitro growth of human disc cells, when genipin:fi brin ratios were 0.25:1 or less, although cell proliferation was slower and cell morphology more rounded than for fi brin alone. Finally, lap tests were performed to evaluate adhesion between fi brin gels and pieces of annular tissue. Specimens created without genipin had poor handling properties and readily delaminated, while genipin crosslinked fi brin gels remained adhered to the tissue pieces at strains exceeding physiological levels and failed at 15-30%. This study demonstrated that genipin crosslinked fi brin gels show promise as a gap-fi lling adhesive biomaterial with tunable material properties, yet the slow cell proliferation suggests this biomaterial may be best suited as a sealant for small annulus fi brosus defects or as an adhesive to augment large annulus repairs. Future studies will evaluate degradation rate, fatigue behaviors, and long-term biocompatibility.

show abstract

Fibrin: A Versatile Scaffold for Tissue Engineering Applications

Cited by 159 publications

References 183 publications

Human adipose stromal vascular cell delivery in a fibrin spray

Human adipose stromal vascular cell delivery in a fibrin spray

Fibrin hydrogels for lentiviral gene delivery in vitro and in vivo

Genipin-crosslinked fibrin hydrogels as a potential adhesive to augment intervertebral disc annulus repair

Contact Info

Product

Resources

About