Fibrillin influence on plastid ultrastructure and pigment content in tomato fruit

Simkin, Andrew J.; Gaffé, Joël; Alcaraz, Jean-Pierre; Carde, Jean‐Pierre; Bramley, Peter M.; Fraser, Paul D.; Kuntz, Marcel

doi:10.1016/j.phytochem.2007.03.014

Cited by 147 publications

(142 citation statements)

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“…Chromoplasts sequester large amounts of carotenoids in plastoglobules or/and carotenoid storage structures of globular, tubular, fibrillar, membranous, or crystalline shapes composed of lipids and proteins (Deruere et al, 1994;Vishnevetsky et al, 1999;Simkin et al, 2007;Walter and Strack, 2011). Besides chromoplasts, all other plastid types synthesize carotenoids.…”

Section: Introductionmentioning

confidence: 99%

Carotenoid Biosynthesis in Arabidopsis: A Colorful Pathway

Ruiz-Sola

Rodríguez‐Concepción

2012

The Arabidopsis Book

458

372

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Plant carotenoids are a family of pigments that participate in light harvesting and are essential for photoprotection against excess light. Furthermore, they act as precursors for the production of apocarotenoid hormones such as abscisic acid and strigolactones. In this review, we summarize the current knowledge on the genes and enzymes of the carotenoid biosynthetic pathway (which is now almost completely elucidated) and on the regulation of carotenoid biosynthesis at both transcriptional and post-transcriptional levels. We also discuss the relevance of Arabidopsis as a model system for the study of carotenogenesis and how metabolic engineering approaches in this plant have taught important lessons for carotenoid biotechnology.

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Section: Introductionmentioning

confidence: 99%

Carotenoid Biosynthesis in Arabidopsis: A Colorful Pathway

Ruiz-Sola

Rodríguez‐Concepción

2012

The Arabidopsis Book

458

372

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“…Fibrillins are lipid-associated proteins found in all photosynthetic organisms, from cyanobacteria to plants (Pozueta-Romero et al, 1997;Kessler et al, 1999;Ytterberg et al, 2006;Simkin et al, 2007;Cunningham et al, 2010). They were first identified in fibrils, the thread-like structures involved in carotenoid storage in the chromoplasts of bell pepper (Capsicum annuum) fruit (Newman et al, 1989;Deruère et al, 1994), and were called fibrillins because of their high abundance in fibrils.…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, the overexpression of a pepper fibrillin in tobacco (Nicotiana tabacum) increased the numbers of plastoglobules (Rey et al, 2000). In addition to their structural roles, fibrillins are involved in photosynthesis during plant development, tolerance of photooxidative stress, and resistance to biotic stress (Rey et al, 2000;Leitner-Dagan et al, 2006;Simkin et al, 2007;Cunningham et al, 2010;Singh et al, 2010;Singh and McNellis, 2011). Recently, fibrillin mutants of Arabidopsis have extended our understanding of the functions of the fibrillin proteins.…”

Section: Introductionmentioning

confidence: 99%

Fibrillin 5 Is Essential for Plastoquinone-9 Biosynthesis by Binding to Solanesyl Diphosphate Synthases in Arabidopsis

2015

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Fibrillins are lipid-associated proteins in plastids and are ubiquitous in plants. They accumulate in chromoplasts and sequester carotenoids during the development of flowers and fruits. However, little is known about the functions of fibrillins in leaf tissues. Here, we identified fibrillin 5 (FBN5), which is essential for plastoquinone-9 (PQ-9) biosynthesis in Arabidopsis thaliana. Homozygous fbn5-1 mutations were seedling-lethal, and XVE:FBN5-B transgenic plants expressing low levels of FBN5-B had a slower growth rate and were smaller than wild-type plants. In chloroplasts, FBN5-B specifically interacted with solanesyl diphosphate synthases (SPSs) 1 and 2, which biosynthesize the solanesyl moiety of PQ-9. Plants containing defective FBN5-B accumulated less PQ-9 and its cyclized product, plastochromanol-8, but the levels of tocopherols were not affected. The reduced PQ-9 content of XVE:FBN5-B transgenic plants was consistent with their lower photosynthetic performance and higher levels of hydrogen peroxide under cold stress. These results indicate that FBN5-B is required for PQ-9 biosynthesis through its interaction with SPS. Our study adds FBN5 as a structural component involved in the biosynthesis of PQ-9. FBN5 binding to the hydrophobic solanesyl moiety, which is generated by SPS1 and SPS2, in FBN5-B/SPS homodimeric complexes stimulates the enzyme activity of SPS1 and SPS2.

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“…The events investigated include, in particular, the formation of protein-accumulating bodies and the remodeling of the internal membrane system. At the structural level, the differentiation of chromoplasts consists mainly of the lysis of the grana and thylakoids (Spurr and Harris, 1968) but also includes new synthesis of membranes derived from the inner plastid membrane and that become the site for the formation of carotenoids (Simkin et al, 2007). At the biochemical and molecular level chromoplast differentiation studies have been largely dedicated to the synthesis of carotenoids (Camara et al, 1995;Bramley, 2002).…”

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confidence: 99%

Proteomic Analysis of Chloroplast-to-Chromoplast Transition in Tomato Reveals Metabolic Shifts Coupled with Disrupted Thylakoid Biogenesis Machinery and Elevated Energy-Production Components

et al. 2012

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A comparative proteomic approach was performed to identify differentially expressed proteins in plastids at three stages of tomato (Solanum lycopersicum) fruit ripening (mature-green, breaker, red). Stringent curation and processing of the data from three independent replicates identified 1,932 proteins among which 1,529 were quantified by spectral counting. The quantification procedures have been subsequently validated by immunoblot analysis of six proteins representative of distinct metabolic or regulatory pathways. Among the main features of the chloroplast-to-chromoplast transition revealed by the study, chromoplastogenesis appears to be associated with major metabolic shifts: (1) strong decrease in abundance of proteins of light reactions (photosynthesis, Calvin cycle, photorespiration) and carbohydrate metabolism (starch synthesis/degradation), mostly between breaker and red stages and (2) increase in terpenoid biosynthesis (including carotenoids) and stress-response proteins (ascorbate-glutathione cycle, abiotic stress, redox, heat shock). These metabolic shifts are preceded by the accumulation of plastid-encoded acetyl Coenzyme A carboxylase D proteins accounting for the generation of a storage matrix that will accumulate carotenoids. Of particular note is the high abundance of proteins involved in providing energy and in metabolites import. Structural differentiation of the chromoplast is characterized by a sharp and continuous decrease of thylakoid proteins whereas envelope and stroma proteins remain remarkably stable. This is coincident with the disruption of the machinery for thylakoids and photosystem biogenesis (vesicular trafficking, provision of material for thylakoid biosynthesis, photosystems assembly) and the loss of the plastid division machinery. Altogether, the data provide new insights on the chromoplast differentiation process while enriching our knowledge of the plant plastid proteome.

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Fibrillin influence on plastid ultrastructure and pigment content in tomato fruit

Cited by 147 publications

References 43 publications

Carotenoid Biosynthesis in Arabidopsis: A Colorful Pathway

Carotenoid Biosynthesis in Arabidopsis: A Colorful Pathway

Fibrillin 5 Is Essential for Plastoquinone-9 Biosynthesis by Binding to Solanesyl Diphosphate Synthases in Arabidopsis

Proteomic Analysis of Chloroplast-to-Chromoplast Transition in Tomato Reveals Metabolic Shifts Coupled with Disrupted Thylakoid Biogenesis Machinery and Elevated Energy-Production Components

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