Fiber-optic triggered release of liposome in vivo: implication of personalized chemotherapy

Abstract

The aim of this research is to provide proof of principle by applying the fiber-optic triggered release of photo-thermally responsive liposomes embedded with gold nanoparticles (AuNPs) using a 200 μm fiber with 65 mW and 532 nm excitation for topical release in vivo. The tunable delivery function can be paired with an apoptosis biosensor based on the same fiber-optic configuration for providing real-time evaluation of chemotherapy efficacy in vivo to perform as a personalized chemotherapy system. The pattern of topical release triggered by laser excitation conveyed through optical fibers was monitored by the increase in fluorescence resulting from the dilution of self-quenching (75 mM) fluorescein encapsulated in liposomes. In in vitro studies (in 37°C phosphate buffer saline), the AuNP-embedded liposomes showed a more efficient triggered release (74.53%±1.63% in 40 minutes) than traditional temperature-responsive liposomes without AuNPs (14.53%±3.17%) or AuNP-liposomes without excitation (21.92%±2.08%) by spectroscopic measurements. Using the mouse xenograft studies, we first demonstrated that the encapsulation of fluorescein in liposomes resulted in a more substantial content retention (81%) in the tumor than for free fluorophores (14%) at 120 minutes after administration from in vivo fluorescence imaging. Furthermore, the preliminary results also suggested the tunable release capability of the system by demonstrating consecutive triggered releases with fiber-optic guided laser excitation.

Interfacing with Neural Activity via Femtosecond Laser Stimulation of Drug-Encapsulating Liposomal Nanostructures

“…In previous work, a new method of chemical release from liposomal nanostructures using optical stimulation was described (Wu et al, 2008; Leung and Romanowski, 2012; Nakano et al, 2014; Huang et al, 2015). Liposomal nanostructures are submicron spherical capsules comprising a lipid bilayer shell and an interior space that can be loaded with drugs.…”

A Review on Recent Advances on Stimuli Based Smart Nanomaterials for Drug Delivery and Biomedical Application

“…Additionally, increasing anti-tumor sensitivity and effectiveness by combining CDT with other treatments Metal Nanomaterials, noble metals, organic frameworks, mesoporous carbon-based Nanomaterials, etc. are some NANOMATERIAL-based nanoplatforms utilised for CDT [162,163].…”

Latest advances in combining gold nanomaterials with physical stimuli towards new responsive therapeutic and diagnostic strategies

“…NIR light has been employed to induce the release of doxorubicin from hollow AuNMs via the LSPR photothermal effect [83,84]. Also, gold nanospheres of 3-7 nm incorporated into the lipid bilayer of liposomes, have been used to fabricate photothermally responsive hybrid AuNMs capable of releasing fluorescein in a controlled manner [85]. Ma et al incorporated gold nanoshells, doxorubicin, and magnetic nanoparticles into micelles made from cholesteryl succinyl silane.…”