Fiber-Optic System for Intraoperative Study of Abdominal Organs during Minimally Invasive Surgical Interventions

Abstract

The paper presents the results of experimental measurements of endogenous fluorescence and blood perfusion in patients with pathology of the organs of hepatopancreatoduodenal area in vivo. A custom setup combining channels for fluorescence spectroscopy (excitation wavelengths of 365 nm and 450 nm) and laser Doppler flowmetry (1064 nm) with fibre optical probe for nondestructive laparoscopic measurements has been developed and applied during minimally invasive operation procedure. Preliminary measurements with two aforementioned channels have been performed at specified excitation wavelengths. The possibility of obtaining fluorescence spectra and laser Doppler flowmetry signals in vivo during minimally invasive interventions was shown. Obtained data show perspectives of further research on technical and methodological development of optical diagnostic methods for minimally invasive surgery. The obtained results can be used to provide a deeper understanding of pathological processes influence on optical properties of abdominal organs tissues, which will ultimately help surgeons to determine the state of vitality in tissues and mucous membranes directly during the process of surgical intervention.

Fluorescence Imaging System for Biological Tissues Diagnosis: Phantom and Animal Studies

“…Fluorescence diagnostic methods are based on probing the tissue with monochromatic radiation of the near ultraviolet or visible range, exciting the fluorescence of endogenous or exogenous fluorophores and recording secondary emission for further analysis. Two most popular approaches -fluorescence spectroscopy (FS) and fluorescence imaging (FI) -demonstrate significant sensitivity to the presence of pathological changes in tissues, in particular malignant ones [13][14][15]. Extensive studies have been conducted in this direction for several decades to develop methodologies and devices for effective distinguishing of normal and tumor tissues in various human organs (lungs, breast, colon, cervix, etc.)…”

Optical fine needle biopsy in hepatocellular carcinoma mouse model

“…The measurements were carried out using custom developed optical biopsy setup ( Fig. 1) implementing FS and DRS channels 14,15 . The LED with a wavelength of 365 nm and a laser diode with a wavelength of 450 nm were used to excite NADH and FAD autofluorescence.…”

Optical percutaneous needle biopsy of the liver: a pilot animal and clinical study

“…NADH and FAD are important participants in the energy metabolism of cells, and therefore monitoring of their fluorescence can detect violations in cellular metabolism. These endogenous fluorophores in cells and tissues can serve as biomarkers for studying internal differences between normal and cancer tissues 13 – 16 . Another technique, known as diffuse reflectance spectroscopy (DRS), yields information on light absorption and scattering in tissues.…”