Fiber optic nanogold-linked immunosorbent assay for rapid detection of procalcitonin at femtomolar concentration level

Chiang, Chang Yue; Huang, Tze Ta; Wang, Chih Hui; Huang, Chun Jen; Tsai, Tsung-Heng; Yu, Sung Nien; Chen, Ying Ting; Hong, Shih Wei; Hsu, Chia Wei; Chang, Ting Chou; Chau, Lai-Kwan

doi:10.1016/j.bios.2019.111871

Cited by 61 publications

(50 citation statements)

References 31 publications

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“…However, for certain diseases, the concentration of target molecules/pathogens are at extremely low level. With the adoption of our recently developed fiber optic nanogold-linked immunosorbent assay (FONLISA) in FOPPR biosensor which exhibits fM sensitivity [15], we believe that the FOPPR biosensing The accuracy of the results by the FOPPR biosensor for the analysis of HLA-B27 mRNA was compared with that by the real-time RT-PCR method using six extracted blood samples from AS patients. As shown in Figure 9b, comparison of the results by the two methods performed by linear correlation analysis shows a correlation coefficient of 0.991 and a p value < 0.0001 at the 95% level of significance, indicating that the results arising from both methods align.…”

Section: Discussionmentioning

confidence: 99%

“…However, for certain diseases, the concentration of target molecules/pathogens are at extremely low level. With the adoption of our recently developed fiber optic nanogold-linked immunosorbent assay (FONLISA) in FOPPR biosensor which exhibits fM sensitivity [15], we believe that the FOPPR biosensing equatmethodology can also be applied to the detection of SARS-CoV-2, a positive-sense single-stranded RNA (ssRNA) virus, which has resulted in the recent global COVID-19 outbreak, and will continue to broaden the application fields in molecular biology and biomedical diagnosis.…”

Section: Discussionmentioning

confidence: 99%

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Fiber Optic Particle Plasmon Resonance Biosensor for Label-Free Detection of Nucleic Acids and Its Application to HLA-B27 mRNA Detection in Patients with Ankylosing Spondylitis

Tseng

et al. 2020

Sensors

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We developed a label-free, real-time, and highly sensitive nucleic acid biosensor based on fiber optic particle plasmon resonance (FOPPR). The biosensor employs a single-strand deoxyoligonucleotides (ssDNA) probe, conjugated to immobilized gold nanoparticles on the core surface of an optical fiber. We explore the steric effects on hybridization affinity and limit of detection (LOD), by using different ssDNA probe designs and surface chemistries, including diluent molecules of different lengths in mixed self-assembled monolayers, ssDNA probes of different oligonucleotide lengths, ssDNA probes in different orientations to accommodate target oligonucleotides with a hybridization region located unevenly in the strand. Based on the optimized ssDNA probe design and surface chemistry, we achieved LOD at sub-nM level, which makes detection of target oligonucleotides as low as 1 fmol possible in the 10-μL sensor chip. Additionally, the FOPPR biosensor shows a good correlation in determining HLA-B27 mRNA, in extracted blood samples from patients with ankylosing spondylitis (AS), with the clinically accepted real-time reverse transcription-polymerase chain reaction (RT-PCR) method. The results from this fundamental study should guide the design of ssDNA probe for anti-sense sensing. Further results through application to HLA-B27 mRNA detection illustrate the feasibility in detecting various nucleic acids of chemical and biological relevance.

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Section: Discussionmentioning

confidence: 99%

“…However, for certain diseases, the concentration of target molecules/pathogens are at extremely low level. With the adoption of our recently developed fiber optic nanogold-linked immunosorbent assay (FONLISA) in FOPPR biosensor which exhibits fM sensitivity [15], we believe that the FOPPR biosensing equatmethodology can also be applied to the detection of SARS-CoV-2, a positive-sense single-stranded RNA (ssRNA) virus, which has resulted in the recent global COVID-19 outbreak, and will continue to broaden the application fields in molecular biology and biomedical diagnosis.…”

Section: Discussionmentioning

confidence: 99%

Fiber Optic Particle Plasmon Resonance Biosensor for Label-Free Detection of Nucleic Acids and Its Application to HLA-B27 mRNA Detection in Patients with Ankylosing Spondylitis

Tseng

et al. 2020

Sensors

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“…For larger and structurally complex molecules such as antibiotics, split-aptamers have not been probed as receptors but hold promise as even conventional aptasensors have allowed group-specific nanomolar detections [162]. Optical fibre-based sandwich immunosensors frequently employ AuNPs for signal enhancement which allows ultrasensitive and rapid detection [163,164]. Liu's group developed an EW immunosensor using antibody labelled fluorophores for xenoestrogens which are molecules that mimic the structure of estrogen compounds and interfere with the functions of endogenous estrogens [165].…”

Section: Evanescent Wave-based Sensors For Biomedical Diagnosticsmentioning

confidence: 99%

Recent Progress in Optical Sensors for Biomedical Diagnostics

Pirzada

Altıntaş

2020

Micromachines

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In recent years, several types of optical sensors have been probed for their aptitude in healthcare biosensing, making their applications in biomedical diagnostics a rapidly evolving subject. Optical sensors show versatility amongst different receptor types and even permit the integration of different detection mechanisms. Such conjugated sensing platforms facilitate the exploitation of their neoteric synergistic characteristics for sensor fabrication. This paper covers nearly 250 research articles since 2016 representing the emerging interest in rapid, reproducible and ultrasensitive assays in clinical analysis. Therefore, we present an elaborate review of biomedical diagnostics with the help of optical sensors working on varied principles such as surface plasmon resonance, localised surface plasmon resonance, evanescent wave fluorescence, bioluminescence and several others. These sensors are capable of investigating toxins, proteins, pathogens, disease biomarkers and whole cells in varied sensing media ranging from water to buffer to more complex environments such as serum, blood or urine. Hence, the recent trends discussed in this review hold enormous potential for the widespread use of optical sensors in early-stage disease prediction and point-of-care testing devices.

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“…Several methods have been reported for PCT determination, mainly based in the selective recognition provided by immunological interactions. These methodologies include immunoturbidimetric assays [9], chemiluminescent immunoassays [10][11][12][13][14][15][16], immunochromatographic assays [17][18][19][20][21][22], surface plasmon resonance biosensors [23][24][25], fluorescence immunosensors [26][27][28][29][30][31][32], ellipsometry immunosensors [33], colorimetric immunoassays [34][35][36], and electrochemical immunoassays [37][38][39][40][41][42][43][44][45][46][47][48][49][50][51][52][53][54][55][56]. Even differen...…”

Section: Introductionmentioning

confidence: 99%

“…In this sense, they constitute an ideal platform to perform integrated microscale immunoassays [59][60][61][62][63][64]. Despite these inherent advantages, few microfluidic immunosensors for PCT determination have been previously published, and most of them are based on optical techniques [26,[28][29][30]39].…”

Section: Introductionmentioning

confidence: 99%

Magnetic Bead-Based Electrochemical Immunoassays On-Drop and On-Chip for Procalcitonin Determination: Disposable Tools for Clinical Sepsis Diagnosis

et al. 2020

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Procalcitonin (PCT) is a known protein biomarker clinically used for the early stages of sepsis diagnosis and therapy guidance. For its reliable determination, sandwich format magnetic bead-based immunoassays with two different electrochemical detection approaches are described: (i) disposable screen-printed carbon electrodes (SPE-C, on-drop detection); (ii) electro-kinetically driven microfluidic chips with integrated Au electrodes (EMC-Au, on-chip detection). Both approaches exhibited enough sensitivity (limit of detection (LOD) of 0.1 and 0.04 ng mL−1 for SPE-C and EMC-Au, respectively; cutoff 0.5 ng mL−1), an adequate working range for the clinically relevant concentrations (0.5–1000 and 0.1–20 ng mL−1 for SPE-C and EMC-Au, respectively), and good precision (RSD < 9%), using low sample volumes (25 µL) with total assay times less than 20 min. The suitability of both approaches was successfully demonstrated by the analysis of human serum and plasma samples, for which good recoveries were obtained (89–120%). Furthermore, the EMC-Au approach enabled the easy automation of the process, constituting a reliable alternative diagnostic tool for on-site/bed-site clinical analysis.

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Fiber optic nanogold-linked immunosorbent assay for rapid detection of procalcitonin at femtomolar concentration level

Cited by 61 publications

References 31 publications

Fiber Optic Particle Plasmon Resonance Biosensor for Label-Free Detection of Nucleic Acids and Its Application to HLA-B27 mRNA Detection in Patients with Ankylosing Spondylitis

Fiber Optic Particle Plasmon Resonance Biosensor for Label-Free Detection of Nucleic Acids and Its Application to HLA-B27 mRNA Detection in Patients with Ankylosing Spondylitis

Recent Progress in Optical Sensors for Biomedical Diagnostics

Magnetic Bead-Based Electrochemical Immunoassays On-Drop and On-Chip for Procalcitonin Determination: Disposable Tools for Clinical Sepsis Diagnosis

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