Fiber optic glucose biosensor

Abstract

A fiber-optic biosensor for glucose concentration detection has been designed, based on electrostatic self-assembly. The end face of a multimode fiber is coated with poly͑allylamine hydrochloride͒ in combination with Prussian blue and the enzyme glucose oxidase. The glucose concentration that can be measured ranges between 0.06 and 2 mM. The initial velocity of change of the output biosensor signal has been found to be linear in the glucose concentration. The sensor recovers after immersion in ascorbic acid. The pH values analyzed range between 5 and 7. The sensitivity of the device has been increased by adjusting the number of bilayers and by adding some capping bilayers at the end of the structure.

Optical fiber pH sensors based on layer-by-layer electrostatic self-assembled Neutral Red

“…This technique has been successfully used for the deposition of diverse materials such as polymers, semiconductors, ceramics, metals, dyes, quantum dots, even enzymes and antibodies [4][5][6][7][8][9]. Due to this, the LbL technique is very useful for many different applications that include hydrophobic coatings, anti-corrosion protective overlays, anti reflection coatings, smart polymers for drug release, active microspheres, photovoltaic devices, OLEDs and also, sensors [10][11][12][13].…”

Coatings for Optical Fiber Sensors

“…Several authors have immobilized enzymes inside sol-gel coatings (31), in layer-by-layer thin films (85,86), or simply by physical adsorption over carbon black (87). In most of these enzyme-based sensors, there is the need to perform indirect measurements.…”

Fiber Optic pH Sensor Using Optimized Layer-by-Layer Coating Approach

“…glass, ceramics, metals, wood and plastics [5][6][7], taking also into account their varying size and topology [8,9]. In essence, the process of deposition is based on electrostatic attraction and can be used with various reagents such as polymers, nanoparticles, metals, dyes, quantum dots and nanotubes [10][11][12][13][14][15] , biomolecules such as enzymes [16] and proteins [17], etc. In this technique, a charged substrate is alternatively immersed into polyanion and polycation solutions to build up a multilayer coating.…”