Fiber-Optic Array Scanning Technology (FAST) for Detection and Molecular Characterization of Circulating Tumor Cells

Ao, Zheng; Liu, Xiaohe

doi:10.1007/978-1-4939-7144-2_20

Cited by 8 publications

(4 citation statements)

References 12 publications

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“…Automated digital microscopy (ADM) and fiber-optic array scanning technology (FAST) are cytometry-based techniques that involve the image analysis of ICC-labeled tumor cells. ADM has several disadvantages—notably, it requires an enrichment step and scans at very low rates (800 cells/s) [ 75 , 76 ]. Compared to ADM, FAST offers comparable sensitivity, greater specificity, and 500 times the scan rate while requiring no enrichment step [ 77 ].…”

Section: Current Enrichment and Detection Techniquesmentioning

confidence: 99%

“…The specificity of the FAST method was tested in the blood samples of healthy donors spiked with the colorectal cancer cell line HT-29 and showed the detection rate 1.5 × 10 −5 , and sensitivity of 98% [ 78 ]. Both FAST and ADM have proven useful for identifying very rare epithelial cells in whole blood samples after pretreatment with fluorescently labeled anti-cytokeratin antibodies [ 75 , 77 ].…”

Section: Current Enrichment and Detection Techniquesmentioning

confidence: 99%

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Recent Advances in Methods for Circulating Tumor Cell Detection

Vidlarova

Řehulková

Stejskal

et al. 2023

IJMS

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Circulating tumor cells (CTCs) are released from primary tumors and transported through the body via blood or lymphatic vessels before settling to form micrometastases under suitable conditions. Accordingly, several studies have identified CTCs as a negative prognostic factor for survival in many types of cancer. CTCs also reflect the current heterogeneity and genetic and biological state of tumors; so, their study can provide valuable insights into tumor progression, cell senescence, and cancer dormancy. Diverse methods with differing specificity, utility, costs, and sensitivity have been developed for isolating and characterizing CTCs. Additionally, novel techniques with the potential to overcome the limitations of existing ones are being developed. This primary literature review describes the current and emerging methods for enriching, detecting, isolating, and characterizing CTCs.

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Section: Current Enrichment and Detection Techniquesmentioning

confidence: 99%

Section: Current Enrichment and Detection Techniquesmentioning

confidence: 99%

Recent Advances in Methods for Circulating Tumor Cell Detection

Vidlarova

Řehulková

Stejskal

et al. 2023

IJMS

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“…FAST was originally developed to identify rare circulating cancer cells in blood with high sensitivity and specificity (Figure ). − In this application, cells preincubated with fluorescently labeled cell surface markers are plated as a monolayer on 108 × 76 mm glass slides, which are then scanned by excitation with a 488 nm laser. Emitted fluorescence is collected through a fiber-optic bundle, and the collected light is passed through a bandpass filter and analyzed by a photomultiplier tube to measure emission at 520 nm (green) and 580 nm (red/orange) to eliminate true negatives due to autofluorescence (see below).…”

Section: Resultsmentioning

confidence: 99%

Mega-High-Throughput Screening Platform for the Discovery of Biologically Relevant Sequence-Defined Non-Natural Polymers

et al. 2022

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Combinatorial methods enable the synthesis of chemical libraries on scales of millions to billions of compounds, but the ability to efficiently screen and sequence such large libraries has remained a major bottleneck for molecular discovery. We developed a novel technology for screening and sequencing libraries of synthetic molecules of up to a billion compounds in size. This platform utilizes the fiber-optic array scanning technology (FAST) to screen bead-based libraries of synthetic compounds at a rate of 5 million compounds per minute (∼83 000 Hz). This ultra-high-throughput screening platform has been used to screen libraries of synthetic “self-readable” non-natural polymers that can be sequenced at the femtomole scale by chemical fragmentation and high-resolution mass spectrometry. The versatility and throughput of the platform were demonstrated by screening two libraries of non-natural polyamide polymers with sizes of 1.77M and 1B compounds against the protein targets K-Ras, asialoglycoprotein receptor 1 (ASGPR), IL-6, IL-6 receptor (IL-6R), and TNFα. Hits with low nanomolar binding affinities were found against all targets, including competitive inhibitors of K-Ras binding to Raf and functionally active uptake ligands for ASGPR facilitating intracellular delivery of a nonglycan ligand.

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“…Using a carbohydrate microarray, the anti-tumor monoclonal antibody is tested against a large panel of carbohydrate antigens and a potential tumor glycan marker can discover in CTCs [ 43 ]. Flow cytometry and optical fiber array scanning techniques are then applied to determine whether they are the identified target tumor-specific glycan markers [ 45 ]. In another application, the tumor glycan-specific antibodies can validate using blood samples of patients with cancer for their performance in CTC-detection [ 46 ].…”

Section: Concept For Circulating Tumor Cells (Ctcs) Analysismentioning

confidence: 99%

Perspective on Cancer Therapeutics Utilizing Analysis of Circulating Tumor Cells

Jeong¹,

Kim²,

Park³

et al. 2018

Diagnostics

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Various methods are available for cancer screening, and the methods are performed depending on the origin site of cancer. Among these methods, biopsy followed by medical imaging is the most common. After cancer progression is determined, an optimal treatment—such as surgery, chemotherapy, and/or radiation therapy—is selected. A new assay has been developed that detects circulating tumor cells (CTCs). Tracking changes in CTCs may reveal important tumoral sensitivity information or resistance patterns to specific regimens and prompt changes in therapy on a personalized basis. Characterization of CTCs at the DNA, RNA, and protein levels is important for gaining insight for clinical applications. A small number of CTCs can be analyzed to obtain genome information such as the progression of cancer including metastasis, even in a single cluster. Although many clinical studies, particularly CTC enumeration and detection of specific oncogene expression, have increased the success rate of diagnosis and predicting prognosis, there is no consensus regarding the technical approaches and various aspects of the methodology, making it difficult to standardize optimal methods for CTC analysis. However, ongoing technological advances are currently being achieved and large-scale clinical studies are being conducted. Applying CTC analysis in the clinic would be very useful for advancing diagnosis, prognosis prediction, and therapeutics.

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Fiber-Optic Array Scanning Technology (FAST) for Detection and Molecular Characterization of Circulating Tumor Cells

Cited by 8 publications

References 12 publications

Recent Advances in Methods for Circulating Tumor Cell Detection

Recent Advances in Methods for Circulating Tumor Cell Detection

Mega-High-Throughput Screening Platform for the Discovery of Biologically Relevant Sequence-Defined Non-Natural Polymers

Perspective on Cancer Therapeutics Utilizing Analysis of Circulating Tumor Cells

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