Fiber degradation strategies of bacteria in rumen ecosystem

Karri, Satyanagalakshmi; Vadela, Manohar Babu; Gundi, Vijay A.K.B.

doi:10.1016/b978-0-12-821406-0.00015-1

Cited by 3 publications

(3 citation statements)

References 65 publications

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“… Terry et al (2022) observed reduced OM and NDF degradation in the A. taxiformis samples during the adaptation phase, followed by a recovery of fiber digestion in the stable phase. The reduction in fiber degradation was reflected by the significantly lower abundance of Fibrobacter , Ruminococcus , and other less common cellulolytic genera like Ruminiclostridium in A. taxiformis samples compared to the controls ( Ren et al, 2019 ; Karri et al, 2021 ). The abundance of Fibrobacter was also strongly positively correlated with CH 4 levels and negatively correlated with H 2 levels in the presence of A. taxiformis .…”

Section: Discussionmentioning

confidence: 96%

Comparative analysis of macroalgae supplementation on the rumen microbial community: Asparagopsis taxiformis inhibits major ruminal methanogenic, fibrolytic, and volatile fatty acid-producing microbes in vitro

et al. 2023

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Seaweeds have received a great deal of attention recently for their potential as methane-suppressing feed additives in ruminants. To date, Asparagopsis taxiformis has proven a potent enteric methane inhibitor, but it is a priority to identify local seaweed varieties that hold similar properties. It is essential that any methane inhibitor does not compromise the function of the rumen microbiome. In this study, we conducted an in vitro experiment using the RUSITEC system to evaluate the impact of three red seaweeds, A. taxiformis, Palmaria mollis, and Mazzaella japonica, on rumen prokaryotic communities. 16S rRNA sequencing showed that A. taxiformis had a profound effect on the microbiome, particularly on methanogens. Weighted Unifrac distances showed significant separation of A. taxiformis samples from the control and other seaweeds (p < 0.05). Neither P. mollis nor M. japonica had a substantial effect on the microbiome (p > 0.05). A. taxiformis reduced the abundance of all major archaeal species (p < 0.05), leading to an almost total disappearance of the methanogens. Prominent fiber-degrading and volatile fatty acid (VFA)-producing bacteria including Fibrobacter and Ruminococcus were also inhibited by A. taxiformis (p < 0.05), as were other genera involved in propionate production. The relative abundance of several other bacteria including Prevotella, Bifidobacterium, Succinivibrio, Ruminobacter, and unclassified Lachnospiraceae were increased by A. taxiformis suggesting that the rumen microbiome adapted to an initial perturbation. Our study provides baseline knowledge of microbial dynamics in response to seaweed feeding over an extended period and suggests that feeding A. taxiformis to cattle to reduce methane may directly, or indirectly, inhibit important fiber-degrading and VFA-producing bacteria.

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Section: Discussionmentioning

confidence: 96%

Comparative analysis of macroalgae supplementation on the rumen microbial community: Asparagopsis taxiformis inhibits major ruminal methanogenic, fibrolytic, and volatile fatty acid-producing microbes in vitro

et al. 2023

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“…Furthermore, fibre degradation is a major source of acetate in the rumen (Hua et al, 2017). Our companion study showed reduced fibre degradation within the A. taxiformis samples during the adaption phase though it had recovered by the stable phase, and this was reflected by the significantly lower abundance of Fibrobacter, Ruminococcus, and other less common cellulolytic genera like Ruminclostridium (Karri et al, 2021;Ren et al, 2019) in A. taxiformis samples compared to the controls. The abundance of Fibrobacter was also strongly positively correlated with CH 4 levels associated with A. taxiformis, and correspondingly negatively correlated with H 2 levels.…”

Section: Discussionmentioning

confidence: 68%

Comparative analysis of macroalgae supplementation on the rumen microbial community: Asparagopsis taxiformis inhibits major ruminal methanogenic, fibrolytic, and volatile fatty acid-producing microbes in vitro

O’Hara

Moote

Terry

et al. 2022

Preprint

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Seaweeds have received a great deal of attention recently for their potential as methane-suppressing feed additives in ruminants. To date, Asparagopsis taxiformis has proven a potent enteric methane inhibitor, but it is a priority to identify local seaweed varieties that may hold similar properties. It is essential that any methane inhibitor does not compromise the function of the rumen microbiome. In this study, we conducted an in vitro experiment using the RUSITEC system to evaluate the impact of A. taxiformis, Palmaria mollis, and Mazzaella japonica on rumen prokaryotic communities. 16S rRNA sequencing showed that A. taxiformis had a profound effect on the microbiome, particularly on methanogens. Weighted Unifrac distances showed significant separation of A. taxiformis samples from the control and other seaweeds (P<0.05). Neither P. mollis nor M. japonica had a substantial effect on the microbiome (P>0.05). A. taxiformis reduced the abundance of all major archaeal species (P<0.05), leading to an almost total disappearance of the methanogens. Prominent fibre-degrading and volatile fatty acid (VFA)-producing bacteria including Fibrobacter and Ruminococcus were also inhibited by A. taxiformis (P<0.05), as were other genera involved in propionate production. However, the abundance of many other major bacteria (e.g. Prevotella) was increased by A. taxiformis suggesting the rumen microbiome adapted to an initial perturbation. Our study provides baseline knowledge of microbial dynamics in response to seaweed feeding over an extended period and suggests that feeding A. taxiformis to cattle to reduce methane may directly or indirectly inhibit important fibre-degrading and VFA-producing bacteria.

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“…This difference could be explained by the differences in diets and animal species between these studies. As for fibrolytic bacteria, such as Ruminococcus [34] and Rikenel-laceae_RC9_gut_group [35], the RA of these bacteria was greater in the CON group than in the RPF group. This could be explained by the fact that the fecal fiber content was lower in the RPF group than in the CON group [30].…”

Section: Microbial Community Composition In the Fecesmentioning

confidence: 96%

The Changes in Fecal Bacterial Communities in Goats Offered Rumen-Protected Fat

Liu,

Peng,

Mao

et al. 2024

Microorganisms

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Leizhou goats are famous for their delicious meat but have inferior growth performance. There is little information on rumen-protected fat (RPF) from the Leizhou goat. Hence, we observed the effects of RPF on growth, fecal short-chain fatty acids, and bacteria community with respect to Leizhou goats. Twelve goats (13.34 ± 0.024 kg) were selected and assigned randomly to one of two treatments: (1) a control diet (CON) and (2) 2.4% RPF with a control diet (RPF). The final body weight and average daily gain (ADG) were greater (p < 0.05), and the dry matter intake (DMI): ADG was lower (p < 0.05) in the RPF group than in the CON group. There were no differences in DMI between the CON and RPF groups. The concentrations of total short-chain fatty acids, acetate, propionate, and butyrate were lower (p < 0.05) in the RPF group than in the CON group. The relative abundances of Ruminococcus, Rikenellaceae_RC9_gut_group, Treponema, norank_f__norank_o__RF39, Eubacterium_siraeum_group, and Ruminococcus_torques_group were lower (p < 0.05) in the RPF group than in the CON group. The relative abundances of Bacteroides, norank_f__norank_o__Clostridia_UCG-014, norank_f__Eubacterium_coprostanoligenes_group, Eubacterium_ruminantium_group, norank_f__Oscillospirale-UCG-010, Oscillospiraceae_UCG-002, and Family_XIII_AD3011_group were greater (p < 0.05) in the RPF group than in the CON group. It was concluded that RPF could improve the goats’ growth performance by regulating their fecal bacteria communities.

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Fiber degradation strategies of bacteria in rumen ecosystem

Cited by 3 publications

References 65 publications

Comparative analysis of macroalgae supplementation on the rumen microbial community: Asparagopsis taxiformis inhibits major ruminal methanogenic, fibrolytic, and volatile fatty acid-producing microbes in vitro

Comparative analysis of macroalgae supplementation on the rumen microbial community: Asparagopsis taxiformis inhibits major ruminal methanogenic, fibrolytic, and volatile fatty acid-producing microbes in vitro

Comparative analysis of macroalgae supplementation on the rumen microbial community: Asparagopsis taxiformis inhibits major ruminal methanogenic, fibrolytic, and volatile fatty acid-producing microbes in vitro

The Changes in Fecal Bacterial Communities in Goats Offered Rumen-Protected Fat

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