Fiber coupled, 20 MHz Repetition Rate, sub ps Ti:sapphire Laser for in vivo Nonlinear Microscopy of the Skin

Krolopp, Ádám; Fésűs, Luca; Szipőcs, Gergely; Wikonkál, Norbert; Szipöcs, R.

doi:10.1364/boda.2021.df2a.5

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“…Multispectral imaging is a promising in vivo imaging technique that uses different wavelengths to capture images, providing a map of skin chromophores including melanin and hemoglobin [20,21]. Non-linear microscopy is a novel label-free imaging method that can provide high-resolution imaging of the skin [22]. Keratins, elastin, and melanin can be visualized by two-photon absorption fluorescence (TPF), while second-harmonic generation (SHG) can be used for the imaging of collagen [23].…”

Section: Introductionmentioning

confidence: 99%

A Cross-Sectional Study of the Dermatological Manifestations of Patients with Fabry Disease and the Assessment of Angiokeratomas with Multimodal Imaging

et al. 2023

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Fabry disease (FD) is a multisystemic X-linked lysosomal storage disease that presents with angiokeratomas (AKs). Our objective was to investigate the clinical and morphologic features of AKs and to present two experimental techniques, multispectral imaging (MSI) and non-linear microscopy (NLM). A thorough dermatological examination was carried out in our 26 FD patients and dermoscopic images (n = 136) were evaluated for specific structures. MSI was used for the evaluation of AKs in seven patients. NLM was carried out to obtain histology samples of two AKs and two hemangiomas. Although AKs were the most common manifestation, the majority of patients presented an atypical distribution and appearance, which could cause a diagnostic challenge. Dermoscopy revealed lacunae (65%) and dotted vessels (56%) as the most common structures, with a whitish veil present in only 25%. Autofluorescence (405 nm) and diffuse reflectance (526 nm) images showed the underlying vasculature more prominently compared to dermoscopy. Using NLM, AKs and hemangiomas could be distinguished based on morphologic features. The clinical heterogeneity of FD can result in a diagnostic delay. Although AKs are often the first sign of FD, their presentation is diverse. A thorough dermatological examination and the evaluation of other cutaneous signs are essential for the early diagnosis of FD.

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Section: Introductionmentioning

confidence: 99%

A Cross-Sectional Study of the Dermatological Manifestations of Patients with Fabry Disease and the Assessment of Angiokeratomas with Multimodal Imaging

et al. 2023

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Fiber coupled, 20 MHz Repetition Rate, sub ps Ti:sapphire Laser for in vivo Nonlinear Microscopy of the Skin

Abstract: A fiber coupled, sub ps Ti:sapphire laser suitable for in vivo, stain free, 3D imaging of skin alterations is introduced. It is pumped by a low cost, 2.1 W pump laser and delivers 0.6 1 ps high peak power pulses optimized for fiber delivery.

Cited by 1 publication

References 5 publications

A Cross-Sectional Study of the Dermatological Manifestations of Patients with Fabry Disease and the Assessment of Angiokeratomas with Multimodal Imaging

A Cross-Sectional Study of the Dermatological Manifestations of Patients with Fabry Disease and the Assessment of Angiokeratomas with Multimodal Imaging

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