Fiber analysis: Evaluation of screen printing fabric filters bags by three statistical approaches

Marichal, M. de J.; Trujillo, A. I.; Cadenazzi, Mónica; Arias, G.

doi:10.1016/j.anifeedsci.2011.06.005

Cited by 7 publications

(8 citation statements)

References 11 publications

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“…This method is preferred by many ruminant nutritionists as it is relatively easy to perform with high‐throughput. However, in assessing fungal‐treated biomass, the accuracy of Method A greatly relies on the precision of the extraction and filtration steps . Smaller lignin and structural carbohydrates degradation products can easily be removed from the normal pore size filter bags ( Φ = 25 µm).…”

Section: Resultsmentioning

confidence: 99%

“…However, in assessing fungal-treated biomass, the accuracy of Method A greatly relies on the precision of the extraction and filtration steps. 38 Smaller lignin and structural carbohydrates degradation products can easily be removed from the normal pore size filter bags (Φ = 25 μm). Therefore, Method A should not be used as the sole method to conclude on the capability of a particular fungus in degrading different components of cell wall.…”

Section: Relationship Of Cell Wall Contents With Ruminal Digestibilitymentioning

confidence: 99%

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Evaluation of fungal degradation of wheat straw cell wall using different analytical methods from ruminant nutrition perspective

et al. 2019

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BACKGROUND White rot fungi have been used to improve the nutritive value of lignocellulose for ruminants. In feed analysis, the Van Soest method is widely used to determine the cell wall contents. To assess the reliability of this method (Method A) for determination of cell wall contents in fungal‐treated wheat straw, we compared a combined monosaccharide analysis and pyrolysis coupled to gas chromatography with mass spectrometry (Py‐GC/MS) (Method B). Ruminal digestibility, measured as in vitro gas production (IVGP), was subsequently used to examine which method explains best the effect of fungal pretreatment on the digestibility of wheat straw. RESULTS Both methods differed considerably in the mass recoveries of the individual cell wall components, which changed on how we assess their degradation characteristics. For example, Method B gave a higher degradation of lignin (61.9%), as compared to Method A (33.2%). Method A, however, showed a better correlation of IVGP with the ratio of lignin to total structural carbohydrates, as compared to Method B (Pearson's r of −0.84 versus −0.69). Nevertheless, Method B provides a more accurate quantification of lignin, reflecting its actual modification and degradation. With the information on the lignin structural features, Method B presents a substantial advantage in understanding the underlying mechanisms of lignin breakdown. Both methods, however, could not accurately quantify the cellulose contents – among others, due to interference of fungal biomass. CONCLUSION Method A only accounts for the recalcitrant residue and therefore is more suitable for evaluating ruminal digestibility. Method B allows a more accurate quantification of cell wall, required to understand and better explains the actual modification of the cell wall. The suitability of both methods, therefore, depends on their intended purposes. © 2019 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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Section: Resultsmentioning

confidence: 99%

Section: Relationship Of Cell Wall Contents With Ruminal Digestibilitymentioning

confidence: 99%

Evaluation of fungal degradation of wheat straw cell wall using different analytical methods from ruminant nutrition perspective

et al. 2019

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“…In GI powder, the contents of total sugar, reducing sugar, mannitol, total ash, total flavonoids, total triterpenoids, crude fat and crude fiber were detected using phenol-sulfuric acid assay ( 19 ), 3,5-dinitrosalicylic acid colorimetry ( 20 ), iodometry ( 21 ), regression analysis ( 22 ), the alumina colorimetric method ( 23 ), vanillin-glacial acetic acid-perchloric acid colorimetry ( 24 ), petroleum ether extraction ( 25 ) and the Ankom filter bag method ( 26 ), respectively.…”

Section: Methodsmentioning

confidence: 99%

Protective effect of Gloeostereum�incarnatum on ulcerative colitis via modulation of Nrf2/NF‑κB signaling in C57BL/6 mice

Liu

Zhang

et al. 2020

Mol Med Rep

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Chronic non-specific inflammatory cell infiltration of the colon is generally considered to be the cause of ulcerative colitis (UC). Gloeostereum incarnatum (GI), a fungus rich in amino acids and fatty acids, exhibits a variety of biological functions. In the present study, GI was identified to contain 15 fatty acids, 17 amino acids and 11 metallic elements. The protective effect of GI against UC was investigated in C57BL/6 mice with UC induced by free drinking 3.5% dextran sulfate sodium (DSS). After a 21-day oral administration, GI prevented weight loss, enhancement of the disease activity index and colonic pathological alterations in mice with UC. GI reduced the levels of pro-inflammatory factors including interleukin (IL)-1β, IL-2, IL-6 and IL-12, tumor necrosis factor α and -β, interferon α and -γ, and pro-oxidative factors including reactive oxygen species and nitric oxide. In addition, it enhanced the levels of immunological factors including immunoglobulin (Ig)A, IgM and IgG, and antioxidative factors including superoxide dismutase and catalase in the serum and/or colon tissues. GI enhanced the expression levels of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream proteins and suppressed the phosphorylation of NF-κB signaling in colon tissues. Together, GI was shown to alleviate the physiological and pathological state of DSS-induced UC in mice via its antioxidant and anti-inflammatory functions, which may be associated with its modulation of the activation of Nrf2/NF-κB signaling.

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“…In order to increase the efficiency of the quantification process, instruments have been developed in order to automate fiber analysis systems. The Filter Bag Technique (FBT) by Ankom ® is a practical example of this and has been used to the analysis of many types of feeds with different characteristics and origins (Marichal et al, 2011), and also aims the reduction of the variability associated with the operator Komarek (1993).…”

Section: Introductionmentioning

confidence: 99%

Comparative efficacy of the conventional and automated methods for determining neutral and acid detergent fiber

et al. 2016

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Different methods are available to determine fiber content in feeds. However, information about the accuracy of this methods for neutral detergent fiber (NDF) and acid detergent fiber ADF contents estimation (obtained with the use of TNT-100 nylon filtering bags) is very limited related to the large number of ruminant feed analysis. The purpose of this study was to compare the efficacy of the automated and conventional Van Soest methods to determine NDF and ADF contents for bovine cattle and feed supplements. Four classes of samples (tropical forage, maize silage hybrid, concentrated supplements and bovine cattlecattle) were evaluated for NDF and ADF contents using conventional and automated methods. Analysis involved a hierarchical factorial scheme with an entirely randomized design executed with repetitions. It was concluded that the automated method procedure generated similar results when compared to the conventional method for the determination of NDF contents in tropical forage, bovine cattle and maize silage samples, although is not recommended for samples with a high starch content. This system was not efficient for ADF determination in the evaluated samples.

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Fiber analysis: Evaluation of screen printing fabric filters bags by three statistical approaches

Cited by 7 publications

References 11 publications

Evaluation of fungal degradation of wheat straw cell wall using different analytical methods from ruminant nutrition perspective

Evaluation of fungal degradation of wheat straw cell wall using different analytical methods from ruminant nutrition perspective

Protective effect of Gloeostereum�incarnatum on ulcerative colitis via modulation of Nrf2/NF‑κB signaling in C57BL/6 mice

Comparative efficacy of the conventional and automated methods for determining neutral and acid detergent fiber

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