FIB-SEM Three-Dimensional Tomography for Characterization of Carbon-Based Materials

Nan, Nan; Wang, Jingxin

doi:10.1155/2019/8680715

Cited by 31 publications

(20 citation statements)

References 63 publications

(82 reference statements)

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“…FIB-SEM is a micromilling and analysis system that can produce 3D images of a tissue sample for characterising nanostructures and organisation [56]. FIB-SEM allows for serial milling, sectioning and imaging simultaneously [57]. The benefits of using a dual-beamed FIB-SEM system include the milling and preparation of tissue samples which are particularly fragile or porous [58].…”

Section: Fib-semmentioning

confidence: 99%

Current Update of Collagen Nanomaterials—Fabrication, Characterisation and Its Applications: A Review

Fauzi

2021

Pharmaceutics

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Tissue engineering technology is a promising alternative approach for improvement in health management. Biomaterials play a major role, acting as a provisional bioscaffold for tissue repair and regeneration. Collagen a widely studied natural component largely present in the extracellular matrix (ECM) of the human body. It provides mechanical stability with suitable elasticity and strength to various tissues, including skin, bone, tendon, cornea and others. Even though exogenous collagen is commonly used in bioscaffolds, largely in the medical and pharmaceutical fields, nano collagen is a relatively new material involved in nanotechnology with a plethora of unexplored potential. Nano collagen is a form of collagen reduced to a nanoparticulate size, which has its advantages over the common three-dimensional (3D) collagen design, primarily due to its nano-size contributing to a higher surface area-to-volume ratio, aiding in withstanding large loads with minimal tension. It can be produced through different approaches including the electrospinning technique to produce nano collagen fibres resembling natural ECM. Nano collagen can be applied in various medical fields involving bioscaffold insertion or fillers for wound healing improvement; skin, bone, vascular grafting, nerve tissue and articular cartilage regeneration as well as aiding in drug delivery and incorporation for cosmetic purposes.

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Section: Fib-semmentioning

confidence: 99%

Current Update of Collagen Nanomaterials—Fabrication, Characterisation and Its Applications: A Review

Fauzi

2021

Pharmaceutics

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“…In the dual beam system, the Ga + beam can be used for serial milling of the sample, and at the same time, a sequence of SEM cross-sectional images can be obtained. Then, segmentation and 3D visualization of the sample can be rendered using commercial software (e.g., Avizo) [78]. Such 3D tomographic analysis bridges the gap between X-ray and transmission electron microscopic tomography techniques.…”

Section: Fib and Synchrotron Techniques Synchrotron-basedmentioning

confidence: 99%

Application of FIB-SEM Techniques for the Advanced Characterization of Earth and Planetary Materials

Wang

Tang

et al. 2020

Scanning

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Advanced microanalytical techniques such as high-resolution transmission electron microscopy (HRTEM), atom probe tomography (APT), and synchrotron-based scanning transmission X-ray microscopy (STXM) enable one to characterize the structure and chemical and isotopic compositions of natural materials down towards the atomic scale. Dual focused ion beam-scanning electron microscopy (FIB-SEM) is a powerful tool for site-specific sample preparation and subsequent analysis by TEM, APT, and STXM to the highest energy and spatial resolutions. FIB-SEM also works as a stand-alone technique for three-dimensional (3D) tomography. In this review, we will outline the principles and challenges when using FIB-SEM for the advanced characterization of natural materials in the Earth and Planetary Sciences. More specifically, we aim to highlight the state-of-the-art applications of FIB-SEM using examples including (a) traditional FIB ultrathin sample preparation of small particles in the study of space weathering of lunar soil grains, (b) migration of Pb isotopes in zircons by FIB-based APT, (c) coordinated synchrotron-based STXM characterization of extraterrestrial organic material in carbonaceous chondrite, and finally (d) FIB-based 3D tomography of oil shale pores by slice and view methods. Dual beam FIB-SEM is a powerful analytical platform, the scope of which, for technological development and adaptation, is vast and exciting in the field of Earth and Planetary Sciences. For example, dual beam FIB-SEM will be a vital technique for the characterization of fine-grained asteroid and lunar samples returned to the Earth in the near future.

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“…X-ray computed tomography (X-ray CT) can provide a fairly rapid, nondestructive solution for acquiring the 3D image of the sample, if feature sizes of down to ~ 1 μm are of interest, dosing is not an issue, and the X-ray absorption level of each region of the sample is sufficient for a follow-up analysis 3 . On the other hand, for resolutions down to sub-10 nm, one can use a repetitive delayering/imaging strategy, where a scanning electron microscope (SEM) or helium ion microscope (HIM) are used for imaging the layers of the sample, and methods such as focused ion beam (FIB) and knife technology (more suitable for biological applications) are used for removing thin layers of the sample to expose the buried layer for imaging 4 , 5 . These 2D images are then stacked to obtain a 3D tomographic image.…”

Section: Introductionmentioning

confidence: 99%

Rapid high-resolution volumetric imaging via laser ablation delayering and confocal imaging

Phoulady

May

Choi

et al. 2022

Sci Rep

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Acquiring detailed 3D images of samples is needed for conducting thorough investigations in a wide range of applications. Doing so using nondestructive methods such as X-ray computed tomography (X-ray CT) has resolution limitations. Destructive methods, which work based on consecutive delayering and imaging of the sample, face a tradeoff between throughput and resolution. Using focused ion beam (FIB) for delayering, although high precision, is low throughput. On the other hand, mechanical methods that can offer fast delayering, are low precision and may put the sample integrity at risk. Herein, we propose to use femtosecond laser ablation as a delayering method in combination with optical and confocal microscopy as the imaging technique for performing rapid 3D imaging. The use of confocal microscopy provides several advantages. First, it eliminates the 3D image distortion resulting from non-flat layers, caused by the difference in laser ablation rate of different materials. It further allows layer height variations to be maintained within a small range. Finally, it enables material characterization based on the processing of material ablation rate at different locations. The proposed method is applied on a printed circuit board (PCB), and the results are validated and compared with the X-ray CT image of the PCB part.

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FIB-SEM Three-Dimensional Tomography for Characterization of Carbon-Based Materials

Cited by 31 publications

References 63 publications

Current Update of Collagen Nanomaterials—Fabrication, Characterisation and Its Applications: A Review

Current Update of Collagen Nanomaterials—Fabrication, Characterisation and Its Applications: A Review

Application of FIB-SEM Techniques for the Advanced Characterization of Earth and Planetary Materials

Rapid high-resolution volumetric imaging via laser ablation delayering and confocal imaging

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