FIB-SEM enhances the potential taxonomic significance of internal pollen wall structure at the generic level

House, Alisoun; Balkwill, K.

doi:10.1016/j.flora.2017.09.011

Cited by 4 publications

(3 citation statements)

References 16 publications

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“…This level of nanoscale resolution in 3D (5 nm×5 nm×5 nm) obtained from FIB-SEM (~20-100 µm maximum slice) is sufficient to visualize all organelles and macromolecular complexes, which offers a powerful capacity for research of subcellular structures at the microscale. In plants, FIB-SEM has been applied to investigate pollen morphology, altered membranes or chloroplasts during viral infection, and deep analysis of cell spatial structure (Risco et al, 2014;Lashbrooke et al, 2015;House and Balkwill, 2017;Jin et al, 2018). In addition, FIB-SEM and 3D reconstruction showed that the mutation of nitrate peptide family (NPF) NPF7.6 in Medicago truncatula leads to wall ingrowths with PMs that had exaggerated jagged margins compared with the wild-type control (Wang et al, 2020c).…”

Section: Volume Electron Microscopymentioning

confidence: 99%

Plant multiscale networks: charting plant connectivity by multi-level analysis and imaging techniques

Zhang

Man

Zhuang

et al. 2021

Sci. China Life Sci.

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In multicellular and even single-celled organisms, individual components are interconnected at multiscale levels to produce enormously complex biological networks that help these systems maintain homeostasis for development and environmental adaptation. Systems biology studies initially adopted network analysis to explore how relationships between individual components give rise to complex biological processes. Network analysis has been applied to dissect the complex connectivity of mammalian brains across different scales in time and space in The Human Brain Project. In plant science, network analysis has similarly been applied to study the connectivity of plant components at the molecular, subcellular, cellular, organic, and organism levels. Analysis of these multiscale networks contributes to our understanding of how genotype determines phenotype. In this review, we summarized the theoretical framework of plant multiscale networks and introduced studies investigating plant networks by various experimental and computational modalities. We next discussed the currently available analytic methodologies and multi-level imaging techniques used to map multiscale networks in plants. Finally, we highlighted some of the technical challenges and key questions remaining to be addressed in this emerging field.

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Section: Volume Electron Microscopymentioning

confidence: 99%

Plant multiscale networks: charting plant connectivity by multi-level analysis and imaging techniques

Zhang

Man

Zhuang

et al. 2021

Sci. China Life Sci.

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“…Although B. albostellata has no recorded practice in traditional medicine, many species of Barleria have been used in traditional medicine and were confirmed to contain various compounds possessing biological effects such as analgesic, anti-inflammatory, antileukemic, antihyperglycemic, antitumor, anti-amoebic, antibiotic, and virucidal activities [21][22][23][24][25][26]. Acanthaceae is regarded as a eurypalynous family [27], with significant diversity in the pollen shape, size, exine structure, apertures, and ornamentation [28][29][30][31][32]. Plants in this family demonstrate a thriving diversity of ecological and morphological characteristics.…”

Section: Introductionmentioning

confidence: 99%

“…These include a large range of pollinator relationships and floral morphologies [33][34][35]. Members of Barleria are easily recognized for their globose, tricolporate pollen with Acanthaceae is regarded as a eurypalynous family [27], with significant diversity in the pollen shape, size, exine structure, apertures, and ornamentation [28][29][30][31][32]. Plants in this family demonstrate a thriving diversity of ecological and morphological characteristics.…”

Section: Introductionmentioning

confidence: 99%

Micromorphology of Barleria albostellata (Grey Barleria) Flower and Pollen Grains

et al. 2023

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Barleria albostellata C.B. Clarke (grey barleria, Acanthaceae) is an indigenous shrub to South Africa and has been relatively understudied. This shrub is a valuable medicinal plant with a wide spectrum of antibacterial and anti-inflammatory activities. Detailed studies on the floral and pollen morphology on B. albostellata are rare. This study was conducted to observe the morphology of the flower and pollen grains using stereomicroscopy and scanning electron microscopy (SEM). Morphological observations showed numerous non-glandular trichomes on the bracteoles and bracts of B. albostellata. Three types of trichomes were identified on these structures: I—unicellular, II—multangulate-dendritic branched non-glandular trichomes, and III—capitate glandular trichomes. A taxonomical description of the floral structures using stereo and SEM micrographs is provided. SEM micrographs revealed the pollen grains as globose tricolporate with a rough honeycomb exine, and small granules inside the lumina. The diameter of the pollen grains was 77.53 ± 5.63 μm, whereas the aperture of these grains was 14.31 ± 0.59 µm. This study provides insight into the floral biology of B. albostellata, and the results presented here will add to the body of knowledge and encourage further research on this species.

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The Triassic pollen genus Camerosporites: New morphological and ultrastructural data, revised taxonomy and paleobiogeographical aspects

Scibiorski

Peyrot²,

Charles³

et al. 2022

Review of Palaeobotany and Palynology

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FIB-SEM enhances the potential taxonomic significance of internal pollen wall structure at the generic level

Cited by 4 publications

References 16 publications

Plant multiscale networks: charting plant connectivity by multi-level analysis and imaging techniques

Plant multiscale networks: charting plant connectivity by multi-level analysis and imaging techniques

Micromorphology of Barleria albostellata (Grey Barleria) Flower and Pollen Grains

The Triassic pollen genus Camerosporites: New morphological and ultrastructural data, revised taxonomy and paleobiogeographical aspects

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