FIA-spectrophotometric determination of nitrazepam by oxidation with a  solid-phase reactor and coupling with 2,2'-dihydroxybiphenyl reagent

Jamal, Marwa K. El; Hadi, Hind

doi:10.4314/bcse.v33i1.18

Cited by 2 publications

(1 citation statement)

References 10 publications

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“…The FIA technique is extensively used for the analysis of a wide spectrum of organic and inorganic compounds [16]. The normal and reverse modes of the various FIA procedures are given a lot of consideration [17,18]. The normal flow injection analysis method (nFIA) entails injecting a small volume of sample into a reagent carrier stream that travels through a thin bore tube to a spectrophotometer, where the derivative is determined.…”

Section: Introductionmentioning

confidence: 99%

Normal and Reverse Flow Injection Analysis Methods for Estimation of Mesalazine in Pharmaceutical Dosage Forms

Hasan,

Hadi

2023

IHJPAS

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Determining the actual amounts of active ingredients in various pharmaceutical commercial forms is still receiving a lot of attention. Two flow injection analysis (FIA) methods were suggested for determination of mesalazine (MES) in pharmaceutical forms. Normal and reverse FIA systems (nFIA and rFIA) combined with UV-Vis spectrophotometric technique were used for the analysis. The methods involved using two mods of FIA systems for measuring a colored product result from coupling of MES with 2,2'-dihydroxybiphenyl after oxidized with sodium periodate in alkaline medium. The absorbance of the red colored dye was measured at maximum wavelength of 500 nm. The calibration graphs for MES were linear in the ranges 2.5-200 and 0.5-60 µg/mL with RSD of best than 3% for both methods respectively. Also the limits of detection were 1.2 and 0.2 µg/mL and limits of quantitation were 3.6 and 0.7 µg/mL of MSL for nFIA and rFIA systems respectively. All physical and chemical conditions of flow systems such as flow rate, reaction coil length and reagent concentrations were carefully studied. The proposed methods were applied for determining MES in four commercial tablets without any interferences.

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Section: Introductionmentioning

confidence: 99%

Normal and Reverse Flow Injection Analysis Methods for Estimation of Mesalazine in Pharmaceutical Dosage Forms

Hasan,

Hadi

2023

IHJPAS

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Determination of Meloxicam Using Direct and Indirect Flow Injection Spectrophotometry

Abed

Hadi

2021

CPA

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Background: Meloxicam is a non-steroidal anti-inflammatory drug, known to inhibit cyclooxygenase, a key enzyme of prostaglandin biosynthesis at the site of inflammation. Objectives: Two sensitive and fast spectrophotometric methods (direct and indirect) using flow injection analysis (FIA) have been described for the assay of meloxicam in pure and in pharmaceutical formulations. Methods: The direct method (method A) depended on the coupling of meloxicam with diazotized procaine benzylpenicillin in alkaline medium, while the indirect method (method B) involved a charge transfer reaction between alkaline hydrolytic product of meloxicam as n-donor with metol (p-methylaminophenol sulfate) as a π- acceptor using sodium periodate as an oxidant. Results: The colored products exhibited absorption maxima at 492 and 656 nm for method A and B respectively. The methods obeyed Beer’s low over concentration ranges of 5-80 and 15-225 µg/mL, respectively. The detection limits were 2.73 and 5.26 µg/mL for direct and indirect methods, respectively. The chemical and physical parameters associated with FIA manifolds were optimized to construct the most sensitive and reproducible results. Conclusion: Methods described herein are cheap, environmental friendly and showed no significant difference with those of official method indicated no significant difference in accuracy and precision.

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FIA-spectrophotometric determination of nitrazepam by oxidation with a solid-phase reactor and coupling with 2,2'-dihydroxybiphenyl reagent

Cited by 2 publications

References 10 publications

Normal and Reverse Flow Injection Analysis Methods for Estimation of Mesalazine in Pharmaceutical Dosage Forms

Normal and Reverse Flow Injection Analysis Methods for Estimation of Mesalazine in Pharmaceutical Dosage Forms

Determination of Meloxicam Using Direct and Indirect Flow Injection Spectrophotometry

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