FGFR2 modulates the Akt/Nrf2/ARE signaling pathway to improve angiotensin II-induced hypertension-related endothelial dysfunction

Jiao, Kai; Su, Peng; Li, Yongling

doi:10.1080/10641963.2023.2208777

Cited by 7 publications

(3 citation statements)

References 38 publications

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“…Among the parameters that become different with sex, there is cell viability: in FHUVECs, there is a small but not significant decrease in cell viability, whereas in male cells, it is significantly increased. This is in line with previous HUVEC cell lines of unknown sex [51,52], but in the majority of cases, it was shown that Ang II induced viability loss in HUVEC cell lines of unknown sex (an exhaustive list of references about this point is reported in Table S3 [ [53][54][55][56][57][58][59][60][61][62][63][64][65][66]). The discrepancy could be attributed to the use of HUVEC cell lines versus the primary cultures we used, and to different methods of culture and viability determination.…”

Section: Discussionsupporting

confidence: 87%

Sex Influence on Autophagy Markers and miRNAs in Basal and Angiotensin II-Treated Human Umbilical Vein Endothelial Cells

Franconi,

Capobianco,

Diana

et al. 2023

IJMS

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Cardiovascular diseases (CVD) display many sex and gender differences, and endothelial dysfunction, angiotensin II (Ang II), and autophagy represent key factors in the autophagic process Therefore, we studied whether Ang II modulates the mentioned processes in a sex-specific way in HUVECs obtained from healthy male and female newborns. In basal HUVECs, the Parkin gene and protein were higher in FHUVECs than in MHUVECs, while the Beclin-1 protein was more expressed in MHUVECs, and no other significant differences were detected. Ang II significantly increases LAMP-1 and p62 protein expression and decreases the expression of Parkin protein in comparison to basal in MHUVECs. In FHUVECs, Ang II significantly increases the expression of Beclin-1 gene and protein, and Parkin gene. The LC3 II/I ratio and LAMP-1 protein were significantly higher in MHUVECs than in FHUVECs, while Parkin protein was significantly more expressed in Ang II-treated FHUVECs than in male cells. Ang II affects the single miRNA levels: miR-126-3p and miR-133a-3p are downregulated and upregulated in MHUVECs and FHUVECs, respectively. MiR-223 is downregulated in MHUVEC and FHUVECs. Finally, miR-29b-3p and miR-133b are not affected by Ang II. Ang II effects and the relationship between miRNAs and organelles-specific autophagy is sex-dependent in HUVECs. This could lead to a better understanding of the mechanisms underlying sex differences in endothelial dysfunction, providing useful indications for innovative biomarkers and personalized therapeutic approaches.

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Section: Discussionsupporting

confidence: 87%

Sex Influence on Autophagy Markers and miRNAs in Basal and Angiotensin II-Treated Human Umbilical Vein Endothelial Cells

Franconi,

Capobianco,

Diana

et al. 2023

IJMS

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“…Thus, a concentration of 100 ng/mL Bevacizumab, 1.6 μM SHP-60, and their combination, which exhibited mild inhibition, and this information was used for subsequent experimental work. The cell viability analysis was carried out using MTT assay which is routinely used for this analysis for the HUVEC cell line and other cells − …”

Section: Resultsmentioning

confidence: 99%

Investigating the Protein-Based Therapeutic Relationship between Honey Protein SHP-60 and Bevacizumab on Angiogenesis: Exploring the Synergistic Effect through In Vitro and In Silico Analysis

Wahid,

Nazeer,

Qadir

et al. 2024

ACS Omega

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Honey is a natural product produced by honeybees, which has been used not only as food but also as a medicine by humans for thousands of years. In this study, 60 kDa protein was purified from Pakistani Sidr honey named as SHP-60 (Sidr Honey Protein-60), and its antioxidant potential and the effect of Bevacizumab with purified protein on in vitro angiogenesis using human umbilical vein endothelial cells (HUVEC) were investigated. We further validated the molecular protein−protein (SHP-60 with Bevacizumab) interactions through in silico analysis. It showed very promising antioxidant activity by reducing 2,2-diphenyl-1-picrylhydrazyl free radicals with a maximum of 83% inhibition at 50 μM and an IC 50 of 26.45 μM statistically significant (**p < 0.01). Angiogenesis is considered a hallmark of cancer, and without it, the tumor cannot grow or metastasize. Bevacizumab, SHP-60, and both in combination were used to treat HUVEC, and the MTT assay was used to assess cell viability. To demonstrate in vitro angiogenesis, HUVEC was grown on Geltrex, and the formation of endotubes was examined using a tube formation assay. HUVEC viability was dose-dependently decreased by Bevacizumab, SHP-60, and both together. Bevacizumab and SHP-60 both inhibited angiogenesis in vitro, and their combination displayed levels of inhibition even higher than those of Bevacizumab alone. We investigated the protein−protein molecular docking interactions and molecular dynamics simulation analysis of MRJP3 (major royal jelly protein 3) similar to SHP-60 in molecular weight with both the heavy chain (HC) and light chain (LC) of Bevacizumab. We found significant interactions between the LC and MRJP3, indicating that ASN468, GLN470, and ASN473 of MRJP3 interact with SER156, SER159, and GLU161 of LC of Bevacizumab. The integration of experimental data and computational techniques is believed to improve the reliability of the findings and aid in future drug design.

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“…The ROS production of REPNs in cells under ultrasound irradiation was detected by using 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA) as an indicator. 29,30 The MDA MB-231 cells were inoculated in 24-well plates and were cultured for 24 h. After washing with PBS, the medium was replaced with a fresh medium containing RB and REPNs ( C RB = 20 μg mL −1 ) and further incubated for 4 h. Then the cells were washed three times with PBS and stained with DAPI for 10 min in the incubator. After being washed with PBS, the medium containing 10 μM of DCFH-DA was added and then incubated for an extra 30 min.…”

Section: Methodsmentioning

confidence: 99%

Integrin α_vβ₃-targeted self-assembled polypeptide nanomicelles for efficacious sonodynamic therapy against breast cancer

Ren,

Yang,

Kong

et al. 2024

Nanoscale

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FGFR2 modulates the Akt/Nrf2/ARE signaling pathway to improve angiotensin II-induced hypertension-related endothelial dysfunction

Cited by 7 publications

References 38 publications

Sex Influence on Autophagy Markers and miRNAs in Basal and Angiotensin II-Treated Human Umbilical Vein Endothelial Cells

Sex Influence on Autophagy Markers and miRNAs in Basal and Angiotensin II-Treated Human Umbilical Vein Endothelial Cells

Investigating the Protein-Based Therapeutic Relationship between Honey Protein SHP-60 and Bevacizumab on Angiogenesis: Exploring the Synergistic Effect through In Vitro and In Silico Analysis

Integrin α_vβ₃-targeted self-assembled polypeptide nanomicelles for efficacious sonodynamic therapy against breast cancer

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FGFR2 modulates the Akt/Nrf2/ARE signaling pathway to improve angiotensin II-induced hypertension-related endothelial dysfunction

Cited by 7 publications

References 38 publications

Sex Influence on Autophagy Markers and miRNAs in Basal and Angiotensin II-Treated Human Umbilical Vein Endothelial Cells

Sex Influence on Autophagy Markers and miRNAs in Basal and Angiotensin II-Treated Human Umbilical Vein Endothelial Cells

Investigating the Protein-Based Therapeutic Relationship between Honey Protein SHP-60 and Bevacizumab on Angiogenesis: Exploring the Synergistic Effect through In Vitro and In Silico Analysis

Integrin αvβ3-targeted self-assembled polypeptide nanomicelles for efficacious sonodynamic therapy against breast cancer

Contact Info

Product

Resources

About

Integrin α_vβ₃-targeted self-assembled polypeptide nanomicelles for efficacious sonodynamic therapy against breast cancer