FGF7 protein expression in human breast carcinomas

Jacquemier, Jocelyne; Sun, Zhenzhu; Penault‐Llorca, Frédérique; Geneix, Jeannine; Devilard, Elizabeth; Adélaı̈de, José; Birnbaum, Daniel

doi:10.1002/(sici)1096-9896(1998110)186:3<269::aid-path187>3.0.co;2-g

Cited by 16 publications

(12 citation statements)

References 33 publications

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“…Our studies suggest that stromally released FGF7 is biologically active, since CM from stromal cells promoted luminal epithelial and myoepithelial cell proliferation in vitro and this could be abrogated by a neutralising antibody to FGF7. Consistent with this, fibroblast feeder layers have been previously shown to facilitate the culture of human and rat mammary epithelial cells (Guo et al 1996, Mann & Borgen 1998 and other studies have demonstrated the existence of epithelial cell growth factors in the CM from breast fibroblast cultures (Ulich et al 1994, Wilson et al 1994, Bansal et al 1997, Jacquemier et al 1998. Furthering these observations, we show that FGF7 is the mitogenic and morphogenic component in the CM from breast fibroblast cultures.…”

Section: Discussionsupporting

confidence: 90%

“…Previous studies using PCR analysis by others (Jacquemier et al 1998) and our group (Bansal et al 1997) have demonstrated the presence of FGF7 mRNA in breast tissues. We have shown that FGF7 mRNA is present in stromal tissues but not in separated epithelial or myoepithelial cells.…”

Section: Introductionmentioning

confidence: 64%

“…FGF7 was also demonstrated to induce proliferation of breast cancer cells (Bansal et al 1997). Conversely, Jacquemier et al (1998) have demonstrated FGF7 protein associated with the most differentiated and least aggressive breast tumours. Despite these previous studies, the origin, cellular targets and mode of action of FGF7 in the breast remain unclear.…”

Section: Introductionmentioning

confidence: 98%

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Fibroblast growth factor 7, secreted by breast fibroblasts, is an interleukin-1beta-induced paracrine growth factor for human breast cells

Palmieri¹,

Roberts-Clark²,

Assadi-Sabet³

et al. 2003

Journal of Endocrinology

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Keratinocyte growth factor/fibroblast growth factor 7 (KGF/FGF7) is known to be a potent growth factor for mammary cells but its origin, cellular targets and mode of action in the breast are unclear. In this study, we carried out studies to determine the localisation of FGF7 and its receptor, and the related growth factor FGF10. We also determined the factors that regulate FGF7 release from stromal cells and the effects of FGF7 on normal and neoplastic breast cells. Using an FGF7-specific antibody which does not react with the FGF7 heparan sulphate proteoglycan (HSPG)-binding site, we showed epithelial and myoepithelial immunohistochemical staining in normal breast sections, and epithelial staining in breast carcinomas. Stromal staining was also detected in some lobular carcinomas as well as a subset of invasive ductal carcinomas. FGF10 and FGF receptor (FGFR)2 immunostaining showed a similar epithelial expression pattern, whereas no stromal staining was observed. We purified normal breast stromal, epithelial and myoepithelial cells and showed that FGF7 stimulated proliferation of both epithelial cell types, but not stromal fibroblasts. We also examined the effects of FGF7 on Matrigel-embedded organoids, containing both epithelial and myoepithelial cells, and showed FGF7 induced an increase in cellular proliferation. Furthermore, conditioned medium derived from stromal cells was shown to increase the proliferation of normal and neoplastic breast epithelial cells, which could be abolished by a neutralising antibody to FGF7. Finally, we showed that interleukin-1 , but not oestradiol or other oestrogen receptor ligands, caused a dose-related FGF7 release. Further results also indicate that the epithelial localisation of FGF7 and FGF10 in breast tissue sections is likely to be due to their binding to their cognate receptor. In summary, our findings suggest that FGF7 is a paracrine growth factor in the breast. FGF7 is produced by the breast stromal fibroblasts and has profound proliferative and morphogenic roles on both epithelial and myoepithelial cells.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 64%

Section: Introductionmentioning

confidence: 98%

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Fibroblast growth factor 7, secreted by breast fibroblasts, is an interleukin-1beta-induced paracrine growth factor for human breast cells

Palmieri¹,

Roberts-Clark²,

Assadi-Sabet³

et al. 2003

Journal of Endocrinology

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“…FGFR1 and FGFR2 have also been shown to contribute to breast cancer development, whereas FGFR4 has been implicated in therapy response. The references used for this figure are: (1) (Theodorou et al 2007), (2) (Peters et al 1989), (3) (Theodorou et al 2004), (4) (MacArthur et al 1995), (5) (Naidu et al 2001), (6) (Fioravanti et al 1997), (7) (Berns et al 1995), (8) (Jacquemier et al 1998), (9) (Bansal et al 1997), (10) (Zammit et al 2002), (11) (Marsh et al 1999), (12) (Adnane et al 1991), (13) (Bange et al 2002).…”

Section: Igf-1 and Igf1r In Cancermentioning

confidence: 99%

Mammary Gland Growth Factors: Roles in Normal Development and in Cancer

Hynes¹,

Watson²

2010

Cold Spring Harbor Perspectives in Biology

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Normal development of the mammary gland proceeds via interactions between the epithelium and the mesenchyme that start during embryogenesis and continue during pubertal outgrowth and differentiation. The function of specific peptide growth factors that bind members of the receptor tyrosine kinase family and the cytokine receptor family are required at each stage. In many cases the peptides are produced in one compartment and act on receptors in the other compartment. One of the striking differences between normal development and cancer is the loss of this cross-talk. Mammary tumor cells often produce a peptide and express the receptor on the same cell leading to autocrine activation of signaling pathways, a mechanism that is characteristic for cancer cells. We will discuss different peptides in the context of normal development and cancer in this review.

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“…21 FGF-7 (keratinocyte growth factor) acting on epithelial cells 22 is a potent mitogen for mammary glands 23,24 , and induces proliferation and motility in breast cancer cells. 25 This led us to explore the influence of 3-OST3A expression on FGF-7-HS interactions and signaling.…”

Section: --Ost3a Affects Fgf--7--hs Interactions and Regulates Fgf--mentioning

confidence: 99%

The heparan sulfate sulfotransferase 3-OST3A (HS3ST3A) is a novel tumor regulator and a prognostic marker in breast cancer

et al. 2016

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The heparan sulfate sulfotransferase 3-OST3A (HS3ST3A) is a novel tumor regulator and a prognostic marker in breast cancer Mao, X.; Gauche, C.; Coughtrie, M. W. H.; Bui, C.; Gulberti, S.; Merhi-Soussi, F. Citation for published version (APA):Mao, X., Gauche, C., Coughtrie, M. W. H., Bui, C., Gulberti, S., Merhi-Soussi, F., ... Fournel-Gigleux, S. (2016). The heparan sulfate sulfotransferase 3-OST3A (HS3ST3A) is a novel tumor regulator and a prognostic marker in breast cancer. Oncogene, 35, 5043-5055. DOI: 10.1038/onc.2016 General rights Copyright and moral rights for the publications made accessible in Discovery Research Portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.• Users may download and print one copy of any publication from Discovery Research Portal for the purpose of private study or research.• You may not further distribute the material or use it for any profit-making activity or commercial gain.• You may freely distribute the URL identifying the publication in the public portal. Take down policyIf you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. Heparan sulfate (HS) proteoglycan chains are key components of the breast tumor microenvironment that critically influence the behavior of cancer cells. It is established that abnormal synthesis and processing of HS play a prominent role in tumorigenesis albeit mechanisms remain mostly obscure. HS function is mainly controlled by sulfotransferases, and here we report a novel cellular and pathophysiological significance for the 3--O--sulfotransferase 3--OST3A (HS3ST3A), catalyzing the final maturation step of HS, in breast cancer. We show that 3--OST3A is epigenetically repressed in all breast cancer cell lines of a panel representative of distinct molecular subgroups, except in HER2--positive (HER2+) SKBR3 cells. Epigenetic mechanisms involved both DNA methylation and histone modifications, producing different repressive chromatin environments depending on the cell molecular signature. Gain and loss of function experiments by cDNA and siRNA transfection revealed profound effects of 3--OST3A expression on cell behavior including apoptosis, proliferation, response to trastuzumab in vitro and tumor growth in xenografted mice. 3--OST3A exerted dual activities acting as tumor--suppressor in lumA--MCF--7 and triple negative--MDA--MB--231 cells, or as an oncogenic factor in HER2+--SKBR3 cells. Mechanistically, fluorescence--resonance energy transfer (FRET)--fluorescence--lifetime imaging microscopy (FLIM) experiments indicated that the effects of 3--OST3A in MCF--7 cells were mediated by altered interactions between HS and fibroblast growth factor--7 (FGF--7). Further, this interplay between HS and FGF--7 modulated downstream ERK, AKT and p38 cascades, suggesting that altering 3--O--sulfation affects FGFR...

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FGF7 protein expression in human breast carcinomas

Cited by 16 publications

References 33 publications

Fibroblast growth factor 7, secreted by breast fibroblasts, is an interleukin-1beta-induced paracrine growth factor for human breast cells

Fibroblast growth factor 7, secreted by breast fibroblasts, is an interleukin-1beta-induced paracrine growth factor for human breast cells

Mammary Gland Growth Factors: Roles in Normal Development and in Cancer

The heparan sulfate sulfotransferase 3-OST3A (HS3ST3A) is a novel tumor regulator and a prognostic marker in breast cancer

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