FGF2, FGF3 and FGF4 expression pattern during molars odontogenesis in Didelphis albiventris

Santos, Íria Gabriela Dias dos; Jorge, Érika Cristina; Copola, Aline Gonçalves Lio; Bertassoli, Bruno Machado; Góes, Alfredo M.; Silva, Gerluza Aparecida Borges

doi:10.1016/j.acthis.2016.12.001

Cited by 7 publications

(4 citation statements)

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“…Our group has been trying to establish D. albiventris as a model for understanding odontogenesis stages during development. This opossum exhibits complete heterodont dentition that is closer to that of humans than is that of rodents, the typical model for these studies [8,[51][52][53][54][55]. We can characterize the morphological stages of early tooth development (dental lamina, bud, cap, and bell stages) in this species [8,[51][52][53][54][55].…”

Section: Introductionmentioning

confidence: 99%

Didelphis albiventris: an overview of an unprecedented transcriptome sequencing of the white-eared opossum

Santos

Mendes

Silva

et al. 2019

Preprint

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BackgroundThe white-eared opossum (Didelphis albiventris) is widely distributed throughout Brazil and South America. It has been used as an animal model for studying different scientific questions ranging from the restoration of degraded green areas to medical aspects of Chagas disease, leishmaniasis and resistance against snake venom. As a marsupial, D. albiventris can also contribute to the understanding of the molecular mechanisms that govern the different stages of organogenesis. Opossum joeys are born after only 13 days, and the final stages of organogenesis occur when the neonates are inside the pouch, depending on lactation. As neither the genome of this opossum species nor its transcriptome has been completely sequenced, the use of D. albiventris as an animal model is limited. In this work, we sequenced the D. albiventris transcriptome by RNA-seq to obtain the first catalogue of differentially expressed (DE) genes and gene ontology (GO) annotations during the neonatal stages of marsupial development.ResultsThe D. albiventris transcriptome was obtained from whole neonates harvested at birth (P0), at five days of age (P5) and at ten days of age (P10). The de novo assembly of these transcripts generated 85,338 transcripts. Approximately 30% of these transcripts could be mapped against the amino acid sequences of M. domestica, the evolutionarily closest relative of D. albiventris to be sequenced thus far. Among the expressed transcripts, 2,077 were found to be DE between P0 and P5, 13,780 between P0 and P10, and 1,453 between P5 and P10. The enriched GO terms were mainly related to the immune system, blood tissue development and differentiation, vision, hearing, digestion, the CNS and limb development.ConclusionsThe elucidation of opossum transcriptomes provides an out-group for better understanding the distinct characteristics associated with the evolution of mammalian species. This study provides the first transcriptome sequences and catalogue of genes for a marsupial species at different neonatal stages, allowing the study of the mechanisms involved in organogenesis.

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Section: Introductionmentioning

confidence: 99%

Didelphis albiventris: an overview of an unprecedented transcriptome sequencing of the white-eared opossum

Santos

Mendes

Silva

et al. 2019

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Our group has been trying to establish D. albiventris as a model for understanding odontogenesis stages during development. This opossum exhibits complete heterodont dentition that is closer to that of humans than is that of rodents, the typical model for these studies [8, 51–55]. We can characterize the morphological stages of early tooth development (dental lamina, bud, cap, and bell stages) in this species [8, 51–55].…”

Section: Introductionmentioning

confidence: 99%

“…This opossum exhibits complete heterodont dentition that is closer to that of humans than is that of rodents, the typical model for these studies [8, 51–55]. We can characterize the morphological stages of early tooth development (dental lamina, bud, cap, and bell stages) in this species [8, 51–55]. However, the use of sequences from M. domestica (the closest evolutionary relative) to develop molecular approaches to study D. albiventris has not been successful.…”

Section: Introductionmentioning

confidence: 99%

Didelphis albiventris: an overview of unprecedented transcriptome sequencing of the white-eared opossum

et al. 2019

Self Cite

View full text Add to dashboard Cite

BackgroundThe white-eared opossum (Didelphis albiventris) is widely distributed throughout Brazil and South America. It has been used as an animal model for studying different scientific questions ranging from the restoration of degraded green areas to medical aspects of Chagas disease, leishmaniasis and resistance against snake venom. As a marsupial, D. albiventris can also contribute to the understanding of the molecular mechanisms that govern the different stages of organogenesis. Opossum joeys are born after only 13 days, and the final stages of organogenesis occur when the neonates are inside the pouch, depending on lactation. As neither the genome of this opossum species nor its transcriptome has been completely sequenced, the use of D. albiventris as an animal model is limited. In this work, we sequenced the D. albiventris transcriptome by RNA-seq to obtain the first catalogue of differentially expressed (DE) genes and gene ontology (GO) annotations during the neonatal stages of marsupial development.ResultsThe D. albiventris transcriptome was obtained from whole neonates harvested at birth (P0), at 5 days of age (P5) and at 10 days of age (P10). The de novo assembly of these transcripts generated 85,338 transcripts. Approximately 30% of these transcripts could be mapped against the amino acid sequences of M. domestica, the evolutionarily closest relative of D. albiventris to be sequenced thus far. Among the expressed transcripts, 2077 were found to be DE between P0 and P5, 13,780 between P0 and P10, and 1453 between P5 and P10. The enriched GO terms were mainly related to the immune system, blood tissue development and differentiation, vision, hearing, digestion, the CNS and limb development.ConclusionsThe elucidation of opossum transcriptomes provides an out-group for better understanding the distinct characteristics associated with the evolution of mammalian species. This study provides the first transcriptome sequences and catalogue of genes for a marsupial species at different neonatal stages, allowing the study of the mechanisms involved in organogenesis.

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“…D. albiventris and other didelphids are also known to be resistant to the venom of snakes, such as Bothrops spp., Crotalus durissus and Lachesis muta [32][33][34]; and they are also resistant to intoxication by millipedes [35], which is toxic for many vertebrates. Additionally, our group has been trying to establish D. albiventris as a model to understand the odontogenesis stages of development, as this opossum has a complete heterodont dentition, the closest to humans in relation to rodents, the typical model for these studies [4,[36][37][38][39][40]. We could characterize the morphological stages of early tooth development (dental lamina, bud, cap, and bell stages) in this species [4,[36][37][38][39][40]; but the use of the M. domestica sequences (the closest evolutive relative) to develop molecular approaches to study D. albiventris was not successful.…”

mentioning

confidence: 99%

Didelphis albiventris: an overview of an unprecedented transcriptome sequencing of the white-eared opossum

Santos

Mendes

Silva

et al. 2019

Preprint

Self Cite

View full text Add to dashboard Cite

Background The white-eared opossum (Didelphis albiventris) is widely distributed throughout Brazil and South America. They have been used as animal models to study different aspects in science, from the restoration of degraded green areas to medical aspects of the Chagas disease, leishmaniasis and resistance against snake venom. As a marsupial, D. albiventris can also aid the comprehension of the molecular mechanisms that govern the different stages of organogenesis, as their joeys are born after only 13 days, depending on placentation, and the final stages of organogenesis occurs when the neonates are inside the pouch, depending on lactation. As the genome of this opossum species, and/or its transcriptome, had not been completely sequenced yet, the use of D. albiventris as an animal model is limited. In this work, we have sequenced the D. albiventris transcriptome, by RNA-seq, to obtain the first catalogue of differentially expressed genes (DE) and gene ontology (GO) annotation during the neonatal stages of the marsupial development. Results The D. albiventris transcriptome was obtained from whole neonates harvested at birth (P0), at five days old (P5) and ten days old (P10). The de novo assembly of these transcripts allowed us to obtain 85,338 transcripts. Only ~30% of these transcripts could be mapped against M. domestica genome, the closest phylogenetic relative with available nucleotide sequences. Among the expressed transcripts, 2,077 were found to be DE between P0 and P5, 13,780 between P0 and P10, and 1,453 between P5 and P10. Enriched GO terms were mainly related to immune system, blood tissue development and differentiation, vision, hearing, digestion, CNS and limb development. Conclusions The unveiling of opossum transcriptomes provides an out-group to better understand the distinct characteristics associated with the evolution of mammalian species. Nevertheless, this is the first transcriptome sequencing and available catalogue of genes of a marsupial species at different neonatal stages, allowing the study of mechanisms involved in the organogenesis.

show abstract

FGF2, FGF3 and FGF4 expression pattern during molars odontogenesis in Didelphis albiventris

Cited by 7 publications

References 70 publications

Didelphis albiventris: an overview of an unprecedented transcriptome sequencing of the white-eared opossum

Didelphis albiventris: an overview of an unprecedented transcriptome sequencing of the white-eared opossum

Didelphis albiventris: an overview of unprecedented transcriptome sequencing of the white-eared opossum

Didelphis albiventris: an overview of an unprecedented transcriptome sequencing of the white-eared opossum

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