FGF2 and FGFR2 in patients with idiopathic pulmonary fibrosis and lung cancer

Abstract

The aim of this study was to investigate the expression of FGF2 and FGFR2 in patients with idiopathic pulmonary fibrosis (IPF) and lung cancer (LC) as well as their clinical significance. Reverse transcription-quantitative PCR (RT-qPCR) and western blotting were used to detect FGF2 and FGFR2 expression in LC and adjacent normal tissues of LC patients and lavage fluid of idiopathic pulmonary fibers patients and normal controls (confirmed by bronchoalveolar lavage examination). The expression levels of FGF2 mRNA and protein in the non-small cell LC tissues were significantly higher than those in the adjacent normal tissues (P<0.001). The expression level of FGF2 protein in lavage fluid of patients with IPF was higher than that of the control group (P<0.001). The expression level of FGFR2 mRNA in the non-small cell LC tissues was significantly higher than that in the adjacent normal tissues (P<0.001). The expression level of FGFR2 protein in the non-small cell LC tissues was higher than that in the adjacent normal lung tissues (P<0.001). The expression levels of FGF2 mRNA and FGFR2 mRNA in cancer tissues were not significantly correlated with age, sex and history of smoking (P>0.05), but were significantly correlated with lymph node metastasis, tumor differentiation and TNM staging. FGF2 and FGFR2 proteins were highly expressed in cancer tissues of LC patients and lavage fluid of patients with IPF. The expression of FGF2 mRNA and FGFR2 mRNA was correlated with lymph node metastasis and TNM stage. The high expression levels of FGF2 mRNA and FGFR2 mRNA were associated with tumor metastasis and poor prognosis of LC patients.

Dysregulated Lung Commensal Bacteria Drive Interleukin-17B Production to Promote Pulmonary Fibrosis through Their Outer Membrane Vesicles

“…Human lavage fluid was collected from 10 IPF patients and 9 normal controls in Shanghai Pulmonary Hospital as previously reported (Li et al, 2018). This study was approved by the Medical Ethics Committee of Shanghai Pulmonary Hospital (Shanghai, China), and all patients were informed and signed informed consent.…”

Novel immune subtypes of lung adenocarcinoma identified through bioinformatic analysis

“…These data were used to study the relationship between 15 genes and TME among patients with high versus low immune scores or stromal score groups using the ESTIMATE algorithm. Previous studies have reported that 9 out of the 15 immune genes associated with prognosis (S100A16, CRABP1, RBP2, FGF2, FPR2, BDNF, ANGPTL4, SEMA4B and VIPR1 [33][34][35][36][37][38][39][40][41]) are involved in the pathogenesis of lung cancer. The remaining six genes (IGKV4-1, IGKV6D-41, TNFRSF11A, INHA, IL11 and SCH3) have not been reported in the literature and may be used as potential biomarkers for lung cancer research.…”

Short-term exposure to intermittent hypoxia leads to changes in gene expression seen in chronic pulmonary disease

“…As another example, IH downregulated fibroblast growth factor receptor (FGFR-2) in alveolar type 2 cells. FGFR2 promotes alveolar regeneration in response to lung injury (56), and is upregulated in patients with idiopathic pulmonary fibrosis (57). Previous studies suggest that OSA can induce injury to the lung (58,59), and OSA is prevalent in patients with idiopathic pulmonary fibrosis (36).…”