FGF signaling directs a center-to-pole expansion of tubulogenesis in mouse testis differentiation

Hiramatsu, Ryuji; Harikae, Kyoko; Tsunekawa, Naoki; Kurohmaru, Masamichi; Matsuo, Isao; Kanai‐Azuma, Masami

doi:10.1242/dev.040519

Cited by 81 publications

(72 citation statements)

References 49 publications

(64 reference statements)

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“…The sections (4 mm thickness) were subjected to conventional histological (hematoxylin and eosin; H&E) and immunohistochemical staining as described previously (Hiramatsu et al 2010, Harikae et al 2013b.…”

Section: Histology and Immunohistochemistrymentioning

confidence: 99%

A novel Amh-Treck transgenic mouse line allows toxin-dependent loss of supporting cells in gonads

Shinomura¹,

Kishi²,

Tomita³

et al. 2014

Reproduction

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Cell ablation technology is useful for studying specific cell lineages in a developing organ in vivo. Herein, we established a novel anti-Mü llerian hormone (AMH)-toxin receptor-mediated cell knockout (Treck) mouse line, in which the diphtheria toxin (DT) receptor was specifically activated in Sertoli and granulosa cells in postnatal testes and ovaries respectively. In the postnatal testes of Amh-Treck transgenic (Tg) male mice, DT injection induced a specific loss of the Sertoli cells in a dose-dependent manner, as well as the specific degeneration of granulosa cells in the primary and secondary follicles caused by DT injection in Tg females. In the testes with depletion of Sertoli cell, germ cells appeared to survive for only several days after DT treatment and rapidly underwent cell degeneration, which led to the accumulation of a large amount of cell debris within the seminiferous tubules by day 10 after DT treatment. Transplantation of exogenous healthy Sertoli cells following DT treatment rescued the germ cell loss in the transplantation sites of the seminiferous epithelia, leading to a partial recovery of the spermatogenesis. These results provide not only in vivo evidence of the crucial role of Sertoli cells in the maintenance of germ cells, but also show that the Amh-Treck Tg line is a useful in vivo model of the function of the supporting cell lineage in developing mammalian gonads.

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Section: Histology and Immunohistochemistrymentioning

confidence: 99%

A novel Amh-Treck transgenic mouse line allows toxin-dependent loss of supporting cells in gonads

Shinomura¹,

Kishi²,

Tomita³

et al. 2014

Reproduction

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“…Nevertheless, regardless of speculation on this somatic role, our results suggest that goat SRY may have functions other than up-regulating SOX9, at least in non-Sertoli somatic cells. As for the second question, as it has been shown in mice that the maintenance of Sox9 expression requires two independent positive feed forward loops, one engaging Fgf9 and Fgfr2 (Kim et al, 2006;Bagheri-Fam et al, 2008;Hiramatsu et al, 2010), and the other Pgds and PGD2 (Wilhelm et al, 2005;Moniot et al, 2009), so we tried to evaluate these pathways in the goat. Of interest, FGFR2 was found to be expressed specifically in differentiating Sertoli cells at the time of SOX9 up-regulation.…”

Section: Primary Expression Time Of Switchingmentioning

confidence: 99%

“…Consequently, the first steps of testis differentiation appear to be straightforward in the mouse: pre-Sertoli cells specifically express SRY that directly activates transcription of the Sox9 gene (Sekido and Lovell-Badge, 2008). Once SOX9 is up-regulated in the Sertoli cells, its expression is maintained by at least two positive feedback loops, one that involves Fgf9 and its receptor Fgfr2 (Kim et al, 2006;Bagheri-Fam et al, 2008;Hiramatsu et al, 2010), and the other which acts through the Pgds gene and its by-product PGD2 (Wilhelm et al, 2005;Moniot et al, 2009). Concomitantly to the maintenance of Sox9 expression, Sry expression declines and then disappears completely after seminiferous cord formation at around 12.5 dpc (Wilhelm et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

A study of goat SRY protein expression suggests putative new roles for this gene in the developing testis of a species with long‐lasting SRY expression

Montazer-Torbati

Kocer

Auguste

et al. 2010

Developmental Dynamics

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The testis-determining gene SRY is not well-conserved among mammals, and particularly between mouse and other mammals. To evaluate SRY function in a nonrodent species, we produced an antibody against goat SRY and used it to investigate the expression pattern of SRY throughout goat testicular development. By contrast with the mouse, SRY is primarily expressed in most cells of XY genital-ridges and not solely in pre-Sertoli cells. Between cord formation and prepuberty, SRY remains expressed in both Sertoli and germinal cells. During adulthood, SRY expression declines and then disappears from meiotic germ cells, only remaining present at low levels in some spermatogonia. Unlike the germinal lineage, SRY continues to be highly expressed in adult Sertoli cells with a typical nuclear staining. Our data indicate that in goat, the role of SRY may not be limited to testis determination and could have other functions in testicular maintenance and hence male fertility. Developmental Dynamics 239:3324-3335,

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“…FGF9 is known as glia-activating factor, 15 and is involved in numerous processes, such as differentiation of soft tissues 16 and testis, 17 sex determination, 18 angiogenesis and osteogenesis in long bone repair. 19 Furthermore, FGF9 has an important role in the progression of several malignant diseases.…”

Section: Discussionmentioning

confidence: 99%

Relationship between the localization of fibroblast growth factor 9 in prostate cancer cells and postoperative recurrence

Teishima

Shoji

Hayashi

et al. 2011

Prostate Cancer Prostatic Dis

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BACKGROUND: Fibroblast growth factor 9 (FGF9) enhances cell proliferation and invasiveness in several malignant diseases. The aim of the present study is to investigate the role of FGF9 in postoperative recurrence after radical prostatectomy. METHODS:Cell viability and invasion of LNCaP cells were assessed using MTT assay and Matrigel invasion assay, respectively, in the presence or absence of treatment with recombinant FGF9. Tissues obtained during a radical prostatectomy in 133 male patients were immunohistochemically stained using anti-FGF9 antibody. RESULTS:Cell viability and invasion of LNCaP was significantly enhanced by treatment with recombinant FGF9. Immunohistochemical staining detected FGF9-positive cells in 20 samples. The prevalence of FGF9-positive cells in cases with a Gleason score of 8 or higher was 34.2%, which was significantly higher than that in those with Gleason scores of 7 or lower (7.3%, P ¼ 0.0003), respectively. The 3-year biochemical relapse-free survival rate was 17.5% in cases with FGF9-positive cells, which was significantly lower than that in cases in which FGF9-positive cells were not detectable (75.5%, Po0.0001). CONCLUSIONS:These results indicate that FGF9 can stimulate proliferation and invasion in prostate cancer cells, thus FGF9 could be a candidate of a predictive factor for recurrence after radical prostatectomy.

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FGF signaling directs a center-to-pole expansion of tubulogenesis in mouse testis differentiation

Cited by 81 publications

References 49 publications

A novel Amh-Treck transgenic mouse line allows toxin-dependent loss of supporting cells in gonads

A novel Amh-Treck transgenic mouse line allows toxin-dependent loss of supporting cells in gonads

A study of goat SRY protein expression suggests putative new roles for this gene in the developing testis of a species with long‐lasting SRY expression

Relationship between the localization of fibroblast growth factor 9 in prostate cancer cells and postoperative recurrence

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