FGF-2 Regulates Enamel and Dentine Formation in Mouse Tooth Germ

Tsuboi, Takahiro; Mizutani, Shigeki; Nakano, Masaya; Hirukawa, Koji; Togari, Akifumi

doi:10.1007/s00223-002-4070-2

Cited by 38 publications

(33 citation statements)

References 33 publications

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“…In the case of pulpodentin complex, inductive growth may come from the regeneration of degraded dentin matrix [79], from revitalization process or synthesized in vitro and delivered into tissues [80]. Among them it is worth mention [81] recombinant human bone morphogenetic proteins-2 and -4 or rh BMP2 and rh BMP4 [82,83]; stromal cell derived factor (SDF)-1, basic fibroblast growth factor (BFGF) [84,85,86], platelet derived growth factor (PDGF), stem cells factor (SCF), granulocyte colony-stimulating factor (G-CSF), and VEGF proangiogenic factor [87,88]. …”

Section: Main Usable Growth Factors and Signaling Moleculesmentioning

confidence: 99%

Nanomaterials for Tissue Engineering In Dentistry

et al. 2016

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The tissue engineering (TE) of dental oral tissue is facing significant changes in clinical treatments in dentistry. TE is based on a stem cell, signaling molecule, and scaffold triad that must be known and calibrated with attention to specific sectors in dentistry. This review article shows a summary of micro- and nanomorphological characteristics of dental tissues, of stem cells available in the oral region, of signaling molecules usable in TE, and of scaffolds available to guide partial or total reconstruction of hard, soft, periodontal, and bone tissues. Some scaffoldless techniques used in TE are also presented. Then actual and future roles of nanotechnologies about TE in dentistry are presented.

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Section: Main Usable Growth Factors and Signaling Moleculesmentioning

confidence: 99%

Nanomaterials for Tissue Engineering In Dentistry

et al. 2016

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“…FGF2 regulates tooth morphogenesis by controlling cell proliferation and differentiation. 74 FGF2 is a potent angiogenic factor stimulating new blood vessel formation in the dental pulp 75 along with PDGF 19,20 and VEGF. 66 Given its role in cell proliferation and angiogenesis, FGF2 acts as an early stimulating factor in granulation tissue formation during wound healing.…”

Section: Fibroblast Growth Factor (Fgf)mentioning

confidence: 99%

Effects of Growth Factors on Dental Stem/Progenitor Cells

Kim

Zhou

Solomon

et al. 2012

Dental Clinics of North America

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Synopsis The primary goal of regenerative endodontics is to restore the vitality and functions of the dentin-pulp complex, as opposed to filing of the root canal with bioinert materials. Structural restoration is also important but is likely secondary to vitality and functions. Myriads growth factors regulate multiple cellular functions including migration, proliferation, differentiation and apoptosis of several cell types that are intimately involved in dentin-pulp regeneration: odontoblasts, interstitial fibroblasts, vascular-endothelial cells and sprouting nerve fibers. Recent work showing that growth factor delivery, without cell transplantation, can yield pulp-dentin like tissues in vivo provides one of the tangible pathways for regenerative endodontics. This review synthesizes our knowledge on a multitude of growth factors that are known or anticipated to be efficacious in dental pulp-dentin regeneration.

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“…Several studies showed that FGF2 inhibited dentinogenesis and the expression of dentin sialophosphoprotein ( Dspp ) [Tsuboi et al, 2003; Shimabukuro et al, 2009; Xiao et al, 2009; Kim et al, 2010; Kim et al, 2014]. On the other hand, other studies showed that FGF2 stimulated the expression of Dspp in vitro and the formation of osteodentin in vivo [Kikuchi et al, 2007; Ishimatsu et al, 2009; Kim et al, 2010; Kim et al, 2014].…”

Section: Introductionmentioning

confidence: 99%

Stage-Specific Effects of Fibroblast Growth Factor 2 on the Differentiation of Dental Pulp Cells

Sagomonyants¹,

Mina²

2014

Cells Tissues Organs

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Dentinogenesis is a complex and multistep process, which is regulated by various growth factors, including members of the fibroblast growth factor (FGF) family. Both positive and negative effects of FGFs on dentinogenesis have been reported, but the underlying mechanisms of these conflicting results are still unclear. To gain a better insight into the role of FGF2 in dentinogenesis, we used dental pulp cells from various transgenic mice, in which fluorescent protein expression identifies cells at different stages of odontoblast differentiation. Our results showed that the continuous exposure of pulp cells to FGF2 inhibited mineralization and revealed both the stimulatory and inhibitory effects of FGF2 on the expression of markers of dentinogenesis and various transgenes. During the proliferation phase of in vitro growth, FGF2 increased the expression of markers of dentinogenesis and the percentages of dentin matrix protein 1/green fluorescent protein (DMP1-GFP)-positive functional odontoblasts and dentin sialophosphoprotein (DSPP)-Cerulean-positive odontoblasts. Additional exposure to FGF2 during the differentiation/mineralization phase of in vitro growth decreased the extent of mineralization and the expression of markers of dentinogenesis and of the DMP1-GFP and DSPP-Cerulean transgenes. Recovery experiments showed that the inhibitory effects of FGF2 on dentinogenesis were related to the blocking of the differentiation of cells into mature odontoblasts. These observations together showed the stage-specific effects of FGF2 on dentinogenesis by dental pulp cells, and they provide critical information for the development of improved treatments for vital pulp therapy and dentin regeneration.

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FGF-2 Regulates Enamel and Dentine Formation in Mouse Tooth Germ

Cited by 38 publications

References 33 publications

Nanomaterials for Tissue Engineering In Dentistry

Nanomaterials for Tissue Engineering In Dentistry

Effects of Growth Factors on Dental Stem/Progenitor Cells

Stage-Specific Effects of Fibroblast Growth Factor 2 on the Differentiation of Dental Pulp Cells

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