Fg<scp>K</scp>in1 kinase localizes to the septal pore and plays a role in hyphal growth, ascospore germination, pathogenesis, and localization of <scp>T</scp>ub1 beta‐tubulins in <i><scp>F</scp>usarium graminearum</i>

Luo, Yongping; Zhang, Hongchang; Qi, Linlu; Zhang, Shijie; Zhou, Xiaoying; Zhang, Yimei; Xu, Jin‐Rong

doi:10.1111/nph.12953

Cited by 42 publications

(60 citation statements)

References 61 publications

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“…This defect is similar to that of the Fgkin1 mutant 19 . Kin1 is a MARK kinase that is involved in microtubule based transportation via phosphorylation of microtubule-associated proteins 25 .…”

Section: Discussionsupporting

confidence: 72%

“…7A). Similar to amd1 , germination of ascospores from one end also was observed inside Fgkin1 perithecia 19 (Fig. 7A).…”

Section: Resultssupporting

confidence: 67%

“…graminearum , FgKin1, a microtubule affinity-regulating protein kinase (MARK), is also required for ascospore discharge and prevention of ascospore germination inside perithecia 19 . The Fgkin1 and amd1 mutants has similar defects in ascospore discharge and disintegration of the ascus wall (Fig.…”

Section: Resultsmentioning

confidence: 99%

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RNA editing of the AMD1 gene is important for ascus maturation and ascospore discharge in Fusarium graminearum

Cao

Hao

et al. 2017

Sci Rep

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Ascospores are the primary inoculum in the wheat scab fungus Fusarium graminearum that was recently shown to have sexual stage-specific A-to-I RNA editing. One of the genes with premature-stop-codons requiring A-to-I editing to encode full-length functional proteins is AMD1 that encodes a protein with a major facilitator superfamily (MFS) domain. Here, we characterized the functions of AMD1 and its UAG to UGG editing event. The amd1 deletion mutant was normal in growth and conidiation but defective in ascospore discharge due to the premature breakdown of its ascus wall in older perithecia, which is consistent with the specific expression of AMD1 at later stages of sexual development. Expression of the wild-type or edited allele of AMD1 but not un-editable allele rescued the defects of amd1 in ascospore discharge. Furthermore, Amd1-GFP localized to the ascus membrane and Amd1 orthologs are only present in ascocarp-forming fungi that physically discharge ascospores. Interestingly, deletion of AMD1 results in the up-regulation of a number of genes related to transporter activity and membrane functions. Overall, these results indicated that Amd1 may play a critical role in maintaining ascus wall integrity during ascus maturation, and A-to-I editing of its transcripts is important for ascospore discharge in F. graminearum.

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Section: Discussionsupporting

confidence: 72%

“…7A). Similar to amd1 , germination of ascospores from one end also was observed inside Fgkin1 perithecia 19 (Fig. 7A).…”

Section: Resultssupporting

confidence: 67%

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RNA editing of the AMD1 gene is important for ascus maturation and ascospore discharge in Fusarium graminearum

Cao

Hao

et al. 2017

Sci Rep

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“…To assay for possible defects in septation and nuclear distribution, we transformed the H1-GFP fusion construct [35] into the Δ gil1 mutant T10 and the wild type strain PH-1. Vegetative hyphae of the transformants of PH-1 or T10 expressing H1-GFP were stained with Calcofluor White (CFW) as described [13].…”

Section: Resultsmentioning

confidence: 99%

A Gin4-Like Protein Kinase GIL1 Involvement in Hyphal Growth, Asexual Development, and Pathogenesis in Fusarium graminearum

Zhang

et al. 2017

IJMS

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Fusarium graminearum is the main causal agent of Fusarium head blight (FHB) on wheat and barley. In a previous study, a GIN4-like protein kinase gene, GIL1, was found to be important for plant infection and sexual reproduction. In this study we further characterized the functions of GIL1 kinase in different developmental processes. The Δgil1 mutants were reduced in growth, conidiation, and virulence, and formed whitish and compact colonies. Although phialide formation was rarely observed in the mutants, deletion of GIL1 resulted in increased hyphal branching and increased tolerance to cell wall and cell membrane stresses. The Δgil1 mutants produced straight, elongated conidia lacking of distinct foot cells and being delayed in germination. Compared with the wild type, some compartments in the vegetative hyphae of Δgil1 mutants had longer septal distances and increased number of nuclei, suggesting GIL1 is related to cytokinesis and septation. Localization of the GIL1-GFP fusion proteins to the septum and hyphal branching and fusion sites further supported its roles in septation and branching. Overall, our results indicate that GIL1 plays a role in vegetative growth and plant infection in F. graminearum, and is involved in septation and hyphal branching.

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“…The production of DON is regulated not only by TRI , but also by protein kinases [23]. There are about 120 predicted protein kinase genes in F. graminearum .…”

Section: Introductionmentioning

confidence: 99%

Screening of Deoxynivalenol Producing Strains and Elucidation of Possible Toxigenic Molecular Mechanism

Zheng

Zhang

Zhao

et al. 2017

Toxins

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In this study, seven strains of Fusarium graminearum were isolated from wheat, of which six were identified to produce deoxynivalenol and the production of deoxynivalenol was assessed. F. graminearum strain Fg1 was noted to produce 1.0 μg/g deoxynivalenol during the incubation period in the Czapek yeast broth, while none was detected in F. graminearum strain Fg2. Hence, the differences in proteomes and transcriptomes of Fg1 and Fg2 were compared to analyze the mechanism underlying deoxynivalenol production. Among the 66 significantly differentially expressed proteins in Fg1, 39 and 27 were more or less abundant expressed. Functional analysis suggested that the enzymes involved in the methylerythritol 4-phosphate and mevalonate pathways, which provide a substrate for biosynthesis of farnesyl pyrophosphate, a precursor of DON, were activated in Fg1. The transcriptomics data demonstrated that the expression level of a majority of genes, including trichothecene biosynthetic genes, protein kinases, and transcription factors, involved in trichothecene biosynthesis was higher in Fg1 than in Fg2. The results also revealed differential expression profiles of deoxynivalenol biosynthesis genes in strains Fg1 and Fg2, which emphasized their deoxynivalenol producing ability and the underlying mechanism.

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FgKin1 kinase localizes to the septal pore and plays a role in hyphal growth, ascospore germination, pathogenesis, and localization of Tub1 beta‐tubulins in Fusarium graminearum

Cited by 42 publications

References 61 publications

RNA editing of the AMD1 gene is important for ascus maturation and ascospore discharge in Fusarium graminearum

RNA editing of the AMD1 gene is important for ascus maturation and ascospore discharge in Fusarium graminearum

A Gin4-Like Protein Kinase GIL1 Involvement in Hyphal Growth, Asexual Development, and Pathogenesis in Fusarium graminearum

Screening of Deoxynivalenol Producing Strains and Elucidation of Possible Toxigenic Molecular Mechanism

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