Abstract:Foram utilizados quatro cavalos castrados, por meio de delineamento em blocos casualizados. Objetivou-se viabilizar a obtenção in vitro das fibras indigestíveis, FDNi e FDAi, mediante a utilização, como inóculos, de líquido ruminal (LR) e fezes equina (FE), para estimar o coeficiente de digestibilidade nutrientes (CDN) de cavalos. Os tratamentos foram constituídos pelo método direto com a coleta total de fezes (CT) e indireto pelo uso das FDNi e FDAi obtidas por meio dos inóculos, LR e FE. Desta forma, no prim… Show more
“…In an extensive review, Foster et al [36] observed that even if all the variables of the animals used as a source of ruminal inoculum were controlled, the cell wall components of the feed are the main components that could affect the digestibility of dry matter or organic matter in in vitro studies. When equine feces were used as an alternative inoculum source in in vitro trials, significant differences between ruminal inoculum sources were observed for IVDMD and IVNDFD [41]. The objective of the principal component analysis is to reduce the number of variables found in the relative contribution method (Singh method) to a smaller number of orthogonal variables that represent the greatest degree of variation in the study.…”
The objective of the current study was to compare the rumen inoculum of slaughtered cattle with the ruminal inoculum of cannulated cattle; additionally, determine its reliability in the in vitro analysis of ruminant feeds throughout a multivariate approach. Five male bovines (weight 320 ± 9.4 kg; mean ± standard deviation) provided with ruminal cannula and between five and seven bovines slaughtered in slaughterhouse were used. The evaluations were carried out following a completely randomized design. The data obtained were subjected to different multivariate analyzes to determine the reliability of the ruminal inoculum of animals slaughtered in commercial slaughterhouses compared to that obtained from cannulated animals. The relative contribution indicated that the in vitro dry matter digestibility (IVDMD, 50.75%) and in vitro neutral detergent fiber digestibility (IVNDFD, 29.83%) analysis influence 80.13% of the results to determine the reliability of the ruminal inoculum from slaughtered cattle. Furthermore, it was determined that the first two principal components (IVNDFD and acetic acid production) are the ones that influence the results by 89.87%. The grouping of diets using the Tocher optimization method and the dendrogram shows the formation of six groups and two groups, respectively. The grouping shows that the ruminal inoculum source was not the limiting parameter in the evaluation. Rumen inoculum from cattle slaughtered in a commercial slaughterhouse (with unknown diet) has potential as an alternative for the in vitro analysis of cattle feed, provided that the lignin concentration in the diet is less than 35.5 g/kg DM.
“…In an extensive review, Foster et al [36] observed that even if all the variables of the animals used as a source of ruminal inoculum were controlled, the cell wall components of the feed are the main components that could affect the digestibility of dry matter or organic matter in in vitro studies. When equine feces were used as an alternative inoculum source in in vitro trials, significant differences between ruminal inoculum sources were observed for IVDMD and IVNDFD [41]. The objective of the principal component analysis is to reduce the number of variables found in the relative contribution method (Singh method) to a smaller number of orthogonal variables that represent the greatest degree of variation in the study.…”
The objective of the current study was to compare the rumen inoculum of slaughtered cattle with the ruminal inoculum of cannulated cattle; additionally, determine its reliability in the in vitro analysis of ruminant feeds throughout a multivariate approach. Five male bovines (weight 320 ± 9.4 kg; mean ± standard deviation) provided with ruminal cannula and between five and seven bovines slaughtered in slaughterhouse were used. The evaluations were carried out following a completely randomized design. The data obtained were subjected to different multivariate analyzes to determine the reliability of the ruminal inoculum of animals slaughtered in commercial slaughterhouses compared to that obtained from cannulated animals. The relative contribution indicated that the in vitro dry matter digestibility (IVDMD, 50.75%) and in vitro neutral detergent fiber digestibility (IVNDFD, 29.83%) analysis influence 80.13% of the results to determine the reliability of the ruminal inoculum from slaughtered cattle. Furthermore, it was determined that the first two principal components (IVNDFD and acetic acid production) are the ones that influence the results by 89.87%. The grouping of diets using the Tocher optimization method and the dendrogram shows the formation of six groups and two groups, respectively. The grouping shows that the ruminal inoculum source was not the limiting parameter in the evaluation. Rumen inoculum from cattle slaughtered in a commercial slaughterhouse (with unknown diet) has potential as an alternative for the in vitro analysis of cattle feed, provided that the lignin concentration in the diet is less than 35.5 g/kg DM.
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