Few Layer Graphene Does Not Affect Cellular Homeostasis of Mouse Macrophages

Malanagahalli, Sowmya; Murera, Diane; Martín, Cristina; Lin, Hazel; Wadier, Nadége; Dumortier, Hélène; Vázquez, Éster; Bianco, Alberto

doi:10.3390/nano10020228

Cited by 18 publications

(17 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results also suggest that there is either no or insignificant oxidative stress after treatment with GP. This corresponds with the findings of several studies pointing to a possible hemocompatibility of pristine GP, where, despite its intracellular persistence, there was no release of IL-6, TNFα and IL-1β in either short-term or long-term exposures [31,53]. On the contrary, oxidized forms of graphene, like GO, have the capacity to induce a pro-inflammatory response via oxidative stress, a leading mechanism of cytotoxicity [35,54].…”

Section: Inflammatory Potentialsupporting

confidence: 90%

In Vitro Assessment of the Genotoxic Potential of Pristine Graphene Platelets

et al. 2021

View full text Add to dashboard Cite

(1) Background: Graphene is a two-dimensional atomic structure with a wide range of uses, including for biomedical applications. However, knowledge of its hazards is still limited. This work brings new cytotoxic, cytostatic, genotoxic and immunotoxic data concerning the in vitro exposure of human cell line to two types of graphene platelets (GP). It also contributes to the formation of general conclusions about the health risks of GP exposure. (2) Methods: In vitro exposure of a THP-1 cell line to three concentrations of two GP over 40 h. The cytotoxic potential was assessed by the measurement of LDH and glutathione (ROS) and by a trypan blue exclusion assay (TBEA); the cytostatic and genotoxic potential were assessed by the cytokinesis-block micronucleus (CBMN) test; and the immunotoxic potential was assessed by the measurement of IL-6, IL-10 and TNF-α. (3) Results: We found a significant dose-dependent increase in DNA damage (CBMN). The lowest observed genotoxic effect levels (LOGEL) were 5 µg/mL (GP1) and 30 µg/mL (GP2). We found no significant leaking of LDH from cells, increase in dead cells (TBEA), induction of ROS, increased levels of cytostasis, or changes in IL-6, IL-10 and TNF-α levels. (4) Conclusions: The genotoxicity increased during the short-term in vitro exposure of THP-1 to two GP. No increase in cytotoxicity, immunotoxicity, or cytostasis was observed.

show abstract

Section: Inflammatory Potentialsupporting

confidence: 90%

In Vitro Assessment of the Genotoxic Potential of Pristine Graphene Platelets

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Similar results shed light on the impact of FLG on primary macrophages and show that FLG does not elicit immunological responses leading to cell death [31]. On the other hand, the use of immortalized cell lines is a commonly used model for toxicological evaluation of nanomaterials.…”

mentioning

confidence: 54%

Toxicological evaluation of highly water dispersible few-layer graphene in vivo

Ruiz

Lucherelli

Murera

et al. 2020

Carbon

Self Cite

View full text Add to dashboard Cite

In the last decade, graphene-based materials have received increasing attention for both academic research and industrial uses. However, products containing graphene must meet the same standards for quality, safety and efficacy as products not containing nanomaterials. Our aim is to shed light on the toxicological characterization of few-layer graphene (FLG) dispersions. In the present study, graphene was easily dispersed in water using biocompatible riboflavin-5′-phosphate sodium salt (Rib). A highly concentrated FLG dispersion (G-Rib) was stable for months. G-Rib suspension was characterized by HR-TEM, Raman spectroscopy and ζ-potential. Our results showed that even at high concentration of G-Rib, cell survival was always above 85%. Then, we investigated the tissue distribution and toxic effects of G-Rib in mice up to 30 days after intravenous injections. Histological analysis of the tissues revealed hepatic accumulation and excretion through the kidneys. The biochemical and hematological parameters remained within the reference range showing no hematotoxicity. Finally, no signs of inflammation were detected in the cells isolated from the lymph nodes and spleen. The results of the study show that highly water dispersed FLG is a material with a very low toxic profile, which is one of the first requirements for the future development of biomedical applications.

show abstract

“…Malanagahalli et al prepared few-layer graphene (FLG), a derivative of graphene, and studied its immunomodulatory effects on mouse bone marrow-derived macrophages. This study reported that FLG exerted its immunomodulatory effects after entering into the macrophages by passive diffusion [ 179 ]. Graphene is also reported to suppress dendritic cells which are responsible to stimulate adaptive immune cells such as T and B cells in response to a foreign antigen [ 180 ].…”

Section: Application Of Cnms In Cardiovascular Tissue Engineeringmentioning

confidence: 99%

Carbon nanomaterials for cardiovascular theranostics: Promises and challenges

Alagarsamy

Mathan

Yan

et al. 2021

Bioactive Materials

View full text Add to dashboard Cite

Cardiovascular diseases (CVDs) are the leading cause of death worldwide. Heart attack and stroke cause irreversible tissue damage. The currently available treatment options are limited to “damage-control” rather than tissue repair. The recent advances in nanomaterials have offered novel approaches to restore tissue function after injury. In particular, carbon nanomaterials (CNMs) have shown significant promise to bridge the gap in clinical translation of biomaterial based therapies. This family of carbon allotropes (including graphenes, carbon nanotubes and fullerenes) have unique physiochemical properties, including exceptional mechanical strength, electrical conductivity, chemical behaviour, thermal stability and optical properties. These intrinsic properties make CNMs ideal materials for use in cardiovascular theranostics. This review is focused on recent efforts in the diagnosis and treatment of heart diseases using graphenes and carbon nanotubes. The first section introduces currently available derivatives of graphenes and carbon nanotubes and discusses some of the key characteristics of these materials. The second section covers their application in drug delivery, biosensors, tissue engineering and immunomodulation with a focus on cardiovascular applications. The final section discusses current shortcomings and limitations of CNMs in cardiovascular applications and reviews ongoing efforts to address these concerns and to bring CNMs from bench to bedside.

show abstract

Few Layer Graphene Does Not Affect Cellular Homeostasis of Mouse Macrophages

Cited by 18 publications

References 35 publications

In Vitro Assessment of the Genotoxic Potential of Pristine Graphene Platelets

In Vitro Assessment of the Genotoxic Potential of Pristine Graphene Platelets

Toxicological evaluation of highly water dispersible few-layer graphene in vivo

Carbon nanomaterials for cardiovascular theranostics: Promises and challenges

Contact Info

Product

Resources

About