We present few-femtosecond shadowgraphic snapshots taken during the non-linear evolution of the plasma wave in a laser wakefield accelerator with transverse synchronized few-cycle probe pulses. These snapshots can be directly associated with the electron density distribution within the plasma wave and give quantitative information about its size and shape. Our results show that self-injection of electrons into the first plasma wave period is induced by a lengthening of the first plasma period. Three dimensional particle in cell simulations support our observations.Laser-wakefield accelerators (LWFA) operating in the 'bubble'-regime [1] can generate quasimonoenergetic multigigaelectronvolt electron beams [2,3] with femtosecond duration [4,5] and micrometer dimensions [6,7]. These beams are produced by accelerating electrons in laser-driven plasma waves over centimeter distances. They have the potential to be compact alternatives to conventional accelerators [8]. In a LWFA, the short driving laser pulse displaces plasma electrons from the stationary background ions. The generated space charge fields cause the electrons to oscillate and form a plasma wave in the laser's wake. This wave follows the laser at almost c, the speed of light; for low amplitude it has a wavelength ofwhere n e is the electron density of the plasma. At high amplitude, electrons from the background can be injected into the wake and accelerated, producing monoenergetic electron pulses [9][10][11]. Significant progress has been made regarding achievable peak energy [3], beam stability [12] and the generation of bright X-ray pulses [13][14][15]. Until now, most of our knowledge about the dynamics of the self-injection process has been derived from detailed particle-in-cell (PIC) simulations. These simulations show that self-focusing [16] and pulse compression [17] play a vital role in increasing the laser pulse intensity prior to injection. Furthermore, simulations indicate that self-injection of electrons is associated with a dynamic lengthening of the first plasma wave's period (the 'bubble'). This lengthening can be driven by changes of the electric field structure inside the plasma wave caused by the injected electrons [18]. In contrast, the lengthening may also be due to an intensity amplification of the laser pulse caused by the non-linear evolution of the plasma wave [19,20] or due to a local increase in intensity caused by two colliding pulses [21]. In these latter scenarios, injection is a consequence of the lengthening of the bubble. However, experimental insight into these processes is extremely challenging due to the small spatial and temporal scales of a LWFA.The plasma wave, a variation in the electron density, has an associated refractive index profile which can be detected using longitudinal [22][23][24] or transverse probes [5]. Longitudinal probes cannot measure the rapid and dynamic evolution of the plasma wave that occurs in nonlinear wakefield accelerators and suffer from the strong refraction caused by the steep refractive ...