Feulgen-hydrolysis profiles in cells exfoliated from the cervix uteri: a potential aid in the diagnosis of malignancy.

Millett, J; Husain, O. A. N.; Bitensky, Lucille; Chayen, J.

doi:10.1136/jcp.35.3.345

Cited by 26 publications

(13 citation statements)

References 16 publications

(14 reference statements)

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“…These would not be contradictory to our results. Our findings are also consistent with the similar, though diminished, lability to Feulgen hydrolysis of cytologically normal cells in smears from cervical cancer patients compared to their counterparts in smears from normal patients [58].…”

Section: Dna Histograms In the Presence Of Heparinsupporting

confidence: 94%

Mitotic rate, DNA distribution, and chromatinin situ sensitivity to heparin in breast cancer

Weiß¹,

Brasching²,

Bock

et al. 1990

Breast Cancer Res Tr

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The purpose of this study was to characterize breast carcinomas by cell kinetic parameters. Mitotic rate (MR) and flow cytometrically (FCM) measured cell cycle distribution as well as chromatin testing in situ employing heparin for determination of activated chromatin, provided the following results: MR counted in 73 unselected carcinomas showed an increase up to a tumor size of 4.2 cm (p less than 0.05); beyond this diameter, the MR was found to decrease. In T1-T2 carcinomas, cell cycle stage analysis yielded higher percentages of cells in S and G2M phase for ductal (13% and 12%, N = 22) than for lobular (8% and 7%, N = 8) node-negative carcinomas (p less than 0.002). In ductal carcinomas, lymph node involvement was reflected by higher % G2M values (15%, N = 26) compared with negative cases (12%, N = 22) (p less than 0.05). Ductal node-positive T3-T4 carcinomas (N = 10) revealed a higher % S value (16%) than their T1-T2 counterparts. A correlation between MR and % G2M was established only up to a tumor size of 4.2 cm (r = 0.39, p less than 0.05). A highly sensitive ('H') and a poorly sensitive ('P') subgroup of carcinomas with respect to heparin-induced changes in fluorescence intensity of the G1/0 peak of the DNA aneuploid cell line were identified, as previously shown. These subgroups were here updated with a larger number of carcinomas and were limited to T1-T2 cancers (N = 57). Group 'H' included more younger patients (p less than 0.005), less cases with nodal involvement in ductal carcinomas (p less than 0.05), and lower % G2M values in lobular node-negative cases (p less than 0.05), than group 'P'. DNA diploid cells always existing in DNA aneuploid carcinomas are more sensitive than their aneuploid counterparts (p less than 0.01); however, they strengthen the stratification to 'H' and 'P'. We suggest 'H' carcinomas to be less aggressive than 'P' carcinomas. Small breast carcinomas are recommended to cell kinetic investigations for individualizing adjuvant therapy.

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Section: Dna Histograms In the Presence Of Heparinsupporting

confidence: 94%

Mitotic rate, DNA distribution, and chromatinin situ sensitivity to heparin in breast cancer

Weiß¹,

Brasching²,

Bock

et al. 1990

Breast Cancer Res Tr

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“…Sandritter showed that CIS of the uterine cervix is an aneuploid lesion and postulated an increasing DNA content as normal squamous epithelium progresses to invasive carcinoma. 18 -23 In 98% of squamous cell carcinomas of the uterine cervix, DNA aneuploidy as the cytometric equivalent of cytogenetic aneuploidy can be detected, 16,24,25 but so far it has not been found in normal or in reactive squamous cells of the cervix. 20,26,27 Precancerous lesions of the uterine cervix show cytogenetic aneuploidy 28 as a marker of transition into aneuploid carcinomas.…”

Section: Discussionmentioning

confidence: 99%

DNA cytometry confirms the utility of the Bethesda system for the classification of Papanicolaou smears

Bollmann¹,

Bollmann²,

Henson³

et al. 2001

Cancer

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“…The increased nuclear expression of GST was consistent, in that the intenincreasedĩn al bu on:aeo;CN oee, enoeria A. incraseds inells but onecasualosbe ofCNtowiever,y endocervical cells visually in a cervical smear preparation, but an automated process would require a second marker. For example, nuclear GST positive cells could be evaluated for DNA content (Millet et al, 1982), or endocervical cells discounted by staining for an antibody specific for simple epithelium. The expression of GST by immature squamous metaplasia is particularly disappointing as this lesion may be mistaken for CIN on both cytology and histology.…”

Section: Discussionmentioning

confidence: 99%

Glutathione S-transferase (placental) as a marker of transformation in the human cervix uteri: an immunohistochemical study

Randall

Angus²,

Akiba³

et al. 1990

Br J Cancer

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Summary Using an indirect immunohistochemical technique on paraffin sections, employing a polyclonal antibody to the acidic (placental) form of glutathione-S-transferase (GST), we have evaluated cytoplasmic and nuclear staining in a series of 67 cervical biopsies including normal non neoplastic tissue, immature squamous metaplasia, all grades of cervical intraepithelial neoplasia (CIN) and invasive carcinomas of keratinising and non-keratinising types. No differences in cytoplasmic staining between the varied lesions studied were seen. However, there were marked differences in nuclear staining. While normal non-neoplastic stratified squamous epithelium showed weak staining of the lower one-third of the epithelium only, in immature squamous metaplasia and in all grades of CIN there was intense nuclear staining in all layers of the epithelium. Invasive carcinomas showed generally less intense nuclear staining than CIN lesions. Endocervical cell nuclei also showed intense nuclear staining. These findings indicate that GST is of limited use as a marker of transformation in the human cervix uteri.

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Feulgen-hydrolysis profiles in cells exfoliated from the cervix uteri: a potential aid in the diagnosis of malignancy.

Cited by 26 publications

References 16 publications

Mitotic rate, DNA distribution, and chromatinin situ sensitivity to heparin in breast cancer

Mitotic rate, DNA distribution, and chromatinin situ sensitivity to heparin in breast cancer

DNA cytometry confirms the utility of the Bethesda system for the classification of Papanicolaou smears

Glutathione S-transferase (placental) as a marker of transformation in the human cervix uteri: an immunohistochemical study

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